Expression of Specific Markers and Particle Transport in a New Human Intestinal M-Cell Model

Gullberg, Elisabet; Leonard, Maurice; Karlsson, Johan; Hopkins, Ann M.; Brayden, David J.; Baird, Alan W.; Artursson, Per

doi:10.1006/bbrc.2000.4038

Cited by 240 publications

(125 citation statements)

References 19 publications

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“…The basolateral side of the insert is filled with 1.5 ml of complete growth medium. At 14 days after differentiation, 5 ϫ 10 5 Raji B lymphocytes were added to the basolateral chamber in 1 ml of complete growth medium and maintained for 6 days (30,31). The corresponding monocultures were used as a model for normal enterocytes.…”

Section: Methodsmentioning

confidence: 99%

The IbeA Invasin of Adherent-Invasive Escherichia coli Mediates Interaction with Intestinal Epithelia and Macrophages

Cieza

Ross

et al. 2015

Infect Immun

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Section: Methodsmentioning

confidence: 99%

The IbeA Invasin of Adherent-Invasive Escherichia coli Mediates Interaction with Intestinal Epithelia and Macrophages

Cieza

Ross

et al. 2015

Infect Immun

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“…Apart from goblet cells and enterocytes, M cells are also suggested to be responsible for the transport of nanoparticles. Based on a co-culture model of Peyer's patch lymphocytes (mouse and human Raji B) and Caco-2 cells (Gullberg et al 2000;Kerneis et al 1997), an advanced triple-culture model that also integrates M cells has been developed (Antunes et al 2013;Mahler et al 2012;Schimpel et al 2014). Mahler et al studied the impact of polystyrene particles on iron absorption and verified reliability of the model with an intestinal chicken loop in vivo model.…”

Section: Models For the Small Intestinementioning

confidence: 99%

Oral uptake of nanoparticles: human relevance and the role of in vitro systems

Frőhlich

Roblegg

2016

Arch Toxicol

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Nanoparticles (NPs) present in environment, consumer and health products, food and medical applications lead to a high degree of human exposure and concerns about potential adverse effects on human health. For the general population, the exposure through contact with the skin, inhalation and oral uptake are most relevant. Since in vivo testing is only partly able to study the effects of human oral exposure, physiologically relevant in vitro systems are being developed. This review compared the three routes taking into account the estimated concentration, size of the exposed area, morphology of the involved barrier and translocation rate. The high amounts of NPs in food, the large absorption area and the relatively high translocation rate identified oral uptake as most important portal of entry for NPs into the body. Changes of NP properties in the physiological fluids, mechanisms to cross mucus and epithelial barrier, and important issues in the use of laboratory animals for oral exposure are mentioned. The ability of in vitro models to address the varying conditions along the oro-gastrointestinal tract is discussed, and requirements for physiologically relevant in vitro testing of orally ingested NPs are listed.

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“…[194] Gullberg et al established an in vitro co-culture model of human intestinal epithelial Caco-2 cells and B-cell lymphoma Raji cells for the expression, investigation, and development of the M-cell phenotype in a controlled in vitro manner. [195] This co-culture model showed that intercellular communication resulted in altered surface receptor expression patterns and increased transcytotic rates compared to monoculture. [195] Evaluating in vitro targeting results using co-culture systems of this type may be easier to translate to in vivo.…”

Section: Heterogeneous Cell Culture Modelsmentioning

confidence: 98%

“…[195] This co-culture model showed that intercellular communication resulted in altered surface receptor expression patterns and increased transcytotic rates compared to monoculture. [195] Evaluating in vitro targeting results using co-culture systems of this type may be easier to translate to in vivo. [196] Moreover, with regard to cancer metastasis, the influence of intercellular communication is important.…”

Section: Heterogeneous Cell Culture Modelsmentioning

confidence: 98%

Particle Targeting in Complex Biological Media

Dai

Bertleff‐Zieschang

Braunger

et al. 2017

Adv Healthcare Materials

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Over the past few decades, nanoengineered particles have gained increasing interest for applications in the biomedical realm, including diagnosis, imaging, and therapy. When functionalized with targeting ligands, these particles have the potential to interact with specific cells and tissues, and accumulate at desired target sites, reducing side effects and improve overall efficacy in applications such as vaccination and drug delivery. However, when targeted particles enter a complex biological environment, the adsorption of biomolecules and the formation of a surface coating (e.g., a protein corona) changes the properties of the carriers and can render their behavior unpredictable. For this reason, it is of importance to consider the potential challenges imposed by the biological environment at the early stages of particle design. This review describes parameters that affect the targeting ability of particulate drug carriers, with an emphasis on the effect of the protein corona. We highlight strategies for exploiting the protein corona to improve the targeting ability of particles. Finally, we provide suggestions for complementing current in vitro assays used for the evaluation of targeting and carrier efficacy with new and emerging techniques (e.g., 3D models and flow‐based technologies) to advance fundamental understanding in bio‐nano science and to accelerate the development of targeted particles for biomedical applications.

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Expression of Specific Markers and Particle Transport in a New Human Intestinal M-Cell Model

Cited by 240 publications

References 19 publications

The IbeA Invasin of Adherent-Invasive Escherichia coli Mediates Interaction with Intestinal Epithelia and Macrophages

The IbeA Invasin of Adherent-Invasive Escherichia coli Mediates Interaction with Intestinal Epithelia and Macrophages

Oral uptake of nanoparticles: human relevance and the role of in vitro systems

Particle Targeting in Complex Biological Media

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