Expression of Steroidogenic Acute Regulatory Protein in the Human Corpus Luteum throughout the Luteal Phase

Devoto, Luigi; Kohen, Paulina; González, Ricardo R.; Castro, Olga; Retamales, Iván; Vega, Margarita; Carvallo, Pilar; Christenson, Lane K.; Strauss, Jerome F.

doi:10.1210/jcem.86.11.7982

Cited by 36 publications

(24 citation statements)

References 34 publications

(36 reference statements)

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…It is interesting that the levels of P4 before the LH surge in the FF were extremely high considering that the steroidogenic machinery in granulosa cells before the LH surge is limited. The steroidogenic acute regulatory protein (StAR) that is critical for P4 production is scarcely expressed in GCs of preovulatory follicles (23)(24)(25). These data suggest that the contribution of theca steroidogenesis may be critical to the follicular P4 during the pre-LH period.…”

Section: Discussionmentioning

confidence: 99%

Transient expression of progesterone receptor and cathepsin-l in human granulosa cells during the periovulatory period

García

Kohen

Maldonado

et al. 2012

Fertility and Sterility

Self Cite

View full text Add to dashboard Cite

Section: Discussionmentioning

confidence: 99%

Transient expression of progesterone receptor and cathepsin-l in human granulosa cells during the periovulatory period

García

Kohen

Maldonado

et al. 2012

Fertility and Sterility

Self Cite

View full text Add to dashboard Cite

“…Indeed, there is evidence that NO decreases steroidogenesis although in different experimental schemes (Olson et al 1996, Masuda et al 1997, Vega et al 2000. The mechanisms involved may be related to the inhibition produced by the NO of the activity of steroid synthesislimiting enzyme, cytochrome P 450 , of the two isoforms of adenylyl cyclase, the second intracellular messenger (McVey et al 1999), and/or of the inhibition of the steroidogenic acute regulatory protein (StAR) protein (Devoto et al 2001). In our case it should be emphasized that the terminals of the ovarian nervous plexus reach the steroidogenic cells where the inducible NOS or eNOS isoenzymes have been found (Dunnam et al 1999).…”

Section: Discussionmentioning

confidence: 99%

The cholinergic influence on the mesenteric ganglion affects the liberation of ovarian steroids and nitric oxide in oestrus day rats: characterization of an ex vivo system

Orozco¹,

Sosa²,

Fillipa³

et al. 2006

Journal of Endocrinology

View full text Add to dashboard Cite

The axons that constitute the ovarian nervous plexus originate mostly in the principal neurons of the superior mesenteric ganglion (SMG) that is part of the sympathetic ganglionic chain and exhibits cholinergic receptors. In order to observe the effect of acetylcholine, the main neurotransmitter in the ganglionic transmission, the purpose of the present work was: first, to standardize an integrated ex vivo superior mesenteric ganglion-ovarian nervous plexus-ovary (SMG-ONP-O) system in oestrus day rats; secondly, to determine if the ganglionic cholinergic stimulus modifies the release of nitric oxide and steroids in the ovary compartment in the absence of humoral factors; and thirdly, to investigate if there are differences in the responses between the left and right ovaries caused by the neural stimulus. The ex vivo experimental left and right systems were developed and standardized. The systems were incubated in Krebs-Ringer phosphate buffer in a Dubnoff metabolic shaker. The progesterone release was determined to standardize the incubation times, obtaining different responses between the left and right systems, which shows that both systems have their own autonomic tone. Non-specific stimulation with KCl in the ganglion compartment provoked different responses in terms of release of progesterone and oestradiol. Progesterone decreased in the left and right systems.However, oestradiol diminished at short times and increased at 60 and 120 min in the left ovary, whereas it increases at 30 and 60 min in the right ovary. These different responses show the sensitivity and viability of both systems. When acetylcholine was used in the ganglion compartment, the release of nitric oxide, progesterone, androstenedione and oestradiol was evaluated. The liberation of nitrite increased at 15, 30 and 60 min in the left system and decreased in the right system at 120 min. Progesterone showed a decrease in its release at 15, 30 and 120 min and androstenedione at 15 min in the left ovary compartment. In the right ovary, only progesterone decreased in relation to the control at 120 min while androstenedione did not show significant changes. Oestradiol showed an increase in the left ovary compartment at all the studied times, while in the right ovary it did not show any changes. These results indicate that the neural stimulus from the superior mesenteric ganglion through the ovarian nervous plexus is one of the factors modulating the secretory activity of the ovarian steroids and nitric oxide. The system is viable and also shows a different sensitivity of the left ovary in relation to the right one at least in this cycle stage, characterized by marked irrigation and profound structural changes in the ovary.

