Expression of Selenoprotein Genes Is Affected by Heat Stress in IPEC-J2 Cells

Cao, Lei; Tang, Jieyuan; Li, Qiang; Xu, Jingyang; Jia, Gang; Liu, Guangmang; Chen, Xiaoling; Shang, Haiying; Cai, Jingyi; Zhao, Hua

doi:10.1007/s12011-015-0604-0

Cited by 21 publications

(20 citation statements)

References 53 publications

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“…It was not surprising that HS increased gene and protein expression of HSP70 ( P < 0.01) in the differentiated C2C12 cells (Fig. 3 ), which was consistent with previous studies 30 , 41 .…”

Section: Discussionsupporting

confidence: 92%

“…However most of their functions are still unknown. Our previous study showed that both mRNA and protein expression of selenoprotein encoding genes were influenced by HS for 24 h in IPEC-J2 cells, and 4 selenoprotein genes ( GPX3 , DIO2 , SELENOK , SELENOS ) were up-regulated ( P < 0.05) and six selenoprotein genes ( GPX2 , GPX6 , TXNRD1 , SELENOH , SELENOM , MSRB1 ) were down-regulated ( P < 0.05 or as indicated) in IPEC-J2 cells by HS 30 . Interestingly, in this study, selenoprotein encoding genes (except DIO2 ) were globally up-regulated by HS in C2C12 cells, which suggesting their potential roles against HS-induced cell damage (Fig.…”

Section: Discussionmentioning

confidence: 81%

“…MDA is used as a biomarker to measure the level of oxidative stress in an organism 68 , and the decreased levels of MDA indicate cells were not in an oxidative stress condition. Our previous results shows that HS has limited effect on antioxidant measurements in porcine IPEC-J2 cells, and MDA exhibits a decreasing tendency in HS stressed cells 30 . It seems oxidative stress is not the major factor for C2C12 cells damages induced by HS, possibly the up-regulation of selenoprotein encoding genes contribute to preventing the increasing of MDA.…”

Section: Discussionmentioning

confidence: 88%

“…Two wells of cells were pooled together and in each treatment six samples were collected ( n = 6). The qPCRs procedure and relative mRNA abundance quantification were conducted as previously described using 2 −ΔΔCt method 30 , 69 . For each measurement, all samples were run on the same plate.…”

Section: Methodsmentioning

confidence: 99%

“…Selenoproteins have been involved in the regulation of redox balance, protection protein from oxidized damage, immunomodulatory, cell apoptosis, protein folding, and degradation of misfolded proteins in endoplasmatic reticulum (ER) 29 . Our previous studies showed that selenoprotein encoding genes were influenced by HS in a porcine small intestinal epithelial cell line (IPEC-J2) 30 , which suggested that they play important roles in cells under HS. However, the metabolic impact of HS on skeletal muscle and expression of selenoprotein encoding genes remain unclear, and it is necessary to explore the impact of HS on expression of selenoproteins using skeletal muscle cells model.…”

Section: Introductionmentioning

confidence: 99%

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Damage to the myogenic differentiation of C2C12 cells by heat stress is associated with up-regulation of several selenoproteins

Tang

Yan

et al. 2018

Sci Rep

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This study was conducted to profile the selenoprotein encoding genes or proteins in mouse C2C12 cells and integrate their roles in the skeletal cell damage induced by heat stress (HS). Cells were cultured at 37.0 °C or 41.5 °C for 4, 6 or 8 days. The mRNA expression of 24 selenoprotein encoding genes and abundance of 5 selenoproteins were investigated. HS suppressed myogenic differentiation and impaired the development of muscle myotubes. HS down-regulated (P < 0.01) mRNA abundance of MYOD and MYOGENIN, and decreased (P < 0.01) MYOGENIN protein expression, HS elevated (P < 0.01) HSP70 and (P < 0.01) the ratio of BCL-2 to BAX at both mRNA and protein level. Meanwhile, HS up-regulated (P < 0.01–0.05) expressions of 18, 11 and 8 selenoprotein encoding genes after 4, 6 and 8 days of hyperthermia, and only down-regulated (P < 0.01) DIO2 after 6 and 8 days of hyperthermia, respectively. Furthermore, HS influenced expression of selenoproteins and up-regulated (P < 0.01–0.05) GPX1, GPX4 and SEPN1 after 6 days of HS. The damage to development of mouse skeletal muscle myotubes by HS accompanied with the up-regulation of both selenoprotein encoding genes and proteins, which suggested a potential protective effect of selenoprotein on hyperthermia associated damage in C2C12 cells.

