Expression of RANKL/OPG during bone remodeling in vivo

Tanaka, Hiroshi; Mine, Takatomo; Ogasa, Hiroyoshi; Taguchi, Toshihiko; Liang, Chunyu

doi:10.1016/j.bbrc.2011.07.001

Cited by 50 publications

(39 citation statements)

References 21 publications

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“…Bone marrow ablation induces the rapid activation of bone synthetic activity. Initially, bone formation markers (e.g., Alk-P) were expressed in the bone formation phase, followed by the stimulation of OPG expression in the bone resorption phase (Tanaka et al 2011). This finding is in line with our observation that, following PTX, there was a 4-to 8-week lag between changes in Alk-P and the subsequent parallel changes in OPG levels.…”

Section: Discussionmentioning

confidence: 99%

Association between Increased Serum Osteoprotegerin Levels and Improvement in Bone Mineral Density after Parathyroidectomy in Hemodialysis Patients

Zheng

Chu

et al. 2012

Tohoku J. Exp. Med.

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Secondary hyperparathyroidism (SHPT) is a common complication in chronic renal disease. Osteoprotegerin (OPG), an extracellular cytokine receptor secreted by osteoblasts, can promote bone formation by inhibiting the function of osteoclasts. Hemodialysis (HD) patients have elevated serum OPG levels. OPG secretion can be suppressed with high parathyroid hormone (PTH) levels. HD patients with refractory SHPT can benefit from parathyroidectomy (PTX) treatment, but the changes of serum OPG, bone turnover markers and bone mineral density (BMD) following PTX in HD patients remain unclear. In this study, patients on maintenance HD who received PTX for refractory SHPT (n = 28) were prospectively followed for 1 year. Serum intact PTH (iPTH), alkaline phosphatase (Alk-P), and OPG were measured serially; BMD was measured pre-PTX and at 1 year after PTX. After PTX, serum iPTH levels reduced profoundly. Serum Alk-P levels increased rapidly, peaking at 2 weeks post-PTX, while serum OPG levels gradually increased at 2 weeks after PTX and peaked at 2 months. BMD improved in both femoral neck (FN; cancellous and cortical bone) and lumbar spine (LS; cancellous bone). Higher baseline iPTH levels were associated with greater FN and LS BMD improvements at one year after PTX. The increment of serum OPG was correlated with the increase in LS BMD, implying that inhibition of osteoclastic bone resorption may improve BMD within the first year after PTX. These findings suggest that PTX removes the suppressive effects of high PTH on OPG secretion, resulting in the increased serum OPG levels that may contribute to BMD improvement.

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Section: Discussionmentioning

confidence: 99%

Association between Increased Serum Osteoprotegerin Levels and Improvement in Bone Mineral Density after Parathyroidectomy in Hemodialysis Patients

Zheng

Chu

et al. 2012

Tohoku J. Exp. Med.

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“…RANKL can bind to RANK on the surface of pre-osteoclasts, leading to activation of osteoclasts (11). OPG, secreted by many cell types including osteoblasts, is a decoy receptor for RANKL that interrupts the interaction between RANKL and RANK, thereby inhibiting bone resorption (12). Therefore, the OPG/RANK/RANKL signaling pathway regulates the development and activation of osteoclasts, and consequently affects bone resorption.…”

Section: Originalmentioning

confidence: 99%

The effects of marsupialization on bone regeneration adjacent to keratocystic odontogenic tumors, and the mechanisms involved

Zhao

Man

et al. 2017

Journal of Oral Science

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Bone resorption in the jaws is one of the most severe complications of keratocystic odontogenic tumors (KCOTs), and can be treated by either enucleation or marsupialization. However, the effects of marsupialization on bone regeneration adjacent to KCOTs, and the mechanisms involved, are still unclear. In this study, samples of 27 KCOTs were collected (20 before marsupialization and 7 after marsupialization) to detect the expression of bone regeneration-related molecules adjacent to KCOTs by immunohistochemistry and real-time quantitative polymerase chain reaction (qPCR). The results showed that bone formation was significantly enhanced in the KCOT capsule wall adjacent to bone after marsupialization, as demonstrated by alkaline phosphatase activity assay and immunostaining for bone morphogenetic protein. Moreover, immunohistochemistry revealed that osteoprotegerin (OPG) was up-regulated in the KCOT capsule wall adjacent to bone after marsupialization, while receptor activator of nuclear factor-κB ligand (RANKL) was down-regulated. Real-time qPCR also demonstrated increased expression of OPG after marsupialization, accompanied by a decrease in the expression of osteoclastogenesis-related molecules such as cathepsin K, matrix metalloproteinase-9, NFATc1, RANK and RANKL. This study provides further evidence that marsupialization may promote bone regeneration adjacent to KCOTs partially through regulation of the OPG/RANK/RANKL signaling pathway.

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“…Numerous hormones and cytokines are involved in regulating osteoblasts and osteoclasts, among which the RANKL/RANK/OPG system plays a key role. Osteoblasts and bone marrow stromal cells secrete RANKL, which combines with RANK on osteoclasts/broken bone precursor cells to mediate osteoclast maturation and increase bone resorption (Tanaka et al, 2011). Simultaneously, they express OPG, which competitively combines with RANKL to inhibit osteoclast generation and maturation ( Marie and Halbout, 2008).…”

Section: Introductionmentioning

confidence: 99%

Opg/Rankl mRNA dynamic expression in the bone tissue of ovariectomized rats with osteoporosis

Li¹,

Liang²,

Shi³

et al. 2015

Genet. Mol. Res.

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ABSTRACT.We established animal models of osteoporosis in ovariectomized rats to detect osteoprogerin (Opg)/receptor activator of nuclear factor-kB ligand (Rankl) mRNA expression levels in the tibias and serum estradiol concentrations at different time points. Sixty Sprague-Dawley female rats were randomly selected and divided into an ovariectomized (OVX) group and sham-operated (SHAM) group. In the SHAM group, only a small amount of abdominal fat and tissues was removed from the rats. Ten rats in each group were sacrificed at 0, 6, and 12 months after establishing the animal models (12 weeks). Opg mRNA expression and serum estradiol concentration in the OVX group were significantly lower than those in the SHAM group (P < 0.05). In contrast, Rankl mRNA expression in the OVX group was significantly higher than that in the SHAM group (P < 0.05). In the OVX group, Opg mRNA expression and serum estradiol concentrations decreased C. Li et al. 9216©FUNPEC-RP www.funpecrp.com.br Genetics and Molecular Research 14 (3): 9215-9224 (2015) significantly from 0 to 12 months (P < 0.05), whereas Rankl mRNA expression increased significantly (P < 0.05). Opg mRNA expression and serum estradiol concentrations in the OVX group continually decreased, whereas Rankl mRNA expression continually increased. The Opg/Rankl ratio showed a decrease. The OPG/RANKL ratio may be a key factor affecting the osteoblast-mediated reaction.

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Expression of RANKL/OPG during bone remodeling in vivo

Cited by 50 publications

References 21 publications

Association between Increased Serum Osteoprotegerin Levels and Improvement in Bone Mineral Density after Parathyroidectomy in Hemodialysis Patients

Association between Increased Serum Osteoprotegerin Levels and Improvement in Bone Mineral Density after Parathyroidectomy in Hemodialysis Patients

The effects of marsupialization on bone regeneration adjacent to keratocystic odontogenic tumors, and the mechanisms involved

Opg/Rankl mRNA dynamic expression in the bone tissue of ovariectomized rats with osteoporosis

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