Expression of proapoptotic receptors Fas/Fas-L on sperm cells and levels of their soluble forms in seminal fluid from patients with varicocele

Razeghinia, Mohammad Sadegh; Sahebazzamani, Maryam; Soleymani, Saeed; Ketabchi, Ali-Asghar; Moqaddasi-Amiri, Mohammad

doi:10.1016/j.jri.2021.103443

Cited by 2 publications

(4 citation statements)

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“…Apoptosis is a type of cell death that depends on genetically coded signals or activity within dying cells. It is concerned with the growth and development of organs and tissues, immunity, metabolism and the removal of abnormal cells [ 34 , 35 ]. Furthermore, we observed that more cellular debris at the bottom of the medium, and “drawing” of the cells morphologically under the microscope after MH treatment, and the number of AML12 cells was reduced in a time-dependent manner after MH treatment.…”

Section: Discussionmentioning

confidence: 99%

Cold Stress Induced Liver Injury of Mice through Activated NLRP3/Caspase-1/GSDMD Pyroptosis Signaling Pathway

et al. 2022

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The body needs to generate heat to ensure basic life activities when exposed to cold temperatures. The liver, as the largest glycogen storage organ in the body and main heat-producing organ at rest, may play a role in chronic cold exposure. Recent studies suggested that pyroptosis plays a crucial role in liver diseases. However, the role of pyroptosis in cold stress-induced liver injury is not clear. Hence, in this study, we attempted to investigate the effects of chronic cold exposure on liver function, apoptosis, oxidative stress and inflammation in mice by establishing a mouse model of chronic cold exposure, and to investigate whether pyroptosis pathways are involved in the process of chronic cold exposure. In vivo, our results show that inflammatory cell infiltration and other pathological changes in liver cells and the activity of liver enzyme evidently increased in the serum and liver of cold-exposed mice, suggesting cold stress may result in liver injury. Remarkably, increased expression of heat shock protein 70 (HSP70) and HSP90 proteins proved the cold stress model is successfully constructed. Then, elevated levels of apoptosis, inflammation, oxidative stress and pyroptosis related proteins and mRNAs, such as cysteinyl aspartate specific proteinase-3 (Caspase-3), inducible nitric oxide synthase (iNOS), nuclear factor erythroid2-related factor 2 (Nrf2) and gasdermins D (GSDMD), confirmed that cold exposure activated apoptosis, oxidative stress and pyroptosis, and released inflammation cytokines. Meanwhile, in vitro, we got similar results as in vivo. Further, adding an NLR family pyrin domain containing 3 (NLRP3) inhibitors found that suppression expression of NLRP3 results in the essential proteins of pyroptosis and antioxidant evidently reduced, and adding GSDMD inhibitor found that suppression expression of GSDMD accompanies with the level of Nrf2 and heme oxygenase-1 (HO-1) obviously reduced. In summary, these findings provide a new understanding of the underlying mechanisms of the cold stress response, which can inform the development of new strategies to combat the effects of hypothermia.

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Section: Discussionmentioning

confidence: 99%

Cold Stress Induced Liver Injury of Mice through Activated NLRP3/Caspase-1/GSDMD Pyroptosis Signaling Pathway

et al. 2022

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“…

Dear Editor, We read with great interest the article by Razeghinia et al (2022) on the expression of proapoptotic receptors Fas/Fas-L on sperm cells and levels of their soluble forms in seminal fluid from patients with varicocele. We want to congratulate the authors for this successful article and make some contributions.The authors state that sperm apoptosis is a useful indicator of male infertility in the case of varicocele, while being unable to detect Fas and Fas-L on sperm cells.

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mentioning

confidence: 99%

“…To prove this, we performed semen analysis through a multiparameter flow cytometry method that excluded leukocytes from the sperm population (Perticarari et al, 2007(Perticarari et al, , 2008. In their paper the authors show cytograms of Fas and Fas-L expressions in the sperm population gated in the R1 region, based on Forward Scatter vs. Side Scatter (Razeghinia et al, 2022). The correct gate on sperm, on the other hand, should be made to exclude leukocytes, identified by specific markers such as CD45, or, even better, it should combine a vital dye to distinguish the live sperm population from dead sperm and other cellular debris (Ricci et al, 2009).…”

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confidence: 99%