Expression of somatostatin and somatostatin receptor subtypes in Apolipoprotein D (ApoD) knockout mouse brain: An immunohistochemical analysis

Rajput, Padmesh S.; Billová, Sabina; Patel, Seema; Kharmate, Geetanjali; Somvanshi, Rishi K.; Kumar, Ujendra

doi:10.1016/j.jchemneu.2009.05.004

Cited by 13 publications

(18 citation statements)

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“…regionally-selective changes in somatostatin and somatostatin receptors (Rajput et al, 2009). These findings are of relevance to our study as it has been shown that somatostatin can regulate glutamate release (Grilli et al, 2004).…”

Section: Discussionsupporting

confidence: 77%

Decreased kainate receptors in the hippocampus of apolipoprotein D knockout mice

Boer

Sánchez

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et al. 2010

Progress in Neuro-Psychopharmacology and Biological Psychiatry

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Section: Discussionsupporting

confidence: 77%

Decreased kainate receptors in the hippocampus of apolipoprotein D knockout mice

Boer

Sánchez

Reinieren

et al. 2010

Progress in Neuro-Psychopharmacology and Biological Psychiatry

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“…Co-immunoprecipitation (Co-IP) in tissue extract prepared from brain regions and cell lysate prepared from HEK-293 cells was accomplished as described previously [43], [44]. Briefly, the brain tissue was homogenized in homogenization buffer and 250 µg of tissue protein was solubilized in 1 ml binding buffer (50 mM HEPES, 2 mM CaCl 2 , 5 mM MgCl 2 , pH 7.5) followed by treatment with SSTR4 specific agonist L-803087 (10 nM), δOR specific agonist SB-205607 (10 nM) alone or in combination for 30 min at 37°C.…”

Section: Methodsmentioning

confidence: 99%

δ-Opioid Receptor and Somatostatin Receptor-4 Heterodimerization: Possible Implications in Modulation of Pain Associated Signaling

Somvanshi

Kumar

2014

PLoS ONE

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Pain relief is the principal action of opioids. Somatostatin (SST), a growth hormone inhibitory peptide is also known to alleviate pain even in cases when opioids fail. Recent studies have shown that mice are prone to sustained pain and devoid of analgesic effect in the absence of somatostatin receptor 4 (SSTR4). In the present study, using brain slices, cultured neurons and HEK-293 cells, we showed that SSTR4 and δ-Opioid receptor (δOR) exist in a heteromeric complex and function in synergistic manner. SSTR4 and δOR co-expressed in cortical/striatal brain regions and spinal cord. Using cultured neuronal cells, we describe the heterogeneous complex formation of SSTR4 and δOR at neuronal cell body and processes. Cotransfected cells display inhibition of cAMP/PKA and co-activation of SSTR4 and δOR oppose receptor trafficking induced by individual receptor activation. Furthermore, downstream signaling pathways either associated with withdrawal or pain relief are modulated synergistically with a predominant role of SSTR4. Inhibition of cAMP/PKA and activation of ERK1/2 are the possible cellular adaptations to prevent withdrawal induced by chronic morphine use. Our results reveal direct intra-membrane interaction between SSTR4 and δOR and provide insights for the molecular mechanism for the anti-nociceptive property of SST in combination with opioids as a potential therapeutic approach to avoid undesirable withdrawal symptoms.

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“…In our recent study, we have shown the expression of SSTRs in both MSNs and interneurons [45]. We have recently shown the receptor specific changes in cortical brain region of SSTR5 −/− mice [46].…”

Section: Resultsmentioning

confidence: 90%

“…Rabbit polyclonal antibodies for SSTR subtypes have been previously characterized [42], [45], [73]. Mouse monoclonal antibodies for DARPP-32 and calcineurin were purchased from BD Biosciences (Mississauga, ON, Canada).…”

Section: Methodsmentioning

confidence: 99%

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Somatostatin Receptor 1 and 5 Double Knockout Mice Mimic Neurochemical Changes of Huntington's Disease Transgenic Mice

et al. 2011

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BackgroundSelective degeneration of medium spiny neurons and preservation of medium sized aspiny interneurons in striatum has been implicated in excitotoxicity and pathophysiology of Huntington's disease (HD). However, the molecular mechanism for the selective sparing of medium sized aspiny neurons and vulnerability of projection neurons is still elusive. The pathological characteristic of HD is an extensive reduction of the striatal mass, affecting caudate putamen. Somatostatin (SST) positive neurons are selectively spared in HD and Quinolinic acid/N-methyl-D-aspartic acid induced excitotoxicity, mimic the model of HD. SST plays neuroprotective role in excitotoxicity and the biological effects of SST are mediated by five somatostatin receptor subtypes (SSTR1-5).Methods and FindingsTo delineate subtype selective biological responses we have here investigated changes in SSTR1 and 5 double knockout mice brain and compared with HD transgenic mouse model (R6/2). Our study revealed significant loss of dopamine and cAMP regulated phosphoprotein of 32 kDa (DARPP-32) and comparable changes in SST, N-methyl-D-aspartic acid receptors subtypes, calbindin and brain nitric oxide synthase expression as well as in key signaling proteins including calpain, phospho-extracellular-signal-regulated kinases1/2, synapsin-IIa, protein kinase C-α and calcineurin in SSTR1/5−/− and R6/2 mice. Conversely, the expression of somatostatin receptor subtypes, enkephalin and phosphatidylinositol 3-kinases were strain specific. SSTR1/5 appears to be important in regulating NMDARs, DARPP-32 and signaling molecules in similar fashion as seen in HD transgenic mice.ConclusionsThis is the first comprehensive description of disease related changes upon ablation of G- protein coupled receptor gene. Our results indicate that SST and SSTRs might play an important role in regulation of neurodegeneration and targeting this pathway can provide a novel insight in understanding the pathophysiology of Huntington's disease.

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Expression of somatostatin and somatostatin receptor subtypes in Apolipoprotein D (ApoD) knockout mouse brain: An immunohistochemical analysis

Cited by 13 publications

References 93 publications

Decreased kainate receptors in the hippocampus of apolipoprotein D knockout mice

Decreased kainate receptors in the hippocampus of apolipoprotein D knockout mice

δ-Opioid Receptor and Somatostatin Receptor-4 Heterodimerization: Possible Implications in Modulation of Pain Associated Signaling

Somatostatin Receptor 1 and 5 Double Knockout Mice Mimic Neurochemical Changes of Huntington's Disease Transgenic Mice

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