Expression of proliferating cell nuclear antigen in the endometrium of mares during estrus and at early diestrus

Uterine acute post-breeding inflammation is a physiological tissue response to the entry of exogenous elements, with persistent endometritis being the main pathology responsible for subfertility in the mare (Equus ferus caballus; Linnaeus, 1758). Mares can be classified as susceptible or resistant to endometritis according to their ability to remove intrauterine fluid within 48 hr after experimental inoculation. Endometrial biopsy is a technique that is commonly used to establish the degree of lesions that can affect the fertility of the mare. Endometrial histomorphometry is an objective and highly precise diagnostic method. The aim of this study was to compare, during oestrus, the endometrial histomorphometry of mares previously classified as susceptible (SM) or resistant (RM) to endometritis. Endometrial biopsies from 24 mares at the oestrus phase of the cycle were obtained. For the histomorphometric analysis, samples were histologically processed and subjected to routine Haematoxylin-Eosin staining. For the evaluation, the variables were considered as follows: 1-Height of the lining and glandular epithelia (Lining SM = 15.9 μm vs. RM = 13.3 μm; Glandular SM = 15.0 μm vs. RM = 13.0 μm); 2-Perpendicular diameters of endometrial glands (SM = 51.3 μm vs. RM = 44.8 μm); 3-Number of endometrial glands per field (SM = 24.8 glands/field vs. RM = 20.5 glands/field). The results from this study suggest the existence of a relationship between the studied characteristics and the susceptibility/resistance to post-breeding endometritis in mares. Thus, increased epithelial height, greater glandular density and greater development of the glands during oestrus would be related to a higher susceptibility to endometritis.

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Modern methods of research of reproductive organs of mares in pedigree horse breeding

Dubrovskaya¹

2022

Head of Animal Breeding

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The most important aspect of herd reproduction in horse breeding is the quality of the breeding nucleus of the breeding stock. Currently, the system of domestic comprehensive reproductive evaluation includes five main methods such as rectal, ultrasound, vaginal, cytological and bacteriological studies of the genital tract of mares, which precede visual examination of the external genitalia and the collection of information about the reproductive history of the mare. The purpose of the work is to give an overview and description of the methods of comprehensive evaluation of the reproductive status of mares, which are used in modern pedigree horse breeding. The methods of reproductive evaluation of mares, gives the main advantages of each method and their predictive value when used comprehensively to increase the level of reproduction in the industry have been described in the article. It has been carried out that a complete reproductive evaluation of mares is necessary in rare and complex cases. However, the use of methods of rectal, vaginoscopic, ultrasound, cytological and bacteriological diagnostics of the genital tract of mares is quite within the power of veterinarians and breeding specialists and they are available under production conditions. Their application requires skills in the technical side of the issue and the established system for the prompt dispatch of biomaterial to the nearest, but reliable veterinary or medical bacteriological laboratory. It is well known that even in small horse breeding farms of the Russian Federation, there are currently means and opportunities for the qualitative evaluation of the reproductive system of mares.

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Expression of proliferating cell nuclear antigen in the endometrium of mares during estrus and at early diestrus

Cited by 3 publications

References 16 publications

Effect of Mycobacterium cell wall fraction on endometrial histomorphometry of mares resistant and susceptible to persistent breeding-induced endometritis

Effect of Mycobacterium cell wall fraction on endometrial histomorphometry of mares resistant and susceptible to persistent breeding-induced endometritis

Comparative histomorphological study of endometrium in mares

Modern methods of research of reproductive organs of mares in pedigree horse breeding

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