Expression of prestin and Gata-3,-2,-1 mRNA in the rat organ of Corti during the postnatal period and in culture

Gross, Johann; Stute, Kerstin; Moller, Renate; Fuchs, Julia; Amarjargal, Nyamaa; Pohl, Elena E.; Angerstein, Maximilian; Smorodchenko, Alina; Mazurek, Birgit

doi:10.1016/j.heares.2009.12.017

Cited by 8 publications

(12 citation statements)

References 61 publications

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“…Previously, we had shown a close correlation to exist between Gata-3 and prestin mRNA levels under control and TH stimulated conditions over all segments, within the segments, and under the influence of gemfibrozil, a substance which induces Gata-3 (Gross et al, 2010). The present data extend this observation.…”

Section: Correlation Between Transcription Factor Gata-3 and Prestin supporting

confidence: 88%

“…A possible explanation for the formation of the gradient is the differential recruitment of coactivators or corepressors. As previously discussed, the apical-basal gradient cannot be explained by postnatal changes of the morphology of outer hair cells along the tonotopic axis (Gross et al, 2010). The prestin gradient seems to be involved in the frequency-dependent coupling of the basilar membrane motion and the inner hair cell stereociliar shear as discussed by Santos-Sacchi (2008).…”

Section: Discussion Retinoic Acid Is a Critical Component Of Prestin mentioning

confidence: 90%

“…Thus, the organotypic culture seems to be a good experimental model to study the factors involved in postnatal prestin expression. Recently, we observed that GATA-3,-2 expression displays an apical-basal gradient similar to that of the prestin mRNA levels (Gross et al, 2010). Because of the parallel changes in both prestin and GATA-3 mRNA expression, we hypothesized that the GATA-3,-2 transcription factors may have a regulatory role to play in prestin expression.…”

Section: Introductionmentioning

confidence: 94%

“…Instead of Gapdh we used the Tata box binding protein (Tbp) as a reference gene (Roon-Mom et al, 2005). Analyses had shown that the Tbp mRNA expression did not change significantly in either culture or postnatal development (Gross et al, 2010). Tbp is a transcription factor that binds specifically to a DNA sequence called the TATA box.…”

Section: Rna Preparation and Rt-pcrmentioning

confidence: 99%

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Effects of retinoic acid and butyric acid on the expression of prestin and gata‐3 in organotypic cultures of the organ of corti of newborn rats

Gross

Stute

Fuchs

et al. 2011

Developmental Neurobiology

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Prestin is the motor protein of the outer hair cells of the organ of Corti and a key factor in ensuring a high level of sensitivity of mammalian hearing. The factors that influence prestin expression are still largely unknown. We studied the effects of the application of retinoic acid, a ligand of a nuclear receptor, and of butyric acid, an inhibitor of histone deacetylase activity, on the expression of mRNA of prestin and Gata-3 in the organotypic culture of the organ of Corti of newborn rats using RT-PCR. Application of retinoic acid at concentrations of 1-50 μM results in a dose-dependent expression decrease after two days in culture. Treatment with sodium butyrate (0.5-2 mM) elevated the expression of prestin and Gata-3. Statistically significant correlations between Gata-3 and prestin mRNA levels were observed under all conditions. The data indicate that retinoid nuclear transcription factors, GATA-3 and histone acetylation/deacetylation processes may have a regulatory role to play in prestin expression.

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Section: Correlation Between Transcription Factor Gata-3 and Prestin supporting

confidence: 88%

Section: Discussion Retinoic Acid Is a Critical Component Of Prestin mentioning

confidence: 90%

Section: Introductionmentioning

confidence: 94%

Section: Rna Preparation and Rt-pcrmentioning

confidence: 99%

See 2 more Smart Citations

Effects of retinoic acid and butyric acid on the expression of prestin and gata‐3 in organotypic cultures of the organ of corti of newborn rats

Gross

Stute

Fuchs

et al. 2011

Developmental Neurobiology

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“…Prestin expression and incorporation in the plasma membrane begin from postnatal day zero and increase progressively in a time course coinciding with that of electromotility (Belyantseva et al 2000). In a previous study, we had shown that there is a close correlation between the mRNA levels of Gata-3 and prestin both in vitro and in vivo, which points to a role Gata-3 has to play in regulating prestin expression (Gross et al 2010). GATA-3 is a member of the zinc finger family and is essential to inner ear development (Karis et al 2001).…”

Section: Introductionmentioning

confidence: 67%

Expression Analysis of Prestin and Selected Transcription Factors in Newborn Rats

et al. 2011

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Transcription factors (TFs) have a central role to play in regulating gene expression. To analyze the co-expression patterns of selected TFs with the motor protein prestin of the outer hair cells, we applied an real-time PCR approach combining several kinds of information: (i) expression changes during postnatal development, (ii) expression changes by exposure of organotypic cultures of the organ of Corti to factors which significantly affect prestin expression [thyroid hormone (T4), retinoic acid (RA), butyric acid (BA), increased KCl concentration] and (iii) changes along the apical-basal gradient. We found that the mRNA levels of the TF Brn-3c (Pou4f3), a member of the POU family, are significantly associated with the regulation of prestin during postnatal development and in cultures supplemented with T4 (0.5 μM), BA (0.5-2.0 mM), and high KCl (50 mM) concentration. The mRNA level of the constitutively active TF C/ebpb (CCAAT/enhancer binding protein beta) correlates positively with the prestin expression during postnatal development and in cultures exposed to T4 and RA (50-100 μM). The mRNA levels of the calcium-dependent TF CaRF correlates significantly with the prestin expression in cultures exposed to T4 and high KCl concentration. The observed coexpression patterns may suggest that the TFs Brn-3c, C/ebpb, and Carf contribute to regulating the expression of prestin under the investigated conditions.

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Role of mitochondrial uncoupling protein 4 in rat inner ear

Smorodchenko¹,

Rupprecht²,

Fuchs³

et al. 2011

Molecular and Cellular Neuroscience

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Expression of prestin and Gata-3,-2,-1 mRNA in the rat organ of Corti during the postnatal period and in culture

Cited by 8 publications

References 61 publications

Effects of retinoic acid and butyric acid on the expression of prestin and gata‐3 in organotypic cultures of the organ of corti of newborn rats

Effects of retinoic acid and butyric acid on the expression of prestin and gata‐3 in organotypic cultures of the organ of corti of newborn rats

Expression Analysis of Prestin and Selected Transcription Factors in Newborn Rats

Role of mitochondrial uncoupling protein 4 in rat inner ear

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