Expression of Surface Protein LapB by a Wide Spectrum of Listeria monocytogenes Serotypes as Demonstrated with Anti-LapB Monoclonal Antibodies

Boivin, Teela; Elmgren, C.; Brooks, Brian W.; Huang, Hongsheng; Pagotto, Franco; Lin, Min

doi:10.1128/aem.01908-16

Cited by 7 publications

(5 citation statements)

References 45 publications

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“…Phage display has also been previously used to obtain VHH antibodies from llamas immunized with InlB, although the applicability of this nanobody in immunodetection was not further investigated ( 44 ). Binders toward other targets were described, such as the hybridoma-derived mAbs against LapB, which were able to recognize more than 46 of the 53 L. monocytogenes strains included in the experiment ( 45 ). The use of a phage display human synthetic antibody library to generate antibodies against L. monocytogenes resulted in one scFv targeting ActA found to specifically recognize 6 out of 8 tested pathogenic strains ( 46 ).…”

Section: Resultsmentioning

confidence: 99%

Phage Display-Derived Monoclonal Antibodies Against Internalins A and B Allow Specific Detection of Listeria monocytogenes

Moreira

Gronow

Dübel

et al. 2022

Front. Public Health

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Listeria monocytogenes is the causative agent of listeriosis, a highly lethal disease initiated after the ingestion of Listeria-contaminated food. This species comprises different serovars, from which 4b, 1/2a, and 1/2b cause most of the infections. Among the different proteins involved in pathogenesis, the internalins A (InlA) and B (InlB) are the best characterized, since they play a major role in the enterocyte entry of Listeria cells during early infection. Due to their covalent attachment to the cell wall and location on the bacterial surface, along with their exclusive presence in the pathogenic L. monocytogenes, these proteins are also used as detection targets for this species. Even though huge advancements were achieved in the enrichment steps for subsequent Listeria detection, few studies have focused on the improvement of the antibodies for immunodetection. In the present study, recombinant InlA and InlB produced in Escherichia coli were used as targets to generate antibodies via phage display using the human naïve antibody libraries HAL9 and HAL10. A set of five recombinant antibodies (four against InlA, and one against InlB) were produced in scFv-Fc format and tested in indirect ELISA against a panel of 19 Listeria strains (17 species; including the three main serovars of L. monocytogenes) and 16 non-Listeria species. All five antibodies were able to recognize L. monocytogenes with 100% sensitivity (CI 29.24–100.0) and specificity (CI 88.78–100.0) in all three analyzed antibody concentrations. These findings show that phage display-derived antibodies can improve the biological tools to develop better immunodiagnostics for L. monocytogenes.

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Section: Resultsmentioning

confidence: 99%

Phage Display-Derived Monoclonal Antibodies Against Internalins A and B Allow Specific Detection of Listeria monocytogenes

Moreira

Gronow

Dübel

et al. 2022

Front. Public Health

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“…Pathogenicity of the Listeria spp. could be identified by testing for the presence of a virulence factor named Listeria adhesion protein B (LapB), as noted by a recent research report [ 9 ].…”

Section: Discussionmentioning

confidence: 99%

Human Listeriosis Presenting as Breast Abscess: Report of a Rare Case

Kandi¹

2017

Cureus

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An abscess is defined as a collection of pus in various tissues of the body including skin and other organs. Abscesses most commonly are formed on the skin under the armpits, groin areas, and rectal areas. Most abscesses involve microbial infections with few remaining sterile. The treatment of abscesses includes both medical and surgical intervention. In the era of multidrug resistance, isolation and identification of the causative microbe and testing for antimicrobial susceptible patterns assume greater significance for the better management of patients, thereby reducing the resultant morbidity and mortality. Listeria spp. are a group of aerobic and non-spore forming gram-positive bacilli. They are present in the environment, soil, and water. Listeria spp. have also been noted to be present as a normal intestinal flora of animals. They are known for their ability to thrive under both cold and hot environmental conditions. Human infections with Listeria spp. have not been frequently reported, mostly because of the difficulty in laboratory identification and complex clinical presentations. In humans, Listeria spp. have been frequently responsible for food poisoning and neonatal meningitis. Although not considered as a classic pathogen, Listeria spp. are associated with infections in elderly people, pregnant women, newborns, and persons with weakened immune systems. This report presents a case of breast abscess caused by Listeria spp. in a young lactating female belonging to rural India.

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“…In another study, the expression of the surface protein LapB was studied in different serotypes of Listeria spp to develop Anti-LapB Monoclonal Antibodies for identification and detection of virulence strains [ 35 ]. Also, the regulation of virulence genes is studied in L .…”

Section: Discussionmentioning

confidence: 99%

Small molecules targeting LapB protein prevent Listeria attachment to catfish muscle

et al. 2017

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Listeria monocytogenes is a Gram-positive foodborne pathogen and the causative agent of listeriosis. L. monocytogenes lapB gene encodes a cell wall surface anchor protein, and mutation of this gene causes Listeria attenuation in mice. In this work, the potential role of Listeria LapB protein in catfish fillet attachment was investigated. To achieve this, boron-based small molecules designed to interfere with the active site of the L. monocytogenes LapB protein were developed, and their ability to prevent L. monocytogenes attachment to fish fillet was tested. Results indicated that seven out of nine different small molecules were effective in reducing the Listeria attachment to catfish fillets. Of these, three small molecules (SM3, SM5, and SM7) were highly effective in blocking Listeria attachment to catfish fillets. This study suggests an alternative strategy for reduction of L. monocytogenes contamination in fresh and frozen fish products.

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Expression of Surface Protein LapB by a Wide Spectrum of Listeria monocytogenes Serotypes as Demonstrated with Anti-LapB Monoclonal Antibodies

Cited by 7 publications

References 45 publications

Phage Display-Derived Monoclonal Antibodies Against Internalins A and B Allow Specific Detection of Listeria monocytogenes

Phage Display-Derived Monoclonal Antibodies Against Internalins A and B Allow Specific Detection of Listeria monocytogenes

Human Listeriosis Presenting as Breast Abscess: Report of a Rare Case

Small molecules targeting LapB protein prevent Listeria attachment to catfish muscle

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