Expression of prolactin-related protein I at the fetomaternal interface during the implantation period in cows

Yamada, Osamu; Todoroki, Junichi; Kizaki, Keiichiro; Takahashi, Toshio; Imai, Kyoichi; Patel, OV; Schuler, LA; Hashizume, Kazuyoshi

doi:10.1530/rep.0.1240427

Cited by 48 publications

(67 citation statements)

References 1 publication

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…Increased stimulation of the uterus would, in theory, provide a more favourable environment for the growth of the conceptus and, as a consequence, an increase in the numbers of binucleate cells available to secrete pregnancy-enhancing proteins such as CSH1 (Wooding 1992) and PAG9 (Green et al 2000). In the present study, trophoblast tissue of the large conceptuses appeared to be at a very early developmental stage, with binucleate cells just beginning to differentiate, as there were more transcripts of DKK1 and PRP1 than CSH1 present in the trophoblast tissue (Yamada et al 2002;Ledgard et al 2009a). The large conceptuses from SR cows tended to have greater mean total numbers of transcripts for all the trophoblastrelated genes examined, indicating that it was possible that SR conceptuses had progressed slightly more in development than those from IR cows.…”

Section: Evaluation Of the Bovine Uterine Environmentmentioning

confidence: 48%

Evaluation of the uterine environment early in pregnancy establishment to characterise cows with a potentially superior ability to support conceptus survival

Ledgard

Meier

Peterson

2011

Reprod. Fertil. Dev.

View full text Add to dashboard Cite

Abstract. During previous investigations, the capacity of the cow to secrete prostaglandin in response to oxytocin has been linked with pregnancy outcome. The objective of the present study was to evaluate the predictive value of prostaglandin release to identify groups of cows as potentially superior (SR, low prostaglandin release) or inferior (IR, high prostaglandin release) for pregnancy outcome and to utilise these cows to investigate factors that contribute to optimum uterine conditions for early pregnancy. Animals were synchronised and received an in vitro-derived blastocyst on Day 7 post-oestrus. Tissues (trophoblast and endometrial) and uterine luminal fluid (ULF) were recovered 10 days later. Pregnancy rates were 94 and 78% for SR and IR cows, respectively. Of the pregnant SR cows, 69% had larged conceptuses (.24 cm) in contrast to 43% IR of cows. IR cows with small conceptuses (,12 cm) had significantly lower mean Day 3 and 5 post-oestrous progesterone concentrations than cows with large conceptuses. The expression of factors involved in the prostaglandin pathway, pregnancy and conceptus development were analysed via quantitative RT-PCR and Western blot analysis. Investigation of 16 endometrial gene transcripts indicated no differences between IR and SR cows except for osteopontin expression which, in uteri with large conceptuses, was 2-fold greater in SR than IR cows (P ¼ 0.02). There was greater expression of CTGF, OXTR, PGES, PGHS2 and UTMP mRNA in uteri of SR and IR cows that had large compared with small conceptuses (P , 0.05). More IFNT protein was recovered in SR compared with IR ULF (P , 0.03). SR cows with large conceptuses had less TIMP2 and legumain protein in their gravid, compared with their non-gravid horns (P # 0.02) whereas IR cows did not. The predictive value of prostaglandin release in response to oxytocin challenge does not appear to be an effective indicator of subsequent pregnancy rates in cows. Differences between the two groups appear to be associated with subtle differences in progesterone and uterine protein concentrations that may be related to differences in conceptus size.

show abstract

Section: Evaluation Of the Bovine Uterine Environmentmentioning

confidence: 48%

Evaluation of the uterine environment early in pregnancy establishment to characterise cows with a potentially superior ability to support conceptus survival

Ledgard

Meier

Peterson

2011

Reprod. Fertil. Dev.

View full text Add to dashboard Cite

show abstract

“…Around the implantation period, we observed two distinct phenomena: ECM breakdown and PRP gene expression in the CAR [33,35]. Morphological evidence was observed only in a limited area of the endometrium.…”

Section: Role Of Mmps In Endometrial Remodeling Around the Time Of Immentioning

confidence: 84%

“…PLs, PRPs, and uterine milk protein have been identified as other genes whose expression increased along with the progress of gestation; these changes were observed around day 60. Some of these genes were localized in trophoblastic binucleate cells (BNCs) [30][31][32][33][34]. These CAR-and/or ICAR-specific expression genes may play functional roles in implantation and early placentation.…”

