Expression of Synthetic pac Gene Encoding Penicillin G Acylase (PGA) Enzyme in E. coli BL21(DE3) and HB101
Achmad Makin Amin,
Sismindari Sismindari,
Purwanto Purwanto
Abstract:High bacterial infection cases in Indonesia cause a high need for antibiotic drugs. Unfortunately, most of the raw materials used for antibiotic production in Indonesia are still imported. For this reason, the government is eager to find better ways to produce penicillin and its derivatives, which are widely used in society. The production of penicillin-derivative requires penicillin G acylase (PGA) as a catalyst. In previous studies, the expression of the syn-pac gene in E. coli BL21(DE3) to produce a recombi… Show more
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