Expression of stathmin family genes in human tissues: non-neural-restricted expression for SCLIP

Bièche, Ivan; Maucuer, Alexandre; Laurendeau, Ingrid; Lachkar, Sylvie; Spano, Anthony; Frankfurter, Anthony; Lévy, Pascale; Manceau, Valérie; Sobel, André; Vidaud, Michel; Curmi, Patrick A.

doi:10.1016/s0888-7543(03)00031-4

Cited by 56 publications

(48 citation statements)

References 45 publications

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“…Our data from histological and cytological detection suggested that stathmin expression was up-regulated with the progression of hepatocarcinogenesis. It is reported that stathmin is abundantly expressed in fetal liver, on the contrary, dramatically decreased in adult liver (19). In our study, stathmin expression was negative in normal liver tissue, low in hepatitis and hepatic cirrhosis tissues and significantly up-regulated in HCC tissue.…”

Section: Discussioncontrasting

confidence: 43%

Up-regulated expression of stathmin may be associated with hepatocarcinogenesis

Gan

Guo²,

Li³

et al. 2010

Oncol Rep

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Abstract. Stathmin is a microtubule-destabilizing protein ubiquitously expressed in vertebrates and overexpressed in a variety of human malignancies. Down-regulation of its expression will contribute to optimize therapeutic outcomes in the treatment of these malignancies. This research aimed to demonstrate effects of stathmin expression silencing on hepatocellular carcinoma (HCC) cell proliferation, apoptosis, cell adhesion and motility behavior in vitro and further reveal significance of stathmin expression in tissues associated with hepatocarcinogenesis. The expression of stathmin in normal liver, hepatitis, cirrhosis and HCC tissues was detected by immunohistochemistry analysis (IHC), stathmin expression was inhibited in an HCC cell line-HCCLM3 with high metastatic potential by small interfering RNAs (siRNAs). After transfection with siRNAs, HCCLM3 cells proliferation was detected by CCK-8 (cell count kit), cell apoptosis was analyzed by FACS, cell adhesion was investigated by cell adhesion assay and motility ability was demonstrated by in vitro migration and invasion assays. Stathmin expression was up-regulated in HCC tissues, especially in metastatic HCC tissues, compared with normal liver, hepatitis and hepatic cirrhosis tissues. Expression of stathmin in HCCLM3 cells was efficiently inhibited by specific siRNAs. Silencing of stathmin expression obviously suppressed HCCLM3 cell proliferation and markedly induced cell apoptosis. Moreover, defect of stathmin expression in HCCLM3 cell inhibited cell adhesion, restrained cell migration and repressed invasion. Stathmin expression correlates with hepatocarcinogenesis and tumor progression. This molecule may be a promising therapeutic target in patients with hepatocellular carcinoma.

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Section: Discussioncontrasting

confidence: 43%

Up-regulated expression of stathmin may be associated with hepatocarcinogenesis

Gan

Guo²,

Li³

et al. 2010

Oncol Rep

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“…As such, stathmin is widely expressed in many cells, especially in those engaging in proliferation and/or migration. Intriguingly, however, the strikingly dominant expression of stathmin in mammalian brains and spinal cord, and especially in fetal brains (26), suggests a special relevance to the CNS, unexplained by its traditional and ubiquitous role as a regulator of microtubules. This applies even more strongly to the three other family members of stathmin, the homologs superior cervical ganglion 10 protein (SCG10; stathmin-2), SCG10-like protein (SCLIP; stathmin-3), and RB3 (stathmin-4), which are highly conserved among vertebrates.…”

Section: Figurementioning

confidence: 99%

“…This applies even more strongly to the three other family members of stathmin, the homologs superior cervical ganglion 10 protein (SCG10; stathmin-2), SCG10-like protein (SCLIP; stathmin-3), and RB3 (stathmin-4), which are highly conserved among vertebrates. For example, expression levels of SCLIP and RB3 in the CNS are $100 times greater than in other human tissues (26). After brain trauma, stathmin levels increase (19,20), as they do during axonal and dendritic growth (21,27).…”

Section: Figurementioning

confidence: 99%

The Microtubule Regulator Stathmin Is an Endogenous Protein Agonist for TLR3

Bsibsi

Bajramović

Vogt

et al. 2010

The Journal of Immunology

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TLR3 recognizes dsRNAs and is considered of key importance to antiviral host-defense responses. TLR3 also triggers neuroprotective responses in astrocytes and controls the growth of axons and neuronal progenitor cells, suggesting additional roles for TLR3-mediated signaling in the CNS. This prompted us to search for alternative, CNS-borne protein agonists for TLR3. A genome-scale functional screening of a transcript library from brain tumors revealed that the microtubule regulator stathmin is an activator of TLR3-dependent signaling in astrocytes, inducing the same set of neuroprotective factors as the known TLR3 agonist polyinosinic:polycytidylic acid. This activity of stathmin crucially depends on a long, negatively charged α helix in the protein. Colocalization of stathmin with TLR3 on astrocytes, microglia, and neurons in multiple sclerosis-affected human brain indicates that as an endogenous TLR3 agonist, stathmin may fulfill previously unsuspected regulatory roles during inflammation and repair in the adult CNS.

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“…These include genes that have been associated with regulation of the cell cycle and cell proliferation including YB-1 and stathmin (Mori and Morii, 2002;Kuwano et al, 2003;Bieche et al, 2003). Intriguingly, two of the differentially expressed cDNAs are RING finger proteins which in many systems have been shown to function as ubiquitin-protein ligases and to mediate major developmental events including Notch activation, by regulating cell surface levels of Delta expression and the generation of anterior ectoderm (Deblandre et al, 2001;Borchers et al, 2002;Hatakeyama and Nakayama, 2003).…”

Section: Discussionmentioning

confidence: 99%

Identification of genes regulating sensory neuron genesis and differentiation in the avian dorsal root ganglia

Nelson

Sadhu

Kasemeier

et al. 2004

Developmental Dynamics

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The dorsal root ganglia (DRG) derive from a population of migrating neural crest cells that coalesce laterally to the neural tube. As the DRG matures, discrete cell types emerge from a pool of differentiating progenitor cells. To identify genes that regulate sensory genesis and differentiation, we have designed screens to identify members from families of known regulatory molecules such as receptor tyrosine kinases, and generated full-length and subtractive cDNA libraries between immature and mature DRG for identifying novel genes not previously implicated in DRG development. Several genes were identified in these analyses that belong to important regulatory gene families. Quantitative PCR confirmed differential expression of candidate cDNAs identified from the subtraction/differential screening. In situ hybridization further validated dynamic expression of several cDNAs identified in our screens. Our results demonstrate the utility of combining specific and general screening approaches for isolating key regulatory genes involved in the genesis and differentiation of discrete cell types and tissues within the classic embryonic chick model system. Developmental Dynamics 229:618 -629, 2004.

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Expression of stathmin family genes in human tissues: non-neural-restricted expression for SCLIP

Cited by 56 publications

References 45 publications

Up-regulated expression of stathmin may be associated with hepatocarcinogenesis

Up-regulated expression of stathmin may be associated with hepatocarcinogenesis

The Microtubule Regulator Stathmin Is an Endogenous Protein Agonist for TLR3

Identification of genes regulating sensory neuron genesis and differentiation in the avian dorsal root ganglia

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