Expression of telomerase &amp; its significance in the diagnosis of pancreatic cancer

Zisuh, Anutebeh Verdo; Han, Tao; Zhan, Shen-Dao

doi:10.4103/0971-5916.93420

Cited by 14 publications

(3 citation statements)

References 35 publications

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“…Human telomerase reverse transriptase (hTERT), a part of telomerase and crucial subunit for enzyme activity, pretend to be a marker of PDAC development. Its expression, even better than telomerase activity, predict low outcome in individuals with PC 26.…”

Section: Nuclear Dna Changesmentioning

confidence: 99%

An Overview of Genetic Changes and Risk of Pancreatic Ductal Adenocarcinoma

Sarnecka

Zagozda

Durlik³

2016

J. Cancer

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The pancreatic carcinoma is a leading cause of death in cancer carriers worldwide. The early diagnostic is difficult due to late stage during diagnosis, lack of characteristic symptoms and also multifactor basis. In cancer development take part both, environmental and genetic factors, alone or in conjunction with each other. The nonspecific biomarkers of cancers are a reason for the search for more accurate factors which allow for fast and personalized diagnostics. Some of cancers have identified molecular (metabolic, biochemical or genetic) markers but in most cases the only clue is patient`s interview and abnormal levels of organ functions markers.Possible genetic basis of cancer suggests to widen studies on connection between environmental factors with both, nuclear and mitochondrial, genes changes.

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Section: Nuclear Dna Changesmentioning

confidence: 99%

An Overview of Genetic Changes and Risk of Pancreatic Ductal Adenocarcinoma

Sarnecka

Zagozda

Durlik³

2016

J. Cancer

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“…Expression of 95 per cent telomerase activity has been detected in pancreatic adenocarcinoma. Thereby, the detection of telomerase activity has been proposed to be a useful tool in the diagnosis of pancreatic cancer 70 . To include telomere as CAD biomarkers, TL measurement variation needs to be reduced that presently restricts our means to perceive consistent result in TL assessment.…”

Section: Discussionmentioning

confidence: 99%

Telomere length as a potential biomarker of coronary artery disease

Bhattacharyya¹,

Mihara

Bhattacharjee³

et al. 2017

Indian J Med Res

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Coronary artery disease (CAD) is a multifactorial disease whose prevalence remains unabated especially in developing countries. Both lifestyle factors and genetic predisposition contribute to this disorder. Though notable achievements have been made in the medical, interventional and surgical management of CAD, the need for its prevention is more important. Among other modalities, this calls for defining evidence-based new biomarkers, which on their own or in combination with other known biomarkers may predict the risk of CAD to enable institution of appropriate preventive strategies. In the present communication, we have discussed the usefulness of shortening of telomeres as a potential biomarker of CAD. Clinical research evidence in favour of telomere shortening in CAD is well documented in different ethnic populations of the world. Establishing a well-standardized and accurate method of evaluating telomere length is essential before its routine use in preventive cardiology.

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“…Telomeres are maintained by telomerase, a multi-subunit enzyme with an RNA component, which acts as a template for making telomeric repeats and a protein reverse transcriptase component, hTERT, which catalyzes the synthesis of hexameric repeats TTAGGG8. Most of the cancer cells reactivate telomerase activity9, and it may be the target for treating cancers10. Although several studies have documented synthesis of chitosan-coated PLGA-loaded hTERT siRNA nanoparticle, but its effect on cancer cells remains unexplored.…”

mentioning

confidence: 99%

Knockdown of human telomerase reverse transcriptase induces apoptosis in cervical cancer cell line

et al. 2019

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Background & objectives: Human telomerase reverse transcriptase (hTERT) is the catalytic subunit of telomerase enzyme that maintains telomere ends by the addition of telomeric repeats to the ends of chromosomal DNA, and that may generate immortal cancer cells. Hence, the activity of telomerase is raised in cancer cells including cervical cancer. The present study aimed to validate the unique siRNA loaded chitosan coated poly-lactic-co-glycolic acid (PLGA) nanoparticle targeting hTERT mRNA to knock down the expression of hTERT in HeLa cells. Methods: The siRNA loaded chitosan coated polylactic-co-glycolic acid (PLGA) nanoparticles were synthesized by double emulsion solvent diffusion method. The characterization of nano-formulation was done to determine efficient siRNA delivery. MTT [3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide] assay, reverse transcriptase-polymerase chain reaction (RT-PCR) and Western blot were performed to evaluate silencing efficiency of nano-formulation. Results: Size, zeta potential and encapsulation efficiency of nanoparticles were 249.2 nm, 12.4 mV and 80.5 per cent, respectively. Sustained release of siRNA from prepared nanoparticle was studied for 72 h by ultraviolet method. Staining assays were performed to confirm senescence and apoptosis. Silencing of hTERT mRNA and protein expression were analyzed in HeLa cells by RT-PCR and Western blot. Interpretation & conclusions: The findings showed that biodegradable chitosan coated PLGA nanoparticles possessed an ability for efficient and successful siRNA delivery. The siRNA-loaded PLGA nanoparticles induced apoptosis in HeLa cells. Further studies need to be done with animal model.

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Expression of telomerase & its significance in the diagnosis of pancreatic cancer

Cited by 14 publications

References 35 publications

An Overview of Genetic Changes and Risk of Pancreatic Ductal Adenocarcinoma

An Overview of Genetic Changes and Risk of Pancreatic Ductal Adenocarcinoma

Telomere length as a potential biomarker of coronary artery disease

Knockdown of human telomerase reverse transcriptase induces apoptosis in cervical cancer cell line

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