Expression of TECK/CCL25 and MEC/CCL28 chemokines and their respective receptors CCR9 and CCR10 in porcine mucosal tissues

Meurens, François; Berri, Mustapha; Whale, Julia; Dybvig, Tova; Strom, Stacy; Thompson, David R.; Brownlie, Robert; Townsend, H. G. G.; Salmon, Henri; Gerdts, Volker

doi:10.1016/j.vetimm.2006.05.014

Cited by 42 publications

(48 citation statements)

References 36 publications

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“…Consequently very low CCR10 mRNA expression in GF and LF groups suggest a reduction in the migration of plasmablasts and lymphocytes to the gut even if there is evidence of a low expression of CCL28 mRNA in the tissue. As previously observed CCR10 mRNA expression does not strictly follow CCL28 mRNA expression [12]. CCL28 has two important roles in mucosal immunity; chemokine and antimicrobial peptide [47].…”

Section: Discussionmentioning

confidence: 53%

“…Contrary to what we observed with CCL25, there were no significant differences between SI locations in EC and CV groups. The lower expression of CCL25 mRNA in the last segment could be explained by the presence of Peyer's patches (PP) in this location since we have usually observed [12], lower expression of CCL25 in PP than in the rest of the SI. In the pig there are two types of PP: several separate patches in the jejunum and proximal ileum, and a long continuous patch in the distal ileum [1], [2].…”

Section: Discussionmentioning

confidence: 92%

“…However, very little is known about the influence of commensal bacteria on CCL25 and CCL28 expression, two majors intestinal chemokines implicated in lymphocytes trafficking to the gut. In this study, taking advantage of a recently developed germ-free pig model and a previous study in pig [12], [33], we assessed mRNA expression of CCL25, CCL28, CCR9, CCR10, Foxp3, TGF-β and IL-10 at 5 regions corresponding to 5%, 25%, 50%, 75%, and 95% of the SI length following colonization with different bacterial species.…”

Section: Discussionmentioning

confidence: 99%

“…In addition, chemokine receptor 9 (CCR9), the main receptor for CCL25, is specifically expressed on a subset of gut-homing T cells expressing integrin α4β7, as well as on IgA-secreting cells from gastrointestinal organs [8], [9], [15], [16], [19], [20]. CCL28, also called mucosae-associated epithelial chemokine (MEC), is expressed in most human, mouse, pig and sheep mucosal tissues including the salivary gland, mammary gland, small and large intestines and trachea, where it appears to be predominantly produced by epithelial cells [12], [13], [21], [22]. Cells expressing CCR10 are mainly IgA and IgM plasmablasts and some T lymphocytes [22]–[26].…”

Section: Introductionmentioning

confidence: 99%

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Commensal Bacteria and Expression of Two Major Intestinal Chemokines, TECK/CCL25 and MEC/CCL28, and Their Receptors

et al. 2007

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BackgroundCCL25/TECK and CCL28/MEC are CC chemokines primarily expressed in thymic dendritic cells and mucosal epithelial cells. Their receptors, CCR9 and CCR10, are mainly expressed on T and B lymphocytes. In human, mouse, pig and sheep CCL25 and CCL28 play an important role in the segregation and the compartmentalization of the mucosal immune system. As evidenced by early comparisons of germ-free and conventional animals, the intestinal bacterial microflora has a marked effect on host intestinal immune functions. However, little is known about the impact of bacterial colonization on constitutive and induced chemokine expressions as well as on the generation of anti-inflammatory mechanisms.Methodology/Principal FindingsTherefore, we decided to focus by qPCR on the mRNA expression of two main gut chemokines, CCL25 and CCL28, their receptors CCR9 and CCR10, the Tregs marker Foxp3 and anti-inflammatory cytokines TGF-β and IL-10 following colonization with different bacterial species within the small intestine. To accomplish this we used an original germ-free neonatal pig model and monoassociated pigs with a representative Gram-negative (Escherichia coli) or Gram-positive (Lactobacillus fermentum) commensal bacteria commonly isolated from the neonatal pig intestine. Our results show a consistent and marked effect of microbial colonization on the mRNA expression of intestinal chemokines, chemokine receptors, Foxp3 and TGF-β. Moreover, as evidenced by in vitro experiments using two different cell lines, the pattern of regulation of CCL25 and CCL28 expression in the gut appears complex and suggests an additional role for in vivo factors.Conclusions/SignificanceTaken together, the results highlight the key role of bacterial microflora in the development of a functional intestinal immune system in an elegant and relevant model for human immune system development.

