Expression of Rapeseed Microsomal Lysophosphatidic Acid Acyltransferase Isozymes Enhances Seed Oil Content in Arabidopsis

Maisonneuve, Sylvie; Bessoule, Jean‐Jacques; Lessire, Reneݩ; Delseny, Michel; Roscoe, Thomas

doi:10.1104/pp.109.148247

Cited by 119 publications

(87 citation statements)

References 52 publications

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“…The membrane-bound glycerol 3-phosphate acyltransferase, AtGPAT1, has a necessary role in pollen development but has no significant effect on the oil content of Arabidopsis seeds (28). The overexpression of microsomal lysophosphatidic acid acyltransferase caused an enhanced TAG accumulation in seeds of B. napus (29). A soluble MGAT that has a role in TAG accumulation was identified from the immature peanut seeds (20).…”

Section: Discussionmentioning

confidence: 98%

Oleosin Is Bifunctional Enzyme That Has Both Monoacylglycerol Acyltransferase and Phospholipase Activities

Parthibane

Rajakumari

Venkateshwari

et al. 2012

Journal of Biological Chemistry

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Background: Seeds with high oil content have more oleosins than those with low oil content. However, the exact role of oleosins in oil accumulation is unclear. Results: We demonstrate that oleosin 3 is involved in diacylglycerol biosynthesis and phosphatidylcholine hydrolysis. Conclusion: Oleosin, a structural protein, is involved in biosynthesis and mobilization of plant oils. Significance: This study provides direct evidence for the presence of an alternate route for the biosynthesis of triacylglycerol from monoacylglycerol.

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Section: Discussionmentioning

confidence: 98%

Oleosin Is Bifunctional Enzyme That Has Both Monoacylglycerol Acyltransferase and Phospholipase Activities

Parthibane

Rajakumari

Venkateshwari

et al. 2012

Journal of Biological Chemistry

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“…Recently, overexpression of a Brassica napus LPAT isoform with homology to A. thaliana LPAT2 resulted in enhanced TAG accumulation in Arabidopsis seeds (40). Genetic studies with Arabidopsis LPAT2 have been limited because female gametophyte development is disrupted in the loss-of-function mutants (41); its direct role in TAG biosynthesis in Arabidopsis should perhaps be revisited.…”

Section: Conventional Kennedy Pathwaymentioning

confidence: 99%

Compartmentation of Triacylglycerol Accumulation in Plants

Chapman

Ohlrogge

2012

Journal of Biological Chemistry

386

352

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Triacylglycerols from plants, familiar to most people as vegetable oils, supply 25% of dietary calories to the developed world and are increasingly a source for renewable biomaterials and fuels. Demand for vegetable oils will double by 2030, which can be met only by increased oil production. Triacylglycerol synthesis is accomplished through the coordinate action of multiple pathways in multiple subcellular compartments. Recent information has revealed an underappreciated complexity in pathways for synthesis and accumulation of this important energyrich class of molecules. Regulation of Fatty Acid Supply by PlastidsPlant fatty acid (FA) 2 synthesis differs from almost all other eukaryotes in two fundamental features. First, unlike the cytosolic location in other kingdoms, FAs for triacylglycerol (TAG) and membrane synthesis are produced in the plastid compartment of plant cells: chloroplasts in green tissues and proplastids (or leucoplasts) in non-green tissues. Second, the plant FA synthase (FAS) is a dissociable complex with separate proteins for the acyl carrier protein (ACP) and each enzyme (1). After assembly of C 16 and C 18 acyl chains by FAS and desaturation of C 18:0 to C 18:1 , FAs destined for TAG assembly are released from ACP in the plastid stroma by chain-terminating acyl-ACP thioesterases. Two classes of thioesterases designated FATA and FATB are responsible for hydrolysis of unsaturated and saturated acyl-ACPs, respectively, and thus determine in large part the chain length and saturated FA content of plant oils (2). The FA products of FATA and FATB are activated to CoA before export to the endoplasmic reticulum (ER). The subsequent reactions of TAG synthesis belong to the so-called "eukaryotic" pathway of glycerolipid synthesis, which occurs outside the plastid (3, 4). An overview of the compartmentalization of FA supply for TAG is shown in Fig. 1. FA production by plastids can limit TAG accumulation in seeds (5, 6), so increasing flux through FA biosynthesis may perhaps have the single greatest influence on the amount of TAG produced in plant tissues. As in bacteria, fungi, and animals, both in vitro and in vivo evidence indicates that acetylCoA carboxylase (ACCase) is a key rate-determining step that controls FA biosynthesis. ACCase activity is under complex regulation by light, phosphorylation, thioredoxin, PII protein, and product feedback control (7,8). Of course, the flux of carbon to FA synthesis can have multiple regulatory steps, which may explain why efforts to increase seed oil by up-regulating ACCase were only modestly successful (9).Transcriptional regulation of the production of FA for TAG biosynthesis is most directly controlled by the WRINKLED1 transcription factor (7, 10, 11). Arabidopsis wri1 mutants are reduced by 80% in seed oil, and overexpression of WRI1 can increase oil content in seeds of several plants. Targets of WRI1 include ACCase, many enzymes of FAS, and key enzymes and transporters that provide pyruvate and acetyl-CoA in the plastid. Thus, WRI1 can be considered as ...

