Expression of pituitary adenylate cyclase activating polypeptide (PACAP) and PACAP type I A receptor mRNAs in granulosa cells of preovulatory follicles of the rat ovary

Koh, Phil-Ok; Kwak, Soo Dong; Kang, Sang Soo; Cho, Gyeong Jae; Chun, Sang-Young; Kwon, Ho; Choi, Wan Sung

doi:10.1002/(sici)1098-2795(200004)55:4<379::aid-mrd4>3.3.co;2-e

Cited by 6 publications

(9 citation statements)

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“…However, the distribution of PACAP receptor types in the ovary among species is variable. Some reports have suggested that either VPAC-1 or PAC-1 alone is found in the rat ovary (Gottschall et al 1990, Scaldaferri et al 1996, whereas others have indicated that PAC-1 and VPAC-2 mRNA, alone or together, are present in rodent granulosa cells (Gras et al 2000, Ko & Park-Sarge 2000, Koh et al 2000, Apa et al 2002. Our data indicate that PAC-1 and VPAC-2 were found in bovine granulosa cells and that these are modulated during the ovulatory process.…”

Section: Regulation Of Pacap In Ovarian Folliclessupporting

confidence: 45%

Gonadotropin-dependent regulation of bovine pituitary adenylate cyclase-activating polypeptide in ovarian follicles prior to ovulation

Sayasith

Brown²,

Sirois³

2007

Reproduction

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To study the regulation of bovine pituitary adenylate cyclase-activating polypeptide (PACAP) in preovulatory follicles prior to ovulation, PACAP cDNA was isolated by RT-PCR. Its open reading frame (ORF) is composed of 531 bp, and encodes for a 176-amino acid protein that bears 76-90% identity with other PACAP homologs. Using bovine preovulatory follicles obtained between 0 and 24 h after human chorionic gonadotropin (hCG) and semiquantitative RT-PCR/Southern blot, we demonstrate that levels of PACAP mRNA were low at 0 h, markedly increased at 6 and 12 h (P!0.05), and declined 18 and 24 h after hCG. Levels of PACAP mRNA were high in the bovine pituitary, testis, intestine and uterus, but moderate to low in other tissues. Analyses performed on isolated preparations of granulosa and theca cells showed a significant increase of PACAP transcripts in both cell types after hCG, whereas primary granulosa cell cultures revealed high levels of PACAP as well as its receptors PAC-1 and VPAC-2 mRNA after forskolin treatment. Overexpression of the catalytic subunit of protein kinase A (PKA) in granulosa cells stimulated, but treatment with H89 or PKA inhibitor protein inhibited PACAP mRNA expression, whereas PACAP overexpression stimulated an increase in abundance of transcripts for PGHS-2, PGES, EP2 receptor, progesterone receptor, and ADAMTS-1, but not for P450-side chain cleavage and P450 aromatase. Thus, this study demonstrates the gonadotropin-dependent regulation of PACAP mRNA in bovine preovulatory follicles, the importance of PKA activation in the expression of PACAP in granulosa cells, and stimulating effect of PACAP on gene expression during the ovulatory process. Reproduction (2007) 133 441-453

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Section: Regulation Of Pacap In Ovarian Folliclessupporting

confidence: 45%

Gonadotropin-dependent regulation of bovine pituitary adenylate cyclase-activating polypeptide in ovarian follicles prior to ovulation

Sayasith

Brown²,

Sirois³

2007

Reproduction

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“…However, after resumption of meiotic maturation, we observed a decrease in the levels of mRNA for these receptors, followed by a decrease in the number of functional receptors on the cell surface, as measured by an inability to stimulate Ca CC mobilization. Therefore, at the time of PACAP production in preovulatory follicles, 3-6 h after the LH surge (Gras et al 1996, Koh et al 2000, this peptide is no longer able to directly interfere with oocyte maturation.…”

Section: Discussionmentioning

confidence: 99%

Characterization and expression of different pituitary adenylate cyclase-activating polypeptide/vasoactive intestinal polypeptide receptors in rat ovarian follicles

