Expression of RNA polymerase IV and V in Oryza sativa

Abstract

RNA polymerase IV and V are principal players in the RdDM pathway, where their current study has shown interaction of several factors that control DNA silencing of intergenic regions and siRNA production. DNA silencing is an important process during cell differentiation, nuclear structure and viral control. However, RNA pol IV and V are yet to be study in model monocot systems like Oryza sativa that can provide further information on genetic silence mechanism in plats. We show the expression pattern of these polymerases in tissues extracts of Oryza sativa. Detectable amounts of these polymerases are found in specific adult plant tissues and particularly expressed during somatic embryogenesis but not during early stages of normal embryo development. The use of synthetic auxin leads to an induction of both RNA pol IV and V in scutellum tissue where nuclear localization may be required for genome reorganization and gene silencing.

Several Isoforms for Each Subunit Shared by RNA Polymerases are Differentially Expressed in the Cultivated Olive Tree (Olea europaea L.)

“…A specific transcriptional response in early development has been described for the genes involved in DNA methylation, which were upregulated during the 6-month follow-up (Jiménez-Ruiz et al, 2018). Interestingly, the role of RNA pols IV and V in RNA-directed DNA methylation has been clearly demonstrated (Huang et al, 2009;Ream et al, 2009;Haag and Pikaard, 2011;Haag et al, 2014;Zhou and Law, 2015) by acting during development (Pikaard and Tucker, 2009;Moo et al, 2012;Haag et al, 2014). However, the NRPE5 gene for RNA pol V (and/or IV) did not specifically and differently modulate its expression.…”

Alteraciones anatómicas e hiperplasia inducidas por Euphorbia mosaic virus aislamiento Yucatan Peninsula en el mesófilo

“…A los 15 días después de la inoculación (ddi) se recolectaron las hojas número nueve y 10, denominadas "hojas con infección sistémica" porque emergieron después de la inoculación. Estas se fijaron y se embebieron en parafina, se hicieron cortes transversales de 8-10 μm en el centro de las hojas tal como se reportó previamente (Moo et al, 2012). Se adquirieron imágenes con el objetivo 40x de un microscopio confocal láser modelo Olympus FV100 SW y se procesaron con el software FV10 ASW versión 4.1.…”

“…Se midió el grosor de la hoja y se contó el número de células por mm 2 de cuatro plantas con síntomas moderados o severos a los 15 ddi. Se graficó el número de células y made, as reported previously (Moo et al, 2012). Images were taken using a 40x lens of an Olympus FV100 SW laser confocal microscope, and they were processed using the FV10 ASW software version 4.1.…”

RNA polymerases IV and V are involved in olive fruit development

“…Transcription in bacteria and archaea is carried out by a single multimeric RNA polymerase, while most eukaryotes contains three multimeric complexes (RNA pol I, II, and III) [1][2][3]. Furthermore, plants contain two additional RNA pols (IV and V) that have evolved from RNA pol II [4][5][6][7][8][9][10][11][12]. RNA pol I consists of 14 subunits and synthesizes precursor rRNA 45S (35S in yeast) of the three largest rRNAs [3,[13][14][15].…”

“…Plant-specific RNA pol IV and V, which have evolved from RNA pol II through duplication and functional divergence, also contain 12 subunits. These two enzymes, that are involved in epigenetic regulation synthesize siRNAs, which play roles in transcriptional silencing via RNA-directed DNA methylation (RdDM), and non-coding RNAs participating in plant growth, development, response to environmental changes or plant immunity [4][5][6][7][8][9][10][11][12][22][23][24].…”