Expression of somatic embryogenesis-related genes in sugarcane (Saccharum officinarum L.)

Maulidiya, Ahdatu Uli Khikamil; Sugiharto, Bambang; Dewanti, Parawita; Handoyo, Tri

doi:10.1007/s12892-020-00024-x

Cited by 16 publications

(8 citation statements)

References 53 publications

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“…5 a). Our results for callogenesis induction are in line with previous reports for other monocot species such as agave, sugar cane, coconut, sorghum and desho; very high doses of 2,4-D considerably inhibited callus induction 45 , 53 – 56 .…”

Section: Resultssupporting

confidence: 92%

Indirect organogenesis for high frequency shoot regeneration of two cultivars of Sansevieria trifasciata Prain differing in fiber production

García-Hernández

Loera-Quezada

Morán-Velázquez

et al. 2022

Sci Rep

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Sansevieria trifasciata is used as an indoor plant, in traditional medicine and as a fiber source. Here we characterized fibers of two of varieties of S. trifasciata, Lorentii and Hahnii, and report a protocol for their propagation based on indirect shoot organogenesis. Structural and ribbon fibers were scattered within leaf parenchyma when viewed with confocal laser scanning microscopy. Chemical analysis of the fibers by mass spectrometry and high-performance chromatography revealed higher contents of cellulose and xylose in Lorentii than in Hahnii and significant differences for total lignin between both. A protocol for de novo shoot production was then developed using leaf explants. Time-course histological analyses showed that the first events of transdifferentiation were triggered preferentially in cells surrounding fibers and vascular bundles. Callogenesis and shoot performances were quantified for both varieties, and 2,4-D at 2 and 3 mg·L-1 yielded the best results for primary calli induction and fresh calli mass. The length, number, and mass of shoots produced did not differ significantly between the two cultivars. The fast morphogenic response of S. trifasciata to in vitro culture may be useful for mass propagation or other biotechnological purposes such as metabolite production.

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Section: Resultssupporting

confidence: 92%

Indirect organogenesis for high frequency shoot regeneration of two cultivars of Sansevieria trifasciata Prain differing in fiber production

García-Hernández

Loera-Quezada

Morán-Velázquez

et al. 2022

Sci Rep

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“…It has been reported that as the formulation of the medium is modi ed, different types of callus are induced, which are classi ed based on their macroscopic characteristics (Ikeuchi et al 2013). As starting point, the type of induced callus could be originated by speci c and distinct gene expression pro le; which in turn, could be related to the interaction between growth regulators present in the culture medium and those produced by the explant itself (Iwase et al 2011;Maulidiya et al 2020).…”

Section: Resultsmentioning

confidence: 99%

“…For this purpose, some tissue culture techniques have been used to develop and improve protocols for in vitro propagation of some sugarcane varieties, either through embryogenesis and/or organogenesis (Ramanand et al 2006; Kumar and Sahoo 2009; Kavita et al 2016; Salokhe 2021); nevertheless, previous reports indicate that some sugarcane genotypes are more prone to in vitro instability than others, possibly due to the interaction between genotype and culture medium. Thus, the establishment of a suitable culture medium is critical for in vitro propagation of sugarcane, especially when it is desirable that the protocol is based on somatic embryogenesis (Snyman et al 2011;Maulidiya et al 2020).…”

Section: Introductionmentioning

confidence: 99%

Use of Response Surface Methodology on Medium Components Optimization for Embryogenic Callus Induction in Sugarcane (Saccharum Sp.) Cv. Cp 72-2086

