Expression of stress-related genes in diapause of European corn borer (Ostrinia nubilalis Hbn.)

Popović, Željko D.; Subotić, Ana; Nikolić, Tatjana; Radojičić, R.; Blagojević, Duško; Grubor-Lajšić, Gordana; Košťál, Vladimı́r

doi:10.1016/j.cbpb.2015.04.004

Cited by 24 publications

(25 citation statements)

References 78 publications

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“…Because higher expression of Hsps occurred 7 days after RNAi, we deduce that the expressions of Hsps related to stress tolerance were not regulated by FAS2 . We also examined the expression of another two diapause-related genes involved in oxidative stress, superoxide dismutase (SOD ) and glutathione S transferase (GST )2930. Interestingly, the mRNA expression of SOD in the fat body decreased, whereas that of GST increased after dsFAS2 injection (Fig.…”

Section: Resultsmentioning

confidence: 99%

“…SOD expression was dramatically higher in diapausing C. pipiens females than in their non-diapausing counterparts29. However, GST was clearly downregulated in diapausing European corn borers Ostrinia nubilalis compared to non-diapausing controls30. This suggests that reduced lipid accumulation due to FAS2 knockdown negatively affects the expression of oxidative stress-related genes in diapause-destined female C. bowringi .…”

Section: Discussionmentioning

confidence: 97%

“…This suggests that reduced lipid accumulation due to FAS2 knockdown negatively affects the expression of oxidative stress-related genes in diapause-destined female C. bowringi . Because oxidative stress depends on the balance between pro- and anti-oxidants3042, we couldn’t make inferences on oxidative stress from transcript levels of antioxidant levels alone. Thus, more experiment is needed to clarify whether oxidative stress was increased, decreased, or unchanged regulated by decreasing lipid accumulation.…”

Section: Discussionmentioning

confidence: 99%

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Fatty acid synthase 2 contributes to diapause preparation in a beetle by regulating lipid accumulation and stress tolerance genes expression

Tan

Liu

Zhu

et al. 2017

Sci Rep

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Diapause, also known as dormancy, is a state of arrested development that allows insects to survive unfavorable environmental conditions. Diapause-destined insects store large amounts of fat when preparing for diapause. However, the extent to which these accumulated fat reserves influence diapause remains unclear. To address this question, we investigated the function of fatty acid synthase (FAS), which plays a central role in lipid synthesis, in stress tolerance, the duration of diapause preparation, and whether insects enter diapause or not. In diapause-destined adult female cabbage beetles, Colaphellus bowringi, FAS2 was more highly expressed than FAS1 at the peak stage of diapause preparation. FAS2 knockdown suppressed lipid accumulation and subsequently affected stress tolerance genes expression and water content. However, silencing FAS2 had no significant effects on the duration of diapause preparation or the incidence of diapause. FAS2 transcription was suppressed by juvenile hormone (JH) and the JH receptor methoprene-tolerant (Met). These results suggest that the absence of JH-Met induces FAS2 expression, thereby promoting lipid storage in diapause-destined female beetles. These results demonstrate that fat reserves regulate stress tolerance genes expression and water content, but have no significant effect on the duration of diapause preparation or the incidence of diapause.

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Section: Resultsmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 97%

Section: Discussionmentioning

confidence: 99%

See 1 more Smart Citation

Fatty acid synthase 2 contributes to diapause preparation in a beetle by regulating lipid accumulation and stress tolerance genes expression

Tan

Liu

Zhu

et al. 2017

Sci Rep

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“…Expression of Hsp90 in response to diapause showed either up-or down-regulated patterns, and non-responsive patterns to diapause were expressed as unchanged transcriptions. For example, Hsp90 is always up-regulated in many species during diapause, such as the rice stem borer Chilo suppressalis (Sonoda et al, 2006b), the cotton bollworm Helicoverpa armigera (Chen et al, 2010) and the European corn borer Ostrinia nubilalis (Popovic et al, 2015). However, in the flesh fly S. crassipalpis (Rinehart & Denlinger, 2000), the corn earworm H. zea (Zhang & Denlinger, 2010), O. fuscidentalis (Tungjitwitayakul et al, 2008) and Nasonia vitripennis (Wolschin & Gadau, 2009), it was down-regulated during diapause progression.…”

Section: Hsp90 Expression During Summer and Winter Diapausementioning

confidence: 99%

Molecular characterization of three Hsp90 from Pieris and expression patterns in response to cold and thermal stress in summer and winter diapause of Pieris melete

