Expression of pituitary adenylate cyclase-activating polypeptide mRNA in the cochlea of rats

Kawano, Hirokazu; Shimozono, Masami; Tono, Tetsuya; Miyata, Atsuro; Komune, Shizuo

doi:10.1016/s0169-328x(01)00241-8

Cited by 14 publications

(14 citation statements)

References 16 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The morphology of the inner ear showed no differences between the homozygous PACAP-defi cient and WT mice. Our research group found PAC1-R immunopositivity in both inner and outer hair cells, outer phalangeal cells (Deiters' cells), and pillar cells in accordance with the previous results of Kawano et al [ 3 ], AbuHamdan et al [ 1 ] and Drescher et al [ 2 ]. The localization of PAC1-R immunoreactivity did not show any differences between homozygous PACAP-defi cient and WT mice, but in PACAP-defi cient mice the hair cells and outer phalangeal cells showed lower intensity of PAC1-R immunoreactivity compared to WT mice.…”

Section: Pacap and Pac1-r In The Cochleasupporting

confidence: 93%

“…Strong PACAP immunostaining was found in the cochlear nerve in the axons of afferents leaving the spiral ganglion ( Fig. 31.1 ), corresponding to previous PACAP preprotein mRNA fi ndings of Kawano et al [ 3 ]. Although type I axons had high immunoreactivity the cell bodies showed low density of immunoreactivity which suggests afferent axonal transport of PACAP in these cells, presumably to modulate excitatory amino acid release at the axon terminals of the afferent cells in the cochlear nucleus.…”

Section: Pacap and Pac1-r In The Cochleasupporting

confidence: 80%

“…The immunoreactivity increased from basal to apical turns. Immunoreactivity is shown in the basolateral extension of marginal cells as predicted from earlier in situ hybridization of the mRNA of PACAP preprotein by Kawano et al [ 3 ]. No PACAP immunoreactivity was detected in the apical membrane of the marginal cells, basal cells, capillaries, nor in fi broblasts of the spiral ligament.…”

Section: Pacap and Pac1-r In The Cochleasupporting

confidence: 65%

“…31.5 ). Therefore, these cells could serve as the origin of PACAP found in the cochlea by Kawano et al [ 3 ]. PACAP was found in the medial olivocochlear efferents, which target the outer hair cells, but not in the lateral olivocochlear efferents, which target the inner hair cells.…”

Section: Pacap In the Brainstem And Thalamusmentioning

confidence: 92%

“…Kawano et al [ 3 ] studied the expression of PACAP mRNA in the cochlea of Wistar rats using RT-PCR and in situ hybridization techniques. The RT-PCR primers designed against PACAP mRNA yielded the expected 553 bp RNA sequence.…”

Section: Pacap and Pac1-r In The Cochleamentioning

confidence: 99%

See 4 more Smart Citations

Pituitary Adenylate Cyclase-Activating Polypeptide in the Auditory System

Fülöp

Reglődi

Németh

et al. 2016

Current Topics in Neurotoxicity

View full text Add to dashboard Cite

Pituitary adenylate cyclase-activating polypeptide (PACAP) is a neuropeptide with well-known neurotrophic, neuroprotective, antioxidant, and antiapoptotic effects. It also exerts protective effects in sensory organs, such as visual and olfactory system. In this review we present data regarding the localization and effects of PACAP in the auditory system. PACAP and its specifi c receptor (PAC1-R) are present in the organ of Corti in hair cells, supporting cells, and different nerve fi bers. They are also present in the spiral ganglion showing co-localization with efferent fi bers of glutamatergic and adrenergic pathways, probably directly affecting the efferent signal transduction in the inner ear. PACAP and its specifi c receptor also occur in the stria vascularis suggesting a role in endolymph production; furthermore, they are present in central pathways of the auditory system such as the cochlear nuclei, superior olivary complex, inferior colliculus, and medial geniculate body. PAC1-receptor is also present in the inner ear in PACAP-defi cient mice, occurring at the same localization but with altered expression compared to wildtype mice. PACAP protects hair cells from H 2 O 2 -induced apoptosis in chicken inner ear cell cultures in vitro, and the lack of PACAP affects the Ca 2+ -binding protein expression in hair cells in PACAP-defi cient mice under control circumstances and after ototoxic drug treatment.

show abstract

Section: Pacap and Pac1-r In The Cochleasupporting

confidence: 93%

Section: Pacap and Pac1-r In The Cochleasupporting

confidence: 80%

Section: Pacap and Pac1-r In The Cochleasupporting

confidence: 65%

Section: Pacap In the Brainstem And Thalamusmentioning

confidence: 92%

Section: Pacap and Pac1-r In The Cochleamentioning

confidence: 99%

See 3 more Smart Citations

Pituitary Adenylate Cyclase-Activating Polypeptide in the Auditory System

Fülöp

Reglődi

Németh

et al. 2016

Current Topics in Neurotoxicity

View full text Add to dashboard Cite

show abstract

Neurochemistry of Olivocochlear Neurons in the Hamster

Reuss

Disque-Kaiser

Antoniou-Lipfert

et al. 2009

The Anatomical Record

View full text Add to dashboard Cite

The present study was conducted to characterize the superior olivary complex (SOC) of the lower brain stem in the pigmented Djungarian hamster Phodopus sungorus. Using Nissl-stained serial cryostat sections from fresh-frozen brains, we determined the borders of the SOC nuclei. We also identified olivocochlear (OC) neurons by retrograde neuronal tracing upon injection of Fluoro-Gold into the scala tympani. To evaluate the SOC as a putative source of neuronal nitric oxide synthase (nNOS), arginine-vasopressin (AVP), oxytocin (OT), vasoactive intestinal polypeptide (VIP), or pituitary adenylate cyclase-activating polypeptide (PACAP) that were all found in the cochlea, we conducted immunohistochemistry on sections exhibiting retrogradely labeled neurons. We did not observe AVP-, OT-, or VIP-immunoreactivity, neither in OC neurons nor in the SOC at all, revealing that cochlear AVP, OT, and VIP are of nonolivary origin. However, we found nNOS, the enzyme responsible for nitric oxide synthesis in neurons, and PACAP in neuronal perikarya of the SOC. Retrogradely labeled neurons of the lateral olivocochlear (LOC) system in the lateral superior olive did not contain PACAP and were only infrequently nNOS-immunoreactive. In contrast, some shell neurons and some of the medial OC (MOC) system exhibited immunofluorescence for either substance. Our data obtained from the dwarf hamster Phodopus sungorus confirm previous observations that a part of the LOC system is nitrergic. They further demonstrate that the medial olivocochlear system is partly nitrergic and use PACAP as neurotransmitter or modulator. Anat Rec, 292:461-471, 2009.

show abstract

The disablement process in rheumatoid arthritis

Escalante¹,

Rincón²

2002

Arthritis & Rheumatism

View full text Add to dashboard Cite

Expression of pituitary adenylate cyclase-activating polypeptide mRNA in the cochlea of rats

Cited by 14 publications

References 16 publications

Pituitary Adenylate Cyclase-Activating Polypeptide in the Auditory System

Pituitary Adenylate Cyclase-Activating Polypeptide in the Auditory System

Neurochemistry of Olivocochlear Neurons in the Hamster

The disablement process in rheumatoid arthritis

Contact Info

Product

Resources

About