Expression of porcine interferon-α and its bioactivity analysis in vitro and in vivo

Wang, Pengtao; Lu, Xia; Liang, Xiuli; Han, Fangfang; Ren, Haojie; Zhang, Yixuan; Wei, Zhanyong

doi:10.1007/s00449-020-02457-8

Cited by 3 publications

(5 citation statements)

References 27 publications

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“…In previous reports, poIFN-α was expressed in the Pichia pastoris system, the baculovirus system and the E. coli expression system (Mallick et al 2011 ; Huang et al 2012 ; Wang et al 2021 ). For mammalian cell expression systems and Pichia pastoris systems, culture is very time-consuming and expensive, the yield of recombinant proteins is very low, and the purification process is relatively complicated.…”

Section: Discussionmentioning

confidence: 99%

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Analysis of the Differential Expression and Antiviral Activity of Porcine Interferon-α In Vitro

Fang

Zhang

et al. 2023

Int J Pept Res Ther

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Porcine interferon α (poIFN-α) is a crucial cytokine that can prevent and treat viral infections. Seventeen functional porcine IFN-α subtypes were found in the porcine genome. In this study, multiple sequence alignment was performed to analyze IFN-α protein structure and function. Phylogenetic tree analysis of the poIFN gene family defined the evolutionary relationship of various subtypes. PoIFN-αs, including poIFN-α1–17, were expressed in an Escherichia coli expression system. The antiviral activities of these IFN-α proteins against vesicular stomatitis virus (VSV) and pseudorabies virus (PRV) were examined in PK-15 cells. We found that the antiviral activity of different poIFN-α molecules greatly differed as follows: the poIFN-α14 and 17 subtypes had the greatest antiviral activities against VSV and PRV in PK-15 cells, poIFN-α1, 2, 3, and 8 exhibited lower biological activities, and poIFN-α4, 5, 6, 7, 9, 10, 11, 12, 13, and 16 had minimal or no effect in the tested target cell‒virus systems. Moreover, our studies demonstrated that the antiviral activity of IFN-α was positively correlated with the induction of IFN-stimulated genes, such as 2′–5′ oligoadenylate synthetase 1 (OSA1), interferon-stimulated gene 15 (ISG15), myxoma resistance protein 1 (Mx1), and protein kinase R (PKR). Thus, our experimental results provide important information about the antiviral functions and mechanism of poIFN-α.

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Section: Discussionmentioning

confidence: 99%

“…Compared with other expression systems, the E. coli expression systems offer several advantages for protein production, such as high yields, low costs, and rapid expression. Wang et al successfully expressed recombinant poIFN-α with high bioactivity in E. coli (Wang et al 2021 ). PoIFN-α that was cloned and expressed in E. coli.…”

Section: Discussionmentioning

confidence: 99%

Analysis of the Differential Expression and Antiviral Activity of Porcine Interferon-α In Vitro

Fang

Zhang

et al. 2023

Int J Pept Res Ther

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“…On the contrary, little if any activity was shown by IFN-a3, -a7, -a13, -a4 and -a15 [4]. Accordingly, minor differences of the aminoacid sequence can account for dramatic increases in antiviral activity: the mutant PoIFN-α-156s inhibits much more PRV replication in a dose-dependent manner in vitro, compared with the original PoIFN-α [218]. Among IFN-stimulated genes, ISG15 [219] and ISG20 [220] probably play a major role in regulating the antiviral control activities.…”

Section: Ifn Sensitivity Of Prvmentioning

confidence: 99%

The Swine IFN System in Viral Infections: Major Advances and Translational Prospects

Razzuoli

Armando²,

Paolis

et al. 2022

Pathogens

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Interferons (IFNs) are a family of cytokines that play a pivotal role in orchestrating the innate immune response during viral infections, thus representing the first line of defense in the host. After binding to their respective receptors, they are able to elicit a plethora of biological activities, by initiating signaling cascades which lead to the transcription of genes involved in antiviral, anti-inflammatory, immunomodulatory and antitumoral effector mechanisms. In hindsight, it is not surprising that viruses have evolved multiple IFN escape strategies toward efficient replication in the host. Hence, in order to achieve insight into preventive and treatment strategies, it is essential to explore the mechanisms underlying the IFN response to viral infections and the constraints thereof. Accordingly, this review is focused on three RNA and three DNA viruses of major importance in the swine farming sector, aiming to provide essential data as to how the IFN system modulates the antiviral immune response, and is affected by diverse, virus-driven, immune escape mechanisms.

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“…al. 2011;Huang et al 2012;Wang et al 2021). For mammalian cell expression systems and Pichia pastoris systems, culture is very time-consuming and expensive, the yield of recombinant proteins is very low, and the puri cation process is relatively complicated.…”

mentioning

confidence: 99%

“…Compared with other expression systems, E. coli expression systems offer several advantages for protein production, such as high yields, low costs, and rapid expression. Wang et al successfully expressed recombinant porcine IFN-α with high bioactivity in E. coli(Wang et al 2021). Porcine IFN-α was also cloned and expressed in E. coli.…”

mentioning

confidence: 99%

Analysis of the differential expression and antiviral activity of porcine interferon-α in vitro

Fang

Zhang

et al. 2023

Preprint

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Porcine interferon α (poIFN-α) is a crucial cytokine that can prevent and treat viral infection. Seventeen functional porcine IFN-α subtypes were found in the porcine genome. In this study, multiple sequence alignment was performed to analyze IFN-α protein structure and function. Phylogenetic tree analysis of the porcine IFN gene family defined the evolutionary relationship of the various subtypes. Porcine IFN-αs, including porcine IFN-α1–17, were expressed in an E. coli expression system. The antiviral activities of these IFN-α proteins against vesicular stomatitis virus (VSV) and pseudorabies virus (PRV) were examined in PK-15 cells. We found that the antiviral activity of different porcine IFN-α molecules greatly differed: the porcine IFN-α14 and 17 subtypes had the greatest antiviral activities against VSV and PRV in PK-15 cells, porcine IFN-α1, 2, 3, and 8 exhibited lower biological activities, and porcine IFN-α4, 5, 6,7, 9, 10, 11, 12, 13, and 16 had minimal or no effect in the tested target cell‒virus systems. Moreover, our studies demonstrated that the antiviral activity of IFN-α was positively correlated with the induction of IFN-stimulated genes, such as 2’-5’ oligoadenylate synthetase 1 (OSA1), interferon-stimulated gene 15 (ISG15), myxoma resistance protein 1 (Mx1), and Protein kinase R (PKR). Thus, our experimental results provide important information for understanding the antiviral functions and mechanism of porcine IFN-α.

show abstract

Expression of porcine interferon-α and its bioactivity analysis in vitro and in vivo

Cited by 3 publications

References 27 publications

Analysis of the Differential Expression and Antiviral Activity of Porcine Interferon-α In Vitro

Analysis of the Differential Expression and Antiviral Activity of Porcine Interferon-α In Vitro

The Swine IFN System in Viral Infections: Major Advances and Translational Prospects

Analysis of the differential expression and antiviral activity of porcine interferon-α in vitro

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