Germán-Báez¹,

Santos-Ballardo²,

Esparza-Araiza³

et al. 2021

Preprint

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The importance of sugarcane culture has in the agriculture and industrial market, demand the development of efficient in vitro propagation systems; in this sense embryogenic callus has a great impact in basic research and industrial applications, due to this, the development of protocols for embryogenic callus induction is of great importance. In this work, an optimized protocol for embryogenic callus induction was developed; to do this, the balance of the phytohormones 2,4-dichlorophenoxyacetic acid (2,4-D) and indole-3-butyric acid (IBA) in the culture media composition was optimized using response surface methodology (RSM) in order to maximize the percentage of samples with embryogenic callus (%SEC) and the weight gain on callus produced (ΔCW) in Saccharum sp. cv. CP72-2086. Best results in the %SEC were obtained as the 2,4-D concentration decreased; meanwhile, in the case of IBA the best results were obtained in the concentration range from 2.5 to 5.5 mg L-1. Regarding ΔCW, maximum values were obtained at 2,4-D concentrations in the range of 0.5 mg L-1 to 1.89 mg L-1 in combination with IBA in the range of 4.55 mg L-1 to 5.25 mg L-1. The best predicted response was obtained at a combination of 0.5 mg L-1 2,4-D + 3.53 mg L-1 IBA, from which the predicted values of the model were 100.0 of %SEC and 1.4323 g of ΔCW. The experimental values were very close to those predicted by RSM, which suggests that a computer-based experimental design it is very useful to optimize protocols for successful embryogenic callus induction in sugarcane.

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“…Furthermore, it could act as a putative inhibitor of SE because of its lower expression levels in embryogenic callus than in non-embryogenic callus and somatic embryos [13]. In contrast, the expression of the BBM orthologous gene promotes the development of somatic embryos in other species such as Saccharum officinarum, C. canephora, and C. arabica [11,87,88]. Therefore, although BBM genes of F. × ananassa and other Rosaceae species were similar to its orthologous gene in other species, such as the model plant A. thaliana, the molecular function of this SE-related TF could depend on the species or even a particular plant lineage.…”

Section: Genes and Proteins Of Lafl-b Networkmentioning

confidence: 99%

Genome-Wide Analysis of Somatic Embryogenesis-Related Transcription Factors in Cultivated Strawberry (Fragaria × ananassa) and Evolutionary Relationships among Rosaceae Species

2021

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Somatic embryogenesis is a plant regeneration method commonly used in tissue culture. Its molecular mechanisms are well-known in model plants such as Arabidopsis thaliana L. LEAFY COTYLEDON1 (LEC1), LEAFY COTYLEDON2 (LEC2), FUSCA3 (FUS3), ABSCISIC ACID INSENSITIVE3 (ABI3), and BABYBOOM (BBM) genes are considered master regulators in the induction, growth, and maturation of somatic embryos. However, the study of these transcription factors in fruit crops with high agronomic and economic value such as cultivated strawberry (Fragaria × ananassa Duch.) and other Rosaceae species is scarce. The purpose of this study was the in silico characterization of LEC1, ABI3, FUS3, LEC2, and BBM(LAFL-B) genes from F. × ananassa genome and the study of the evolutionary relationships within the Rosaceae family. Synteny analyses and molecular evolutionary rates were performed to analyze the evolution of each transcription factor within the Rosaceae family. Synteny was conserved between F. × ananassa and other Rosaceae genomes, and paralogous genes were selected through negative selection. Additionally, the exon–intron organization and multiple alignments showed that gene structure and DNA-binding domains were conserved in F. ×ananassa transcription factors. Finally, phylogenetic trees showed close evolutionary relationships between F. × ananassa and its orthologous proteins in the Rosoideae subfamily. Overall, this research revealed novel insights in the LAFL-B network in F. × ananassa and other species of the Rosaceae family. These results provide useful in silico information and new resources for the establishment of more efficient propagation systems or the study of ploidy effects on somatic embryogenesis.

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Expression of somatic embryogenesis-related genes in sugarcane (Saccharum officinarum L.)

Cited by 16 publications

References 53 publications

Indirect organogenesis for high frequency shoot regeneration of two cultivars of Sansevieria trifasciata Prain differing in fiber production

Indirect organogenesis for high frequency shoot regeneration of two cultivars of Sansevieria trifasciata Prain differing in fiber production

Use of Response Surface Methodology on Medium Components Optimization for Embryogenic Callus Induction in Sugarcane (Saccharum Sp.) Cv. Cp 72-2086

Genome-Wide Analysis of Somatic Embryogenesis-Related Transcription Factors in Cultivated Strawberry (Fragaria × ananassa) and Evolutionary Relationships among Rosaceae Species

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