Expression of rabbit defensin NP-1 gene in transgenic tobacco plants and its activity against bacterial wilt

R, Fu; Peng, Yufa; Cao, Guangcheng; Ma, Ju; Chen, Caixia; Zhang, Liming; Li, Wenbin; Sun, Yuhan

doi:10.1007/bf02883446

Cited by 11 publications

(3 citation statements)

References 16 publications

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“…Plants that had been transformed with NaD1 gene containing the CTPP do not exhibit this abnormal phenotype, suggesting the CTPP either protects the plant from a toxic part of the molecule or it targets the protein to the vacuole where it is sequestered. 36 It has been demonstrated that plants expressing human, 37 rabbit 38 or insect 39 defensin acquire resistance against fungal pathogens, indicating a functional homology next to the already known structural homology, between defensins originating from different eukaryotic kingdoms. Aert et al 37 showed that that the antifungal plant defensin RsAFP2 from radish induces apoptosis and concomitantly triggers activation of caspases or caspase-like proteases in the human pathogen Candida albicans.…”

Section: Plant Defensins Play Roles In Protection Of Seeds and Varioumentioning

confidence: 99%

Plant defensins

Stotz¹,

Thomson

Wang

2009

Plant Signaling & Behavior

197

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Section: Plant Defensins Play Roles In Protection Of Seeds and Varioumentioning

confidence: 99%

Plant defensins

Stotz¹,

Thomson

Wang

2009

Plant Signaling & Behavior

197

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“…The NP-1 disease-resistant mechanism was combined with a virus coat protein to lose their biological activity (Chen et al, 2001b). However, only three strains showed better inhibitory effects in six positive transgenic plants, which may be owing to the following: first, the low expression of the NP-1 gene; there is a threshold level of transgene-derived transcripts, and disease-resistant mechanisms cannot be triggered until the threshold level in transgenic plants is reached (Fu et al, 1998); second, the genetic variation of other disease-resistant related genes during the process of transformation; and third, false positives.…”

Section: Discussionmentioning

confidence: 99%

Transfer and expression of the rabbit defensin NP-1 gene in lettuce (Lactuca sativa)

Song

Xiong

et al. 2017

Genet. Mol. Res.

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ABSTRACT. Lettuce (Lactuca sativa L.) is an annual plant of the daisy family, Asteraceae, with high food and medicinal value. However, the crop is susceptible to several viruses that are transmitted by aphids and is highly vulnerable to post-harvest diseases, as well as insect and mammal pests and fungal and bacterial diseases. Here, the rabbit defensin gene NP-1 was transferred into lettuce by Agrobacteriummediated transformation to obtain a broad-spectrum disease-resistant lettuce. Transgenic lettuce plants were selected and regenerated on selective media. The presence of the NP-1 gene in these plants was confirmed by western blot analyses. Resistance tests revealed native defensin NP-1 expression conferred partial resistance to Bacillus subtilis and Pseudomonas aeruginosa, which suggests new possibilities for lettuce disease resistance.

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“…Thus, gene constructions including sequences coding for AMPs have been expressed on model or crop plants providing different degrees of protection against plant pathogens. Transgenic plants introduced with defensin Fu et al 1998 Note: CaMV = Cauliflower Mosaic Virus, E12Ω = the 5' enhancer sequence from CaMV35S promoter + omega sequence from TMV, E7ΩIn = synthesized high expression vector, D35S = double CaMV35S + omega sequence from TMV, FMV = Figwort Mosaic Virus, PiII = promoter from proteineaseII inhibitor gene, OSMp = osmotin promoter, ND = Not Determined, TSP=total soluble protein, * the transgenic plant displayed increased resistance to the tested organisms unless otherwise specified. or 10 8 CFU/mL of P. carotovorum subsp.…”

Section: Transgenic Plants Expressing Antimicrobial Peptidesmentioning

confidence: 99%

Application of Antimicrobial Peptides for Disease Control in Plants

Jung¹,

Kang²

2014

Plant Breed. Biotech.

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This is an Open-Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/3.0) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited. ABSTRACT A large number of antimicrobial peptides (AMPs) from different organisms have been characterized to date. AMPs are small molecular weight proteins with broad spectrum antimicrobial activity against bacteria, viruses, and fungi. Several diseases in plants could result in serious losses and decreasing quality and safety of agricultural products. Being an agent for plant defense, AMPs kill target cells through diverse mechanisms once in a target microbial membrane. Cathelicidins, defensins and thionins are the three major groups of epidermal AMPs in human and plants. Plant AMPs are structurally and functionally diverse and can be directed against other organisms, like herbivorous insects. The biological activity of plant AMPs primarily depends on interactions with membrane lipids. Several antimicrobial peptides have been expressed in transgenic plants to confer disease protection. Antimicrobial peptides are interesting compounds that can be efficiently exploited for disease control in plants in a way that complies with the strict regulations on the efficacy and safety of disease control strategy.

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Expression of rabbit defensin NP-1 gene in transgenic tobacco plants and its activity against bacterial wilt

Cited by 11 publications

References 16 publications

Plant defensins

Plant defensins

Transfer and expression of the rabbit defensin NP-1 gene in lettuce (Lactuca sativa)

Application of Antimicrobial Peptides for Disease Control in Plants

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