Expression of prostate stem cell antigen (PSCA) in prostate cancer: A tissue microarray study of Iranian patients

ALDH1 in Combination with CD44 as Putative Cancer Stem Cell Markers are Correlated with Poor Prognosis in Urothelial Carcinoma of the Urinary Bladder

“…Immunohistochemical staining was performed on TMA slides (Superfrost plus, Thermo Scientific, Germany) prepared from formalin-fixed paraffin-embedded tissues (FFPE) using a protocol as previously described (Mohsenzadegan et al, 2013;Taeb et al, 2014). Briefly, after deparaffinizationin xylene, tissues were rehydrated by immersion in decreasing grades of ethanol and endogenous peroxidase activity was blocked by immersing the tissues for 20 min in methanol containing 0.3% hydrogen peroxide and retrieved by boiling in a microwave for 20 minutes with sodium citrate buffer (pH 6.0).…”

Prostate cancer relevant antigens and enzymes for targeted drug delivery

“…55, 56 Moreover, the PSCA expression level in high-grade prostatic intraepithelial neoplasm is fourfold higher than that in benign prostatic hyperplasia. 575858 An in situ analysis of 126 prostate cancer specimens, including high-grade prostatic intraepithelial neoplasia and androgen-dependent and androgen-independent tumors, showed moderate to strong PSCA expression in more than 85% of the samples. 56 The higher level of PSCA expression is directly associated with the advanced stages, higher degree and androgen independency of the disease.…”

“…59 Its expression level increases in cases of higher Gleason score, advanced tumor stage and progression of disease to androgen-independent state. 57, 59 Moreover, the limited expression in normal prostate tissues, direct connection with tumor grade or stage, and expression on the surface of tumor cells suggest that PSCA could become a prominent target in drug delivery to prostate cancer. 58 …”

Expression of EMSY, a Novel BRCA2-link Protein, is Associated with Lymph Node Metastasis and Increased Tumor Size in Breast Carcinomas

“…Immunohistochemical detection of EMSY was performed on TMA slides (Superfrost plus, Thermo Scientific, Germany) using a standard chain polymerconjugated (Envision) technique as described previously (Madjd et al, 2011;Madjd et al, 2012;Taeb et al, 2014) applying specific Rabbit polyclonal antibody (ab 4580, abcam, Cambridge, UK). After deparaffinization in xylene and rehydration through graded alcohol, slides were immersed in methanol/hydrogen peroxide for 10 min to block endogenous peroxidase activity.…”