Expression of Renal Vitamin D and Phosphatonin-Related Genes in a Sheep Model of Osteoporosis

Dittmer, Keren E.; Chernyavtseva, Anastasia; Marshall, Jonathan; Cabrera, Diana; Wolber, Frances M.; Kruger, Marlena C.

doi:10.3390/ani12010067

Cited by 4 publications

(1 citation statement)

References 44 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Vitamin D regulates the homeostasis of calcium. Since the vitamin D receptor (VDR) mediates the benefits of vitamin D, down regulating VDR results in calcium shortage [23]; [24] [25]. Calmodulin is crucial for the Ca signaling system, controlling gene expression, metabolism, and facilitating calcium absorption through calcium-triggered Calcium/ calmodulin dependent protein kinase (CaMKK), mediated by CAMK1 and CAMK4 [26].…”

Section: Introductionmentioning

confidence: 99%

Gene Expression of Oxidative/Antioxidative Markers, VDR, CAMK4 and Ceruloplasmin in Baladi Sheep a with Minerals Deficiency

Emam,

Ghanem,

Abdel-Raof

et al. 2025

Egyptian Journal of Veterinary Sciences

View full text Add to dashboard Cite

HE OBJECTIVE of this work was to elaborate gene expression and serum profile of oxidative/antioxidant and bone markers associated with minerals deficiency and their correlation with mineral deficiency in sheep. The study was carried out on 26 selected diseases cases out of survey on 250 sheep suffered from mineral deficiency compared to 10 apparent healthy control sheep based on clinical examination. Biochemically, there was a significant (P<0.05) decline in Ca, P, Mg, Cu, Zn, Se, Fe, TAC, GPx, SOD, CAT, OC and CP, whereas MDA level was significantly increased (P<0.05). Gene expression of SOD, CAT, GPX1, VDR, CAMK4 and ceruloplasmin genes were significantly down-regulated (P<0.05) in diseased sheep. A contrary tendency was evoked by the gene OXSR1.The variability in studied genes alongside alterations in the serum profile of investigated markers could be a reference guide for limiting the mineral deficiency in sheep through prescribing an efficient nutritional management strategy for sheep flocks that includes the essential macro-and micro aspects, and not depend just on grazing as a primary source of feed to sustain sheep health and output.

show abstract

Section: Introductionmentioning

confidence: 99%

Gene Expression of Oxidative/Antioxidative Markers, VDR, CAMK4 and Ceruloplasmin in Baladi Sheep a with Minerals Deficiency

Emam,

Ghanem,

Abdel-Raof

et al. 2025

Egyptian Journal of Veterinary Sciences

View full text Add to dashboard Cite

show abstract

Klotho reduces the risk of osteoporosis in postmenopausal women: a cross-sectional study of the National Health and Nutrition Examination Survey (NHANES)

et al. 2023

View full text Add to dashboard Cite

Background Osteoporosis (OP) is one of the diseases that endanger the health of the elderly population. Klotho protein is a hormone with anti-aging effects. A few studies have discussed the relationship between Klotho and OP. However, there is still a lack of research on larger populations. This study aims to evaluate the association between OP and Klotho in American postmenopausal women. Methods This is a retrospective study. We searched the National Health and Nutrition Examination Survey (NHANES) database and collected data of 3 survey cycles, finally involving 871 postmenopausal women over 50 years old in the present study. All participants took dual-energy X-ray absorptiometry examination and serum Klotho testing at the time of investigation. After adjusting the possible confounding variables, a multivariate regression model was employed to estimate the relationship between OP and Klotho proteins. Besides, the P for trend and restricted cubic spline (RCS) were applied to examine the threshold effect and calculate the inflection point. Results Factors influencing the occurrence of OP included age, ethnicity, body mass index and Klotho levels. Multivariate regression analysis indicated that the serum Klotho concentration was lower in OP patients than that in participants without OP (OR[log2Klotho] = 0.568, P = 0.027). The C-index of the prediction model built was 0.765, indicating good prediction performance. After adjusting the above-mentioned four variables, P values for trend showed significant differences between groups. RCSs revealed that when the Klotho concentration reached 824.09 pg/ml, the risk of OP decreased drastically. Conclusion Based on the analysis of the data collected from the NHANES database, we propose a correlation between Klotho and postmenopausal OP. A higher serum Klotho level is related to a lower incidence of OP. The findings of the present study can provide guidance for research on diagnosis and risk assessment of OP.

show abstract

Sheep as a large animal model for hearing research: comparison to common laboratory animals and humans

Lue,

Oliver,

Neeff

et al. 2023

Lab Anim Res

View full text Add to dashboard Cite

Sensorineural hearing loss (SNHL), caused by pathology in the cochlea, is the most common type of hearing loss in humans. It is generally irreversible with very few effective pharmacological treatments available to prevent the degenerative changes or minimise the impact. Part of this has been attributed to difficulty of translating “proof-of-concept” for novel treatments established in small animal models to human therapies. There is an increasing interest in the use of sheep as a large animal model. In this article, we review the small and large animal models used in pre-clinical hearing research such as mice, rats, chinchilla, guinea pig, rabbit, cat, monkey, dog, pig, and sheep to humans, and compare the physiology, inner ear anatomy, and some of their use as model systems for SNHL, including cochlear implantation surgeries. Sheep have similar cochlear anatomy, auditory threshold, neonatal auditory system development, adult and infant body size, and number of birth as humans. Based on these comparisons, we suggest that sheep are well-suited as a potential translational animal model that bridges the gap between rodent model research to the clinical use in humans. This is especially in areas looking at changes across the life-course or in specific areas of experimental investigation such as cochlear implantation and other surgical procedures, biomedical device development and age-related sensorineural hearing loss research. Combined use of small animals for research that require higher throughput and genetic modification and large animals for medical translation could greatly accelerate the overall translation of basic research in the field of auditory neuroscience from bench to clinic.

show abstract

Expression of Renal Vitamin D and Phosphatonin-Related Genes in a Sheep Model of Osteoporosis

Cited by 4 publications

References 44 publications

Gene Expression of Oxidative/Antioxidative Markers, VDR, CAMK4 and Ceruloplasmin in Baladi Sheep a with Minerals Deficiency

Gene Expression of Oxidative/Antioxidative Markers, VDR, CAMK4 and Ceruloplasmin in Baladi Sheep a with Minerals Deficiency

Klotho reduces the risk of osteoporosis in postmenopausal women: a cross-sectional study of the National Health and Nutrition Examination Survey (NHANES)

Sheep as a large animal model for hearing research: comparison to common laboratory animals and humans

Contact Info

Product

Resources

About