Expression of Photosynthesis-Related Genes during the Leaf Development of a C₃Plant Rice as Visualized byIn SituHybridization

Gene Expression and Accumulation of Rubisco in Bundle Sheath and Mesophyll Cells during Leaf Development and Senescence in Rice, a C3Plant

“…2B, C). The results of the expression of rbcS are consistent with our previous report (Tsutsumi et al, 2006). These results indicate that the expression of rbcS in bundle sheath cells decreased earlier than that in mesophyll cells during leaf development while the expression of rbcL continued in both bundle sheath and mesophyll cells.…”

“…These results indicate that the expression of rbcL decreased at a similar rate in both bundle sheath and mesophyll cells during leaf senescence while the expression of rbcS in bundle sheath cells decreased faster during leaf senescence. No signal was observed in bundle sheath and mesophyll cells when sense probes were used as in our previous report (Tsutsumi et al, 2006) (data not shown).…”

“…Our previous report showed that rbcS and cab were expressed in both bundle sheath and mesophyll cells in the basal region of the emerging rice leaves while both genes were expressed only in mesophyll cells in tip and middle regions (Tsutsumi et al, 2006). This fact suggests that the expression of photosynthesisrelated genes in emerging rice leaves is less tissue specific at very early stage but gradually becomes mesophyll-specifi c during leaf development.…”

“…RNA probe for rbcS was produced as described in Tsutsumi et al (2006). As the template for rbcL RNA probe, the rice cDNA clone (accession number AK105600) inserted in pME18SFL-3 was used (provided by National Institute of Agrobiological Sciences).…”

“…We previously examined the expression of photosynthetic genes, rbcS and cab, which encode the small subunit of ribulose-1,5-bisphosphate carboxylase/oxygenase (Rubisco) and light har vesting chlorophyll a/b binding protein, respectively, in the emerging leaves and lamina joint of rice (Tsutsumi et al, 2006(Tsutsumi et al, , 2007. The photosynthetic genes were expressed in both bundle sheath and mesophyll cells in the immature basal region of the emerging leaves whereas the genes were expressed only in mesophyll cells in more mature middle and tip regions.…”