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“…These seeds were dried in open tubes in desiccators for 24 h before weighing and counting (Maisonneuve et al 2010). For lipid analysis of transgenic tobacco, four aliquots of 50 tobacco seeds from each line were homogenised three times (15 000 rpm, 30 s each) in 800 mL hexane/isopropanol (3 : 2, v/v, 0.01% BHT) mixture with a superfine homogenizer (Fluko Equipment Shanghai Co., Ltd, Shanghai, China).…”

Section: Seed Weight Determination and Lipid Analysismentioning

confidence: 99%

“…For lipid analysis of transgenic tobacco, four aliquots of 50 tobacco seeds from each line were homogenised three times (15 000 rpm, 30 s each) in 800 mL hexane/isopropanol (3 : 2, v/v, 0.01% BHT) mixture with a superfine homogenizer (Fluko Equipment Shanghai Co., Ltd, Shanghai, China). After dispersion, the homogenate was subjected to direct transmethylation as described by Maisonneuve et al (2010). This method involves adding 2 mL of methanol containing 2.5% H 2 SO 4 (v/v) to each sample and heating at 90 C for 90 min.…”

Section: Seed Weight Determination and Lipid Analysismentioning

confidence: 99%

Characterisation of DGAT1 and DGAT2 from Jatropha curcas and their functions in storage lipid biosynthesis

Yang

Wang

et al. 2014

Functional Plant Biol.

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Abstract. Diacylglycerol acyltransferases (DGATs) catalyse the final step of triacylglycerol (TAG) biosynthesis of the Kennedy pathway, and play a critical role during TAG accumulation in developing oleaginous seeds. In this study, the molecular cloning and characterisation of two DGAT genes, JcDGAT1 and JcDGAT2, from jatropha (Jatropha curcas L., a potential biodiesel plant) is presented. Using heterogonous overexpression techniques, both JcDGAT1 and JcDGAT2 were able to restore TAG biosynthesis in a yeast mutant H1246 strain, and enhance the quantity of TAG biosynthesis by 16.6 and 14.3%, respectively, in strain INVSc1. In transgenic tobacco, overexpression of JcDGAT1 and JcDGAT2 resulted in an increase in seed oil content of, respectively, 32.8 and 31.8%. Further, the functional divergence of JcDGAT1 and JcDGAT2 in TAG biosynthesis was demonstrated by comparing the fatty acid compositions in both the transgenic yeast and tobacco systems. In particular, JcDGAT2 incorporated a 2.5-fold higher linoleic acid content into TAG than JcDGAT1 in transgenic yeast and exhibited a significant linoleic acid substrate preference in both yeast and tobacco. This study provides new insights in understanding the molecular mechanisms of DGAT genes underlying the biosynthesis of linoleic acids and TAG in plants.

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Expression of Rapeseed Microsomal Lysophosphatidic Acid Acyltransferase Isozymes Enhances Seed Oil Content in Arabidopsis

Abstract: Addendum to: Maisonneuve S, Bessoule J-J, Lessire R, Delseny M, Roscoe TJ. Expression of rapeseed microsomal lysophosphatidic acid acyltransferase isozymes enhances seed oil content in Arabidopsis.

Cited by 119 publications

References 52 publications

Oleosin Is Bifunctional Enzyme That Has Both Monoacylglycerol Acyltransferase and Phospholipase Activities

Oleosin Is Bifunctional Enzyme That Has Both Monoacylglycerol Acyltransferase and Phospholipase Activities

Compartmentation of Triacylglycerol Accumulation in Plants

Characterisation of DGAT1 and DGAT2 from Jatropha curcas and their functions in storage lipid biosynthesis

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