show abstract

“…Then, 25 μg of protein extract was run on a 10% SDS-polyacrylamide gel and transferred to a polyvinylidene difluoride membrane (Immobilon, Millipore Corporation, Billerica, MA) by electroblotting overnight. The membrane was washed in 1× PBS/0.3% Tween-20, blocked with buffer containing 1% BSA and 5% milk, and incubated with an anti-steroidogenic acute regulatory protein (StAR) antibody at a concentration of 1:200 (Devoto et al, 2001) for 1.5 h at room temperature. The blot was then washed with buffer, and a secondary antibody of horseradish peroxidase-conjugated donkey antirabbit IgG (Amersham Biosciences, Piscataway, NJ) at 1:1000 diluted in 1× PBS/0.3% Tween-20/1% BSA/5% milk was incubated for 1.5 h at room temperature.…”

Section: Immunoblot Analysismentioning

confidence: 99%

The Circadian Clock Protein BMAL1 Is Necessary for Fertility and Proper Testosterone Production in Mice

et al. 2008

View full text Add to dashboard Cite

Although it is well established that the circadian clock regulates mammalian reproductive physiology, the molecular mechanisms by which this regulation occurs are not clear. The authors investigated the reproductive capacity of mice lacking Bmal1 (Arntl, Mop3), one of the central circadian clock genes. They found that both male and female Bmal1 knockout (KO) mice are infertile. Gross and microscopic inspection of the reproductive anatomy of both sexes suggested deficiencies in steroidogenesis. Male Bmal1 KO mice had low testosterone and high luteinizing hormone serum concentrations, suggesting a defect in testicular Leydig cells. Importantly, Leydig cells rhythmically express BMAL1 protein, suggesting peripheral control of testosterone production by this clock protein. Expression of steroidogenic genes was reduced in testes and other steroidogenic tissues of Bmal1 KO mice. In particular, expression of the steroidogenic acute regulatory protein (StAR) gene and protein, which regulates the rate-limiting step of steroidogenesis, was decreased in testes from Bmal1 KO mice. A direct effect of BMAL1 on StAR expression in Leydig cells was indicated by in vitro experiments showing enhancement of StAR transcription by BMAL1. Other hormonal defects in male Bmal1 KO mice suggest that BMAL1 also has functions in reproductive physiology outside of the testis. These results enhance understanding of how the circadian clock regulates reproduction.Keywords circadian rhythms; fertility; testosterone; testes; sperm; StAR; mice Disruption of circadian rhythms results in a variety of pathophysiologic states (Hastings et al., 2003). Reproductive physiology, in particular, is profoundly influenced by circadian rhythms (Boden and Kennaway, 2006). In various insect species, the circadian clock is necessary for proper ovulation, sperm production, and fertility (Giebultowicz et al., 1989;Beaver et al., 2002;Beaver et al., 2003;Beaver and Giebultowicz, 2004 (Lucas and Eleftheriou, 1980;Clair et al., 1985;Chappell et al., 2003;Miller et al., 2004). For example, the surge of luteinizing hormone (LH) necessary for ovulation in rodents, which occurs at the same time of day during each estrous cycle, requires a functional circadian clock (Barbacka-Surowiak et al., 2003). In addition, at the onset of puberty, a clear diurnal rhythm of gonadotropin serum levels is established in both mice and humans (Andrews and Ojeda, 1981;Jean-Faucher et al., 1986;Dunkel et al., 1992;Apter et al., 1993). It is unclear whether this diurnal rhythm continues into adulthood, but testosterone serum concentration shows daily oscillations in adult male mice and humans (Lucas and Eleftheriou, 1980;Clair et al., 1985). Although the association between circadian rhythms and testosterone is a long-established phenomenon, the molecular mechanisms by which the circadian clock regulates testosterone production are unknown.The circadian clock is based on a transcription translation feedback loop that results in the cyclic expression of genes and proteins over a 24-h p...

show abstract

Expression of Steroidogenic Acute Regulatory Protein in the Human Corpus Luteum throughout the Luteal Phase

Cited by 36 publications

References 34 publications

Transient expression of progesterone receptor and cathepsin-l in human granulosa cells during the periovulatory period

Transient expression of progesterone receptor and cathepsin-l in human granulosa cells during the periovulatory period

The cholinergic influence on the mesenteric ganglion affects the liberation of ovarian steroids and nitric oxide in oestrus day rats: characterization of an ex vivo system

The Circadian Clock Protein BMAL1 Is Necessary for Fertility and Proper Testosterone Production in Mice

Contact Info

Product

Resources

About