Section: Gene Expression Profiles In the Extraembryonic Membrane And mentioning

confidence: 99%

Analysis of Uteroplacental-specific Molecules and Their Functions During Implantation and Placentation in the Bovine

Hashizume

2007

Journal of Reproduction and Development

View full text Add to dashboard Cite

Abstract. In cattle, the mechanisms underlying implantation and placental development are still unclear. Synepitheliochorial placentation in cattle is noninvasive, and thus generates limited interest in terms of degradation and remodeling of endometrial tissues. The overall purpose of this study was three-fold: (1) to examine the gene circuitry around the implantation window, (2) to understand development of the placenta during the peri-implantation period by using a uteroplacental cDNA microarray, and (3) to study the roles of molecules involved in endometrial remodeling. Bovine trophoblastic binucleate cell-specific molecules, such as pregnancy-associated glycoproteins (PAGs), placental lactogen (PL), and prolactin-related proteins (PRPs), were markedly expressed in binucleate cells (BNCs) around implantation. The expression of PRP-1 was specific to the caruncular (CAR) area of the gravid uterine horn. Gelatinases (MMP-2 and -9) in association with heparanase may be central to endometrial remodeling. In situ hybridization analyses of PAGs, PRPs, PL, and heparanase suggested that BNCs expressed these molecules simultaneously. Future studies will further investigate the specific roles of these molecules in placentogenesis. The uteroplacental cDNA microarray presented cascades of molecular signatures not only for the endometrium but also for the intricate dialogue at the level of the feto-maternal interface in cattle. Placentome morphogenesis potentially parallels the dynamic multigenic circuitry and regulates the cell cycle in the endometrium. The roles of BNCs and their secreted molecules remain an enigma, particularly with regard to the adhesion process and endometrial remodeling, which is the focus of this study.

show abstract

“…MNC production of IFNT is well known as a factor that signals the recognition of pregnancy; BNCs may play a major role in the establishment of the fetal-maternal interface, namely, BNC fuses with an epithelial cell in endometrium and starts to raise placental villi at around day 20 of gestation (Wooding 1992, Yamada et al 2002. Although cell lines are a convenient tool to analyze and understand the functions and the mechanisms of trophoblastic cell lineages, few bovine trophoblastic cell lines have been available (Talbot et al 2000, Shimada et al 2001.…”

Section: Discussionmentioning

confidence: 99%

Bone morphogenetic protein 4 accelerates the establishment of bovine trophoblastic cell lines

Suzuki¹,

Koshi²,

Imai³

et al. 2011

Reproduction

View full text Add to dashboard Cite

Trophoblastic cells play a crucial role in implantation and placentogenesis. A large proportion of the failures of conception in cows occur in the peri-implantation period, which are known as early embryo losses. In exploring this critical phenomenon, trophoblastic cell lines can provide substantial information. Unfortunately, there are few cell lines for this purpose in cattle because of the difficulty of raising successive cell stock in the long term. In this study, 12 new cell lines were established using bone morphogenetic protein 4 (BMP4). BMP4 stimulated embryonic cells to enter the trophoblastic cell lineage but there were no significant differences between intact and BMP4-treated groups. Only one out of 49 embryos developed trophoblastic cells in the intact group. Finally, 12 cell lines were maintained for around 30 passages, and they retained trophoblastic characteristics and expressed bovine trophoblastic genes: placental lactogen, interferon-t, pregnancy-associated glycoprotein 1, and prolactin-related protein 1. Although the gene expression patterns were different among cell lines and depended on the cells, there was no significant relationship between the expression intensities of genes and the treatment dose of BMP4. All of them expressed bovine POU domain class 5 transcription factor 1 and caudal-type homeobox 2. The expression of these genes was confirmed by quantitative RT-PCR and immunohistochemical detection. These results suggest that BMP4 is involved in the raising of trophoblast cell lines from early embryonic cells and the newly developed cell lines can provide different types of bovine trophoblastic cells with different cell lineages. This may constitute a significant new tool for the examination of trophoblastic differentiation.

show abstract

Expression of prolactin-related protein I at the fetomaternal interface during the implantation period in cows

Cited by 48 publications

References 1 publication

Evaluation of the uterine environment early in pregnancy establishment to characterise cows with a potentially superior ability to support conceptus survival

Evaluation of the uterine environment early in pregnancy establishment to characterise cows with a potentially superior ability to support conceptus survival

Analysis of Uteroplacental-specific Molecules and Their Functions During Implantation and Placentation in the Bovine

Bone morphogenetic protein 4 accelerates the establishment of bovine trophoblastic cell lines

Contact Info

Product

Resources

About