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“…It was not surprising that HS increased gene and protein expression of HSP70 ( P < 0.01) in the differentiated C2C12 cells (Fig. 3 ), which was consistent with previous studies 30 , 41 .…”

Section: Discussionsupporting

confidence: 92%

Section: Discussionmentioning

confidence: 81%

Section: Discussionmentioning

confidence: 88%

Section: Methodsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 3 more Smart Citations

Damage to the myogenic differentiation of C2C12 cells by heat stress is associated with up-regulation of several selenoproteins

Tang

Yan

et al. 2018

Sci Rep

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mTORC1 signaling activation increases intestinal stem cell activity and promotes epithelial cell proliferation

Zhou

Huang

Qin

et al. 2019

Journal Cellular Physiology

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The crypt‐villus axis of the intestine undergoes a continuous renewal process that is driven by intestinal stem cells (ISCs). However, the homeostasis is disturbed under constant exposure to high ambient temperatures, and the precise mechanism is unclear. We found that both EdU+ and Ki67+ cell ratios were significantly reduced after exposure to 41°C, as well as the protein synthesis rate of IPEC‐J2 cells, and the expression of ubiquitin and heat shock protein 60, 70, and 90 were significantly increased. Additionally, heat exposure decreased enteroid expansion and budding efficiency, as well as induced apoptosis after 48 hr; however, no significant difference was observed in the apoptosis ratio after 24 hr. In the process of heat exposure, the mechanistic target of rapamycin complex 1 (mTORC1) signaling pathway was significantly inhibited in both IPEC‐J2 cells and enteroids. Correspondingly, treatment of IPEC‐J2 and enteroids with the mTORC1 agonist MHY1485 at 41°C significantly attenuated the inhibition of proliferation and protein synthesis, increased the ISC activity, and promoted expansion and budding of enteroid. In summary, we conclude that the mTORC1 signaling pathway regulates intestinal epithelial cell and stem cell activity during heat exposure‐induced injury.

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Selenium exerts protective effects against heat stress‐induced barrier disruption and inflammation response in jejunum of growing pigs

Liu

Tang

et al. 2021

J Sci Food Agric

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BACKGROUND Heat stress (HS) has a negative impact on the intestinal barrier and immune function of pigs. Selenium (Se) may improve intestinal health through affecting selenoproteins. Thus we investigate the protective effect of new organic Se (2‐hydroxy‐4‐methylselenobutanoic acid, HMSeBA) on jejunal damage in growing pigs upon HS and integrate potential roles of corresponding selenoproteins. RESULTS HS decreased the villus height and increased (P < 0.05) the protein abundance of HSP70, and downregulated (P < 0.05) protein levels of tight junction‐related proteins (CLDN‐1 and OCLD). HS‐induced jejunal damage was associated with the upregulation of four inflammation‐related genes and ten selenoprotein‐encoding genes, downregulation (P < 0.05) of four selenoprotein‐encoding genes and decreased (P < 0.05) the protein abundance of GPX4 and SELENOS. Compared with the HS group, HMSeBA supplementation not only elevated the villus height and the ratio of V/C (P < 0:05), but also reduced (P < 0.05) the protein abundance of HSP70 and MDA content, and increased (P < 0.05) the protein abundance of OCLD. HMSeBA supplementation downregulated the expression of seven inflammation‐related genes, changed the expression of 12 selenoprotein‐encoding genes in jejunum mucosa affected by HS, and increased the protein abundance of GPX4, TXNRD1 and SELENOS. CONCLUSION Organic Se supplementation beyond nutritional requirement alleviates the negative effect of HS on the jejunum of growing pigs, and its protective effect is related to the response of corresponding selenoproteins. © 2021 Society of Chemical Industry.

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Expression of Selenoprotein Genes Is Affected by Heat Stress in IPEC-J2 Cells

Cited by 21 publications

References 53 publications

Damage to the myogenic differentiation of C2C12 cells by heat stress is associated with up-regulation of several selenoproteins

Damage to the myogenic differentiation of C2C12 cells by heat stress is associated with up-regulation of several selenoproteins

mTORC1 signaling activation increases intestinal stem cell activity and promotes epithelial cell proliferation

Selenium exerts protective effects against heat stress‐induced barrier disruption and inflammation response in jejunum of growing pigs

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