Expression of Syk and MAP4 proteins in ovarian cancer

Abstract

PurposeWe have previously reported on the prognostic importance of the calpain family of proteins in ovarian cancer, especially calpain-2. Spleen tyrosine kinase (Syk) phosphorylates a variety of cytoskeletal proteins with studies suggesting potential interactions between Syk and conventional calpains. Microtubule-associated protein 4 (MAP4) has been reported to be regulated by Syk.MethodsThe current study assessed Syk and MAP4 protein expression, by immunohistochemistry on a tissue microarray comprised of cores from primary ovarian carcinomas (n = 575), to evaluate associations with patient clinical outcomes and other clinicopathological factors and sought to determine whether there were any correlations between the expression of Syk, MAP4 and the calpain system.ResultsMAP4 expression was significantly associated with ovarian cancer histological subtype (P < 0.001), stage (P = 0.001), grade (P < 0.001) and residual tumour (P = 0.005). Despite this finding, we found no significant association existing between MAP4 expression and overall survival. Syk expression was also found significantly associated with histological subtype (P < 0.001). Syk seems to play a contradictory role with respect to tumour progression: low cytoplasmic Syk expression was significantly associated with low stage (P = 0.013), and low nuclear Syk expression with chemo-resistance in patients treated with taxane-containing therapy (P = 0.006). Interestingly, despite the lack of association in the whole cohort, high nuclear Syk expression was significantly associated with better overall survival in certain subgroups (P = 0.001).ConclusionsThe current study indicates a lack of correlation between calpain-2 expression and Syk and MAP4. Syk, MAP4 and calpain-1 appeared to significantly correlate with each other in the whole cohort, with calpain-1 being more highly associated with MAP4 and Syk in mucinous carcinomas. Overall, the current results suggest that Syk, MAP4, and calpain-1 expression are correlated with each other and these proteins may be involved in early stages of tumour spread.Electronic supplementary materialThe online version of this article (10.1007/s00432-019-02856-9) contains supplementary material, which is available to authorized users.

Dopamine and cAMP‐regulated phosphoprotein 32kDa (DARPP‐32), protein phosphatase‐1 and cyclin‐dependent kinase 5 expression in ovarian cancer

“…Immunohistochemical staining was conducted using a Novolink Polymer Detection kit (Leica) on a tissue microarray comprised of single 0.6mm cores from 575 ovarian tumours taken from a representative area as assessed by a specialist ovarian cancer histopathologist; the use of which has been described previously. [27][28][29] Staining was conducted according to manufacturers' instructions and has been described previously. 30 Briefly, tissue was deparaffinized in xylene, rehydrated in ethanol then water and heated in a microwave for 10 minutes at 750 W followed by 10 minutes at 450 W in 0.01 mol L −1 sodium citrate buffer (pH6.0).…”

Evaluation of SYK Gene as a Prognostic Biomarker and Suggested Aromatic Potential Phytochemicals to Halt the Colorectal Cancer

“…Thus, there is a positive correlation between SYK expression and increase risk of tumor metastasis formation. Several reports also demonstrate that the role of the SYK gene in diverse cancer types as a potential prognostic marker like in human breast carcinomas [55], cervical carcinogenesis [56], human lung cancer [57], ovarian cancer [58], and neck [59], glioblastoma [60], etc. Here, the divergent role of SYK in cancer may be due to phosphorylation or methylation at the promoter region.…”

Comprehensively investigating the expression levels and the prognostic role of transforming growth factor beta-induced (TGFBI) in glioblastoma multiforme

“…The expression of LCK was a risk factor of tumor recurrence for CC ( 42 ). Syk was detectable in ovarian cancer ( 43 ). Further, the ATM kinase, ATR kinase, and CDK2 even could severe as the anticancer targets ( 44 , 45 ).…”