Expression of Salivary S100A7 Levels in Stage I Oral Submucous Fibrosis: A Clinical and Laboratory Study

Raffat, Muhammad Arsalan; Hadi, Naila Irum; Alghamdi, Osama; Al‐Aali, Khulud Abdulrahman; Deeb, Modhi Al; Abduljabbar, Tariq; Vohra, Fahim

doi:10.31557/apjcp.2020.21.4.1115

Cited by 12 publications

(8 citation statements)

References 21 publications

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“…We found 92 DEGs in NFs and 698 DEGs in CAL27 cells. Some of the DEGs have previously been reported associated with OSF and/or OSCC, like S100A7 [ 33 – 36 ], RPS28 [ 37 ] and CYP26B1 [ 38 , 39 ]. Increased expression of CYP26B1 was identified in betel quid (BQ)-related OSCC tumor tissues, and could be a novel biomarker for BQ-related oral and pharyngeal cancers [ 38 , 39 ].…”

Section: Discussionmentioning

confidence: 99%

Low-dose arecoline regulates distinct core signaling pathways in oral submucous fibrosis and oral squamous cell carcinoma

et al. 2023

BMC Oral Health

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Background Betel nut chewing plays a role in the pathogenesis of oral submucous fibrosis (OSF) and oral squamous cell carcinoma (OSCC). As the major active ingredient of the betel nut, the effect of arecoline and its underlying mechanism to OSF and OSCC pathogenesis remain unclear. Methods Next-generation sequencing-based transcriptome and dRRBS analysis were performed on OSF and OSCC cells under low-dose arecoline exposure. Functional analyses were performed to compare the different roles of arecoline during OSF and OSCC pathogenesis, and key genes were identified. Results In this study, we identified that low-dose arecoline promoted cell proliferation of both NFs and OSCC cells via the acceleration of cell cycle progression, while high-dose arecoline was cytotoxic to both NFs and OSCC cells. We performed for the first time the transcriptome and methylome landscapes of NFs and OSCC cells under low-dose arecoline exposure. We found distinct transcriptome and methylome profiles mediated by low-dose arecoline in OSF and OSCC cells, as well as specific genes and signaling pathways associated with metabolic disorders induced by low-dose arecoline exposure. Additionally, low-dose arecoline displayed different functions at different stages, participating in the modulation of the extracellular matrix via Wnt signaling in NFs and epigenetic regulation in OSCC cells. After exposure to low-dose arecoline, the node roles of FMOD in NFs and histone gene clusters in OSCC cells were found. Meanwhile, some key methylated genes induced by arecoline were also identified, like PTPRM and FOXD3 in NFs, SALL3 and IRF8 in OSCC cells, indicating early molecular events mediated by arecoline during OSF and OSCC pathogenesis. Conclusions This study elucidated the contribution of low-dose arecoline to OSF and OSCC pathogenesis and identified key molecular events that could be targeted for further functional studies and their potential as biomarkers.

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Section: Discussionmentioning

confidence: 99%

Low-dose arecoline regulates distinct core signaling pathways in oral submucous fibrosis and oral squamous cell carcinoma

et al. 2023

BMC Oral Health

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“…We found that S100A7 was remarkably upregulated in ESCC and performed well as an independent prognostic factor. In addition, numerous studies have reported that S100A7 can not only function within the cell but can also be secreted and detected in serum (lung squamous cell carcinoma), 34 saliva (oral squamous cell carcinoma), 35 urine (melanoma), 36 and other body fluids. Moreover, S100A7 was upregulated in the serum of patients with lung squamous cell carcinoma 34 .…”

Section: Discussionmentioning

confidence: 99%

S100A7 as a potential diagnostic and prognostic biomarker of esophageal squamous cell carcinoma promotes M2 macrophage infiltration and angiogenesis

Zheng

Liu

et al. 2021

Clinical & Translational Med

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“…CD34 is a cluster of differentiation molecule that is seen on several cells inside the body. It is a glycoprotein that is present on the surface of the cell, which works as a cell-cell adhesion factor [ 8 , 9 , 10 ]. Cells that express CD34 are endothelial cells of blood vessels excluding lymphatics (except pleural lymphatics), mast cells, a sub-population of dendritic cells (which are factor XIIIa negative) in the interstitium and around the adnexa of the dermis of the skin.…”

Section: Introductionmentioning

confidence: 99%

Expression of CD34 and α-SMA Markers in Oral Squamous Cell Carcinoma Differentiation. A Histological and Histo-Chemical Study

Maqsood

Ali

Zaffar

et al. 2020

IJERPH

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To reduce morbidity and mortality rates of OSCC cases, early diagnosis, assessment of behavior and prognostic estimates are vital. This study analyzed the expression of CD34 and alpha smooth muscle actin (α-SMA) in OSCC, to establish their significance in diagnosis and prognosis. Primary cases of OSCC, diagnosed with excisional biopsy at multiple cancer treatment centers, were included. Tissue sections were embedded and stained with H & E for histological differentiation and invasion of tumor vessel. Immunohistochemistry was performed using antibodies against CD34 and α-SMA. The chi-square and Pearson correlation coefficient (r) tests were applied for data analysis. Eighty patients with fifty males (62.5%) and thirty females (37.5%) and mean age of 45 ± 14.1 years were evaluated. Buccal mucosa was the most common site for OSCC lesions [36 (45%)]; 47.5% of lesions were moderately differentiated and 33.8% were well-differentiated lesions. Invasion of tumor vessels was observed in 35% of specimens. A significant association was seen between CD34 expression and histological grading of OSCC (p < 0.002). Among all poorly differentiated OSCC specimens, expression of CD 34 was low and α-SMA was high. CD 34 is a critical prognostic factor in OSCC diagnosis and increased α-SMA-positive myofibroblasts may indicate aggressive OSCC behavior.

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Expression of Salivary S100A7 Levels in Stage I Oral Submucous Fibrosis: A Clinical and Laboratory Study

Abstract: Pakistan is one of the countries in SouthEast Asia that have a broad habit of areca nut and smokeless chewable tobacco because their products are readily accessible that leads to increased OSF incidence in this country

Cited by 12 publications

References 21 publications

Low-dose arecoline regulates distinct core signaling pathways in oral submucous fibrosis and oral squamous cell carcinoma

Low-dose arecoline regulates distinct core signaling pathways in oral submucous fibrosis and oral squamous cell carcinoma

S100A7 as a potential diagnostic and prognostic biomarker of esophageal squamous cell carcinoma promotes M2 macrophage infiltration and angiogenesis

Expression of CD34 and α-SMA Markers in Oral Squamous Cell Carcinoma Differentiation. A Histological and Histo-Chemical Study

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