Expression of progesterone receptor isoforms in corpora lutea of human subjects: correlation with serum oestrogen and progesterone concentrations

Misao, Ryou; Nakanishi, Yoshihito; Iwagaki, Shigenori; Fujimoto, Jiro; Tamaya, Teruhiko

doi:10.1093/molehr/4.11.1045

Cited by 33 publications

(22 citation statements)

References 38 publications

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“…In the non-human primate, PR A levels decreased throughout the luteal phase while PR B remained unchanged . However, in the human corpus luteum, both PR A and PR B isoforms were reduced in the late-luteal phase (Misao et al 1998). Certainly the pattern of PR B expression in luteinised granulosa cells is similar to that of PR A/B in our experiments.…”

Section: Discussionsupporting

confidence: 78%

“…In the primate corpus luteum, Western blotting suggested that the major PR variant is the B isoform . In human corpora lutea, however, PR A/B mRNA was found in higher concentrations than PR B mRNA (Misao et al 1998, Ottander et al 2000 and PR A was confirmed to be higher using Western blotting (Misao et al 1998). It seems clear, however, that both PR isoforms are expressed in the corpus luteum.…”

Section: Discussionmentioning

confidence: 88%

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The effect of human chorionic gonadotrophin on the expression of progesterone receptors in human luteal cells in vivo and in vitro

Duncan¹,

Gay²,

Maybin³

2005

Reproduction

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The human corpus luteum expresses genomic progesterone receptors (PRs) suggesting that progesterone may have an autocrine or paracrine role in luteal function. We hypothesised that the reduction in luteal PR reported in the late-luteal phase augmented progesterone withdrawal and had a role in luteolysis. We therefore tested the hypothesis that luteal rescue with human chorionic gonadotrophin (hCG) would maintain PR expression. PR was immunolocalised to different cell types in human corpora lutea (n 5 35) from different stages of the luteal phase and after luteal rescue with exogenous hCG. There was no change in the staining intensity of theca-lutein cell or stromal cell PR throughout the luteal phase or after luteal rescue. In the late-luteal phase, granulosa-lutein cell PR immunostaining was reduced (P < 0.05) but the trend to reduction was also seen after luteal rescue with hCG (P 5 0.055). To further investigate the effect of hCG on granulosa-lutein cell PR expression, an in vitro model system of cultured human luteinised granulosa cells was studied. Cells were cultured for 12 -13 days exposed to different patterns of hCG and aminoglutethamide to manipulate progesterone secretion (P < 0.0001). Expression of PR A/B and PR B isoforms was examined by quantitative real-time RT-PCR. PR A/B mRNA was lower (P < 0.05) after 11 -13 days of culture than after 7 days of culture. This reduction could not be prevented by hCG in the presence (P < 0.05) or absence (P < 0.05) of stimulated progesterone secretion. The expression of PR B mRNA showed a similar pattern (P 5 0.054). Simulated early pregnancy in vivo and hCG treatment of luteinised granulosa cells in vitro did not appear to prevent the down-regulation of PR seen during luteolysis.

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Section: Discussionsupporting

confidence: 78%

Section: Discussionmentioning

confidence: 88%

The effect of human chorionic gonadotrophin on the expression of progesterone receptors in human luteal cells in vivo and in vitro

Duncan¹,

Gay²,

Maybin³

2005

Reproduction

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“…P 4 mediates its actions via nuclear and membrane receptors (PGR and PGRMC1, PGRMC2 respectively). As shown in the present study, the expression of PGR dropped along with the development of porcine CL, similar to bovine CL (Misao et al 1998). Thus, it is likely that a negative regulatory loop exists between the intraluteal concentration of P 4 and expression of PGR in CL of cyclic pigs, as demonstrated in bovine CL (Misao et al 1998).…”

Section: Luteolysissupporting

confidence: 82%

Steroid hormones, prostanoids, and angiogenic systems during rescue of the corpus luteum in pigs

