Expression of Recombinant Stonustoxin Alpha Subunit and Preparation of polyclonal antiserum for Stonustoxin Neutralization Studies
Amir Sajjad Hojjati-Razgi,
Shahram Nazarian,
Hossein Samiei-Abianeh
et al.
Abstract:Stonustoxin (SNTX) is a lethal protein found in stone sh venom, responsible for many of the symptoms associated with stone sh envenomation. To counter stone sh venom challenges, antivenom is a wellestablished and effective solution. In this study, we aimed to produce the recombinant alpha subunit protein of stonustoxin from Synanceia horrida and prepare antibodies against it. The SNTXα gene sequence was sourced from GenBank and codon-optimized to match the codon usage of E. coli BL21 (DE3). This optimized sequ… Show more
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