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Section: Discussionmentioning

confidence: 53%

Section: Discussionmentioning

confidence: 92%

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Commensal Bacteria and Expression of Two Major Intestinal Chemokines, TECK/CCL25 and MEC/CCL28, and Their Receptors

et al. 2007

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“…CCL28/CCR10: CCL28 also known as MEC (mucosaeassociated epithelial chemokine), is expressed by epithelial cells of most mucosal tissues including the small and large intestines, the salivary gland, mammary gland, and trachea [28]. CCR10 is mainly expressed by IgA and IgM plasmablasts and some T lymphocytes [29].…”

Section: Introductionmentioning

confidence: 99%

Chemokines and their Receptors in Gut Homeostasis and Disease

Kolios¹,

Lira²

2011

CIR

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The intestinal mucosa, the innermost layer of the bowel wall, is involved in various immunological and inflammatory processes orchestrated by a large number of mucosal immune cells and migrated leukocytes. Migration of leukocytes into the intestinal mucosa appears to be regulated by chemokines, a large group of chemotactic cytokines produced by multiple cell types including epithelial cells, endothelial cells and leukocytes. They mediate their activities by binding to an array of shared and specific chemokine receptors on leukocytes. Here we review the evidence supporting a role for chemokines and their receptors in gut homeostasis and disease, and discuss their potential use in therapy of inflammatory bowel diseases.

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Interferon as a Mucosal Adjuvant for an Influenza Vaccine in Pigs

et al. 2019

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Interferon, a natural protein that is produced by a variety of cells during viral infection, activates the transcription of multiple functional genes in cells, regulates synergy among various signaling pathways, and mediates many biological functions such as antiviral activity, immune regulation, and cell growth. However, clinical research on interferon in livestock is lacking. In this study, recombinant porcine interferon (PoIFNa) was used as an adjuvant, in combination with inactivated influenza virus, to vaccinate 6-week-old pigs via nasal infusion. The transcription of target genes was then monitored and the functions of PoIFNa were determined with respect to the activation of mucosal immunity. We found that a combination of low-dose PoIFNa and inactivated influenza virus could significantly up-regulate the expression of immunoregulatory cytokines such as IL-2, IL-18, IFN-c, IL-6, and IL-10 by real-time PCR, suggesting the induction of a strong mucosal innate immune response after administration. In addition, low-dose PoIFNa can significant enhancing the transcription of genes encoding homing factors including CCR9 and CCR10 (P \ 0.001), thereby resulting in the induction of higher levels of HA-specific antibodies (P \ 0.05), which can be determined by ELISA and IFA. Post-immunization challenges with H1N1 virus demonstrated that PoIFNa, combined with inactivated influenza virus, could alleviate clinical signs in pigs during the early stages of viral infection. These studies reveal low-dose PoIFNa as a potential mucosal adjuvant for influenza virus in pigs. Keywords Porcine interferon a (PoIFNa) Á H1N1 influenza virus Á Intranasal administration Á Cytokines Electronic supplementary material The online version of this article (

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Expression of TECK/CCL25 and MEC/CCL28 chemokines and their respective receptors CCR9 and CCR10 in porcine mucosal tissues

Cited by 42 publications

References 36 publications

Commensal Bacteria and Expression of Two Major Intestinal Chemokines, TECK/CCL25 and MEC/CCL28, and Their Receptors

Commensal Bacteria and Expression of Two Major Intestinal Chemokines, TECK/CCL25 and MEC/CCL28, and Their Receptors

Chemokines and their Receptors in Gut Homeostasis and Disease

Interferon as a Mucosal Adjuvant for an Influenza Vaccine in Pigs

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