Zou

et al. 2017

Insect Science

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Heat shock proteins (Hsps) have been linked to stresses and winter diapause in insects, but whether they are components of summer diapause is still unknown. In this study, complementary DNAs of Hsp90 from Pieris melete, Pieris rapae and Pieris canidia named PmHsp90, PrHsp90 and PcHsp90, respectively, were cloned and sequenced. The deduced amino acid sequence consisted of 718 amino acid residues with a putative molecular mass of 82.6, 82.6 and 82.7 kDa, respectively. The amino acid sequences contained all of the five conserved signature motifs in the Hsp90 family and a bHLH protein folding activity region. The differential expression pattern of PmHsp90 in response to summer diapause and winter diapause, which are related to heat/cold stress, was investigated. Cold stress induced Hsp90 up-regulation in summer and winter diapause pupae, but not in non-diapause individuals. Heat shock up-regulated PmHsp90 gradually with an increase in temperature in summer diapause, and PmHsp90 was rapidly up-regulated in winter diapause. After 30 min heat shock at 39°C, substantial up-regulation of PmHsp90 transcript levels were observed both in summer and winter diapause. However, in non-diapause a relatively stable expression was found under different durations of 39°C heat shock. Compared to the optimal treatment of 18°C for diapause development, a high temperature acclimation of 31°C induced PmHsp90 up-regulation in summer diapause, whereas a low temperature acclimation of 4°C induced up-regulation in winter diapause. The current results indicate that Hsp90 may play an important role in response to heat/cold stress both in summer and winter diapause.

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“…For example, the synthesis of Hsps is up-regulated in diapause-destined embryos of A . franciscana [ 1 , 30 , 31 ] and in other organisms undergoing diapause such as rotifers [ 54 – 55 ], copepods [ 56 – 57 ] and insects [ 8 , 58 – 63 ], where they assist in maintaining protein homeostasis and stress tolerance. In spite of its importance in the regulation of molecular chaperone synthesis and stress tolerance Hsf1 function during diapause, a physiological adaptation to stress, has received limited attention.…”

Section: Discussionmentioning

confidence: 99%

Stress tolerance in diapausing embryos of Artemia franciscana is dependent on heat shock factor 1 (Hsf1)

Tan

MacRae

2018

PLoS ONE

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Embryos of the crustacean, Artemia franciscana, may undergo oviparous development, forming encysted embryos (cysts) that are released from females and enter diapause, a state of suppressed metabolism and greatly enhanced stress tolerance. Diapause-destined embryos of A. franciscana synthesize three small heat shock proteins (sHsps), p26, ArHsp21 and ArHsp22, as well as artemin, a ferritin homologue, all lacking in embryos that develop directly into nauplii. Of these diapause-specific molecular chaperones, p26 and artemin are important contributors to the extraordinary stress tolerance of A. franciscana cysts, but how their synthesis is regulated is unknown. To address this issue, a cDNA for heat shock factor 1 (Hsf1), shown to encode a protein similar to Hsf1 from other organisms, was cloned from A. franciscana. Hsf1 was knocked down by RNA interference (RNAi) in nauplii and cysts of A. franciscana. Nauplii lacking Hsf1 died prematurely upon release from females, showing that this transcription factor is essential to the survival of nauplii. Diapause cysts with diminished amounts of Hsf1 were significantly less stress tolerant than cysts containing normal levels of Hsf1. Moreover, cysts deficient in Hsf1 possessed reduced amounts of p26, ArHsp21, ArHsp22 and artemin, revealing dependence on Hsf1 for expression of their genes and maximum stress tolerance. The results demonstrate an important role for Hsf1, likely in concert with other transcription factors, in the survival and growth of A. franciscana and in the developmentally regulated synthesis of proteins responsible for the stress tolerance of diapausing A. franciscana cysts.

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Expression of stress-related genes in diapause of European corn borer (Ostrinia nubilalis Hbn.)

Cited by 24 publications

References 78 publications

Fatty acid synthase 2 contributes to diapause preparation in a beetle by regulating lipid accumulation and stress tolerance genes expression

Fatty acid synthase 2 contributes to diapause preparation in a beetle by regulating lipid accumulation and stress tolerance genes expression

Molecular characterization of three Hsp90 from Pieris and expression patterns in response to cold and thermal stress in summer and winter diapause of Pieris melete

Stress tolerance in diapausing embryos of Artemia franciscana is dependent on heat shock factor 1 (Hsf1)

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