Expression of Prolactin Gene in Human Decidua During Pregnancy Studied by In Situ Hybridization Histochemistry.

Tadokoro, N; Koibuchi, Noriyuki; Ohtake, Hideki; Kawatsu, Takeshi; Kato, Y.; Yamaoka, Sadao; Kumasaka, T

doi:10.1507/endocrj.42.537

Cited by 7 publications

(3 citation statements)

References 26 publications

(40 reference statements)

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“…As far as we know, this hypothesis remains to be explored, but there is indirect evidence to support it. Studies using in situ hybridization of placental tissue have shown that the PRL mRNA signal is restricted to stromal cells both in early and term decidua [21][22][23].…”

Section: Discussionmentioning

confidence: 99%

Regional Localization of Immunoreactive c-<i>fos</i> and Prolactin in Human Endometrium during the Normal Menstrual Cycle

Reis¹,

Ribeiro²,

Spritzer³

1999

Gynecol Obstet Invest

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The objective of the present study was to evaluate the cyclic changes and regional localization of immunoreactive c-fos and prolactin (PRL) in the human endometrium, using immunohistochemistry. Immunoreactive PRL was found in the epithelium of 9.1% of the proliferative specimens and in 55.6% of the secretory specimens (p < 0.05, Fisher’s exact test). In the endometrial stroma, immunoreactive PRL was present in 9.1 and 66.7% of the proliferative and secretory samples, respectively (p < 0.01). Immunoreactive c-fos predominated in the stroma and was identified in 54.5% of the specimens in the proliferative phase, but in only 7.1% of those in the secretory phase (p < 0.05). The progesterone/estradiol ratio was lower in the patients expressing immunoreactive c-fos (median = 13.1 ng/ml) compared to those who did not (median = 84.5 ng/ml, p < 0.05). We conclude that immunoreactive c-fos is found mostly in stromal cells during the proliferative phase of the menstrual cycle, and is sharply reduced during the secretory phase, when the endometrium is under progesterone stimulation – attested by PRL production.

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Section: Discussionmentioning

confidence: 99%

Regional Localization of Immunoreactive c-<i>fos</i> and Prolactin in Human Endometrium during the Normal Menstrual Cycle

Reis¹,

Ribeiro²,

Spritzer³

1999

Gynecol Obstet Invest

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“…The decidua basalis contributes to the formation of the placental base plate, and this rigid part of the decidua directs the interstitial invasion of trophoblasts to form the anchoring columnar villi. In contrast, the capsular decidua is laxer and, because of its location, represents the major source of PRL in the amniotic fluid before it fuses with the parietal decidua (53).…”

Section: Decidual Prl and Its Autocrine Functionsmentioning

confidence: 97%

Immunomodulatory role of decidual prolactin on the human fetal membranes and placenta

et al. 2023

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The close interaction between fetal and maternal cells during pregnancy requires multiple immune-endocrine mechanisms to provide the fetus with a tolerogenic environment and protection against any infectious challenge. The fetal membranes and placenta create a hyperprolactinemic milieu in which prolactin (PRL) synthesized by the maternal decidua is transported through the amnion-chorion and accumulated into the amniotic cavity, where the fetus is bedded in high concentrations during pregnancy. PRL is a pleiotropic immune-neuroendocrine hormone with multiple immunomodulatory functions mainly related to reproduction. However, the biological role of PRL at the maternal-fetal interface has yet to be fully elucidated. In this review, we have summarized the current information on the multiple effects of PRL, focusing on its immunological effects and biological significance for the immune privilege of the maternal-fetal interface.

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“…1983; Taii et al. 1984; DiMattia et al 1990; Brown & Bethea 1994; Tadokoro et al. 1995; Harigaya et al.…”

Section: Introductionmentioning

confidence: 99%

Prolactin gene expression in mouse pancreatic islets

Harigaya

Komori

Watanabe

et al. 2002

Animal Science Journal

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Mouse prolactin (mPRL) is a single‐chain polypeptide hormone, that is generally secreted from the prolactin cells of the anterior pituitary gland into the blood. However, recent studies have reported the ectopic gene expression of prolactin in several tissues, including the mammary gland, mammary tumors, lymphocytes, brain and decidua. In the present study, PRL gene expression was determined by reverse transcription polymerase chain reaction (RT‐PCR) followed by Southern blot analysis and the nucleotide sequence analysis of the amplified complementary DNA (cDNA) . In situ hybridization was also performed to determine the localization of mPRL mRNA in the pancreas of adult male and female mice during pregnancy and lactation. Total ribonucleic acid (RNA) extracted from the pancreas was reverse transcribed, followed by polymerase chain reaction (PCR) with primers specific for a part of the mPRL cDNA. An amplified product was detected in all samples from the pancreas. This product hybridized specifically to a probe overlapping the entire mPRL cDNA sequence in Southern blot analysis. Nucleotide sequence analysis indicated that the amplified product was completely identical to the pituitary PRL cDNA sequence. Moreover, PRL mRNA was observed in the pancreatic islets by in situ hybridization. These results suggest that PRL mRNA is expressed in mouse pancreatic islets and that PRL may have some autocrine and/or paracrine roles in these tissues.

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Expression of Prolactin Gene in Human Decidua During Pregnancy Studied by In Situ Hybridization Histochemistry.

Abstract: Abstract.The prolactin (PRL) gene is known to be expressed not only in the anterior pituitary but also in the decidualized human endometrium.

Cited by 7 publications

References 26 publications

Regional Localization of Immunoreactive c-<i>fos</i> and Prolactin in Human Endometrium during the Normal Menstrual Cycle

Regional Localization of Immunoreactive c-<i>fos</i> and Prolactin in Human Endometrium during the Normal Menstrual Cycle

Immunomodulatory role of decidual prolactin on the human fetal membranes and placenta

Prolactin gene expression in mouse pancreatic islets

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