Expression of steroid receptor coactivators and corepressors in human endometrial hyperplasia and carcinoma with relevance to steroid receptors and Ki‐67 expression

Abstract

Thin palladium membranes of different thicknesses were prepared on sol‐gel derived mesoporous γ‐alumina/α‐alumina and yttria‐stabilized zirconia/α‐alumina supports by a method combining sputter deposition and electroless plating. The effect of metal‐support interface on hydrogen transport permeation properties was investigated by comparing hydrogen permeation data for these membranes measured under different conditions. Hydrogen permeation fluxes for the Pd/γ‐Al2O3/α‐Al2O3 membranes are significantly smaller than those for the Pd/YSZ/α‐Al2O3 membranes under similar conditions. As the palladium membrane thickness increases, the difference in permeation fluxes between these two groups of membranes decreases and the pressure exponent for permeation flux approaches 0.5 from 1. Analysis of the permeation data with a permeation model shows that both groups of membranes have similar hydrogen permeability for bulk diffusion, but the Pd/γ‐Al2O3/α‐Al2O3 membranes exhibit a much lower surface reaction rate constant with higher activation energy, due possibly to the formation of Pd‐Al alloy, than the Pd/YSZ/α‐Al2O3 membranes. © 2009 American Institute of Chemical Engineers AIChE J, 2009

Steroid receptor coactivator AIB1 in endometrial carcinoma, hyperplasia and normal endometrium: correlation with clinicopathologic parameters and biomarkers

“…23 An additional study examined SRC-1, p300/CBP, and the co-repressors N-CoR and SMRT, but not AIB1, and found that both SRC-1 and p300/ CBP were reduced in endometrial carcinoma as compared with normal endometrium and hyperplastic endometrium. 25 In contrast, we observe no change in SRC-1 and CBP/p300 in endometrial carcinoma or carcinoma-associated complex atypical hyperplasia vs the carcinoma-associated normal endometrium. At the mRNA transcript level, SRC-1, 2 and 3 and the co-repressors N-CoR and SMRT were measured in endometrial carcinoma and all were upregulated in malignant endometrium.…”

“…23,25 However, we did not find a significant correlation between AIB1 and total PR or PR-B. It is interesting that AIB1 correlates with ER, but not with PR.…”

Gain of OGP, an Estrogen-Regulated Oviduct-Specific Glycoprotein, Is Associated with the Development of Endometrial Hyperplasia and Endometrial Cancer

“…This may be due to variations in the relative levels of ER coactivators or corepressors in endometrial epithelium compared with oviductal epithelium (32). However, a recent study showed that the expression of the coactivators, SRC-1 and p300/CBP, decreased in endometrial hyperplasia, whereas the corepressor, NcoR, was elevated (33). Despite the lower levels of coactivators and higher levels of the corepressor, Uchikawa et al (33) showed a topologic correlation between the expression of ER and SRC-1, suggesting that hyperplasias remain responsive to estrogen.…”

“…However, a recent study showed that the expression of the coactivators, SRC-1 and p300/CBP, decreased in endometrial hyperplasia, whereas the corepressor, NcoR, was elevated (33). Despite the lower levels of coactivators and higher levels of the corepressor, Uchikawa et al (33) showed a topologic correlation between the expression of ER and SRC-1, suggesting that hyperplasias remain responsive to estrogen. On the basis of these findings, we propose that it may be the exposure to prolonged estrogen that is stimulating the ectopic expression of oviduct-specific glycoprotein in hyperplasias.…”

Molecular and Cellular Mechanisms for Differentiation and Regeneration of the Uterine Endometrium

“…Coactivators and Corepressors cAMP activation of the PKA pathway disrupts the interaction of the PR with the corepressors NCoR and SMRT [59], thereby facilitating recruitment of the coactivator SRC-1 [60]. These corepressors and SRC-1 are all present in the human endometrium [61]; however, how cAMP regulates their behavior remains to be elucidated.…”