Vaccari¹,

Latini²,

Barberi³

et al. 2006

Journal of Endocrinology

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Pituitary adenylate cyclase-activating polypeptide (PACAP) is a bioactive peptide transiently expressed in preovulatory follicles. PACAP acts by interacting with three types of PACAP receptors. PACAP type I receptor (PAC 1 -R), which binds specifically to both PACAPs and vasoactive intestinal polypeptide (VIP), although with lower affinity, and two VIP receptors, VPAC 1 -R and VPAC 2 -R, which bind to PACAP and VIP with equal affinity. In the present study, we showed the expression of all three receptors in whole ovaries obtained from juvenile and gonadotropin-treated immature rats. A more detailed analysis on cells from preovulatory follicles showed that PAC 1 -R and VPAC 2 -R were expressed in granulosa cells, whereas only VIP receptors were expressed in theca/interstitial (TI) cells and fully grown oocytes presented only PAC 1 -R. The distribution of the VIP receptors was confirmed by immunofluorescence. HCG treatment induced stimulation of PAC 1 -R in granulosa cells and VPAC 2 -R in TI cells. The presence of functional PACAP/VIP receptors was also supported by metabolic studies. We further evaluated the presence of PACAP and VIP receptors by testing the effect of these peptides on apoptosis in granulosa cells cultured, isolated or in whole follicles. Treatment of follicles with PACAP and VIP dosedependently inhibited apoptosis, while only PACAP significantly inhibited isolated granulosa cells. These results demonstrate a different expression of PACAP/VIP receptors in the various follicle compartments and suggest a possible role for PACAP and VIP on granulosa and TI cells, both during follicle development and ovulation.

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“…The activities of gonadotropins are modulated by many intraovarian paracrine/autocrine growth factors, such as activin, insulin-like growth factors, epidermal growth factor, and others including PACAP (Danforth 1995, Gräs et al 1996, Ko et al 1999, Lee et al 1999, Koh et al 2000, Ge 2000, Wang et al 2003. Our previous study has shown that exogenous ovine PACAP 38 (1 nM) can stimulate the expression of tpacap 38 in cultured tilapia astrocytes (Lo et al 2007).…”

Section: W-t Huang and Othersmentioning

confidence: 99%

“…In the rat ovary, the inductive effect of gonadotropins on the expression of PACAP and it receptors in granulosa cells in vivo and in vitro has been elucidated (Gräs et al 1996, Ko et al 1999, Lee et al 1999, Ko & Park-Sarge 2000, Koh et al 2000. PACAP stimulates cAMP accumulation and steroidogenesis in a dose-dependent manner from cultured granulosa cells (Zhong & Kasson 1994, Heindel et al 1996.…”

Section: Introductionmentioning

confidence: 99%

Expression and in vitro regulation of pituitary adenylate cyclase-activating polypeptide (pacap38) and its type I receptor (pac1-r) in the gonads of tilapia (Oreochromis mossambicus)

Huang¹,

Li²,

Wu³

et al. 2009

Reproduction

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Pituitary adenylate cyclase-activating polypeptide (PACAP), a pleiotropic neuropeptide, has diverse functions in mammals. However, studies of the expression and function of PACAP and its receptor in fish, particularly in the reproductive system, are still limited. In this report, semi-quantitative RT-PCR and immunohistochemical staining were performed to identify expression domains of commercially important tilapia (Oreochromis mossambicus). PACAP (tpacap 38 ) and its type I receptor (tpac 1 -r). Transcripts were detected in the brain, gallbladder, gill, heart, intestine, kidney, muscles, pancreas, spleen, stomach, testes, and ovaries, but not in the liver. Expression of tpacap 38 and tpac 1 -r mRNA in brain tissue was significantly higher in both sexes compared with other tissues. Addition of exogenous ovine PACAP 38 (0.25-5 nM), cAMP analog (dibutyryl-cAMP, 0.25-1.5 mM) or forskolin (adenylate cyclase activator, 1-10 mM) significantly upregulated tpacap 38 in the gonads via a dose-and time-dependent fashion. This effect reached a maximal level at 2 h after induction, and then decreased with prolonged culture for up to 4 or 8 h. Additionally, the expression levels of tpac 1 -r were not significantly affected by ovine PACAP 38 or dibutyryl-cAMP in either sex. Forskolin had a slightly inductive effect and its function could be suppressed with the addition of protein kinase A (PKA) inhibitor, H89 (10 mM), indicating involvement of the cAMP-PKA signaling pathway in the regulation of tpacap 38 . Expression of tpacap 38 and tpac 1 -r in the gonads of tilapia suggests that PACAP may mediate gonadotropin action via paracrine/autocrine mechanisms in this bony fish.

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Expression of pituitary adenylate cyclase activating polypeptide (PACAP) and PACAP type I A receptor mRNAs in granulosa cells of preovulatory follicles of the rat ovary

Cited by 6 publications

References 0 publications

Gonadotropin-dependent regulation of bovine pituitary adenylate cyclase-activating polypeptide in ovarian follicles prior to ovulation

Gonadotropin-dependent regulation of bovine pituitary adenylate cyclase-activating polypeptide in ovarian follicles prior to ovulation

Characterization and expression of different pituitary adenylate cyclase-activating polypeptide/vasoactive intestinal polypeptide receptors in rat ovarian follicles

Expression and in vitro regulation of pituitary adenylate cyclase-activating polypeptide (pacap38) and its type I receptor (pac1-r) in the gonads of tilapia (Oreochromis mossambicus)

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