Przygrodzka¹,

Kaczmarek²,

Kaczyński³

et al. 2016

Reproduction

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In order to characterize the transition of the corpora lutea (CL) from acquisition of luteolytic sensitivity to rescue of luteal function: i) the expression of 38 factors associated with steroids, prostanoids, and angiogenic systems and ii) concentrations of the main hormones responsible for maintenance of CL function in cyclic and pregnant pigs were examined. Additionally, the effect of prostaglandin (PG) E 2 and F 2a on luteal function during the estrous cycle and pregnancy was evaluated in vitro. Significantly up-regulated gene expression was revealed in CL collected on day 14 of the estrous cycle (CYP19A1, ESR2, PTGS2, HIF1A, and EDN1) and on days 12-14 of pregnancy (SCARB1, PGRMC1, STAR, HSD3B1, NR5A1, PTGFR, PTGER4, and VEGFA). Elevated concentrations of estradiol-17b and PGE 2 occurred in CL on days 12 and 14 of pregnancy respectively, while an increased intraluteal PGF 2a content was noted on day 14 of the estrous cycle. Both PGs increased the synthesis of progesterone by cultured luteal slices obtained on day 14 of pregnancy, in contrast to the action of PGF 2a on the corresponding day of the estrous cycle. PGE 2 stimulated cAMP production via PTGER2 and PTGER4, while PGF 2a elevated the content of CREB in cultured luteal slices from CL of pregnant pigs. In silico analysis showed that infiltration of lymphocytes and apoptosis of microvascular endothelium were activated in CL on day 12 of the estrous cycle vs pregnancy. Summarizing, an abundance of E 2 and PGE 2 during pregnancy regulates specific pathways responsible for steroidogenesis, the prostanoid signaling system and angiogenesis during rescue from luteolysis in porcine CL.

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“…In the primate corpus luteum, Western blotting suggested that PR-B is the major isoform (Duffy et al 1997). In contrast, in human corpora lutea PR-A was confirmed to be higher using Western blotting (Misao et al 1998). The impacts of these differences are not fully understood yet.…”

Section: Gpermentioning

confidence: 97%

Induction of G protein-coupled estrogen receptor (GPER) and nuclear steroid hormone receptors by gonadotropins in human granulosa cells

Pavlik

Wypior

Hecht

et al. 2011

Histochem Cell Biol

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Estradiol and progesterone mediate their actions by binding to classical nuclear receptors, estrogen receptor α (ERα) and estrogen receptor β (ERβ) and progesterone receptor A and B (PR-A and PR-B) and the non-classical G protein-coupled estrogen receptor (GPER). Several animal knock-out models have shown the importance of the receptors for growth of the oocyte and ovulation. The aim of our study was to identify GPER in human granulosa cells (GC) for the first time. Moreover, the effect of different doses of gonadotropins on estrogen and progesterone receptors in the human ovary should be investigated as follicle stimulating hormone (FSH) and luteinizing hormone (LH) are also responsible for numerous mechanisms in the ovary like induction of the steroid biosynthesis. Human GC were cultured in vitro and stimulated with different doses of recombinant human FSH or LH. Receptor expression was analyzed by immunocytochemistry and quantitative real-time RT-PCR. GPER could be identified for the first time in human GC. It could be shown that high concentrations of LH increase GPER protein expression. Furthermore FSH and LH increased ERβ, PR-A and PR-B significantly on protein level. These findings were verified for high doses of FSH and LH on mRNA level. ERα was not affected with FSH or LH. We assume that gonadotropins induce GPER, ERβ and PR in luteinized granulosa cells.

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Expression of progesterone receptor isoforms in corpora lutea of human subjects: correlation with serum oestrogen and progesterone concentrations

Cited by 33 publications

References 38 publications

The effect of human chorionic gonadotrophin on the expression of progesterone receptors in human luteal cells in vivo and in vitro

The effect of human chorionic gonadotrophin on the expression of progesterone receptors in human luteal cells in vivo and in vitro

Steroid hormones, prostanoids, and angiogenic systems during rescue of the corpus luteum in pigs

Induction of G protein-coupled estrogen receptor (GPER) and nuclear steroid hormone receptors by gonadotropins in human granulosa cells

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