Expression of phosphatase and tensin homolog deleted on chromosome ten in liver of athymic mice with hepatocellular carcinoma and the effect of Fuzheng Jiedu Decoction

Abstract

AIM:To explore the expression of phosphatase and tensin homolog deleted on chromosome ten (PTEN) in liver of athymic mice with hepatocellular carcinoma (HCC) and the effect of Fuzheng Jiedu Decoction (FJD). METHODS:Forty eight male BALB/c athymic mice models were built by Bel-7402 with an indirect method. After 24 h of postoperation, the 48 athymic mice were distributed randomly into 4 groups: A, B, C, D, each group had 12 athymic mice. Group A were treated by intragastric administration with FT207 (Tegafur) for 4 wk. Group B, C and D were treated by intragastric administration with FJD (complex prescription of Chinese crude drug) that had been delegated into 3 kinds of density as the low, middle, and high for 4 wk. At last, athymic mice were put to death, live time, volume of tumors, exponent of tumors and the tumor metastasis in livers were observed; and PTEN was detected in hepatic tissue, latero-cancer tissue and cancer tissue by immunohistochemistry. RESULTS:Four weeks later, the total survival rate in treatment group (A + B + C) was 50% and higher than the control group (0%) treated by FT207, (P < 0.01). The survival rate in group A, B, C was higher than in group D, and except group A with D, there was significant differences (Fisher's Exact Test P = 0.05 or 0.01). And no differences were observed between the treatment groups and the control group in volume of tumors and exponent of tumors (P > 0.05). Tumor metastasis in livers of the treatment group was less than the controls ( F i s h e r 's E x a c t Te s t , P = 0 . 0 2 1 ) . T h e r e s u l t o f immunohistochemistry showed that the intensity of PTEN in latero-cancer tissue was the highest, and then the hepatic tissue, the lowest was cancer tissue (KruskalWallis test, χ 2 = 60.67, P = 0.000). It also showed that the intensity of PTEN in treatment groups (A, B, C) was higher than the control group (D) (F = 5.90, P = 0.002 in hepatic tissue and F = 15.99, P = 0.000 in latero-cancer tissue and χ 2 = 26.08, P = 0.000 in cancer tissue), and group B is the highest in the treatment groups (P < 0.05, r = 0.01. respectively). However, there was no significant statistic difference between group A and group C (P > 0.05).

Effect of the herbal formulation Jianpijiedu on the TCRVβCDR3 repertoire in rats with hepatocellular carcinoma and subjected to food restriction combined with laxative

“…According to the theory of traditional Chinese medicine, JPJD exerts certain effects, including enhancement of the absorption and transportation of nutrient substances and an antitumor effect ( 2 ). However, previous studies have indicated that JPJD can effectively promote quality of life and survival time, but is not able to directly inhibit tumor growth ( 2 , 3 ).…”

“…According to the theory of traditional Chinese medicine, JPJD exerts certain effects, including enhancement of the absorption and transportation of nutrient substances and an antitumor effect ( 2 ). However, previous studies have indicated that JPJD can effectively promote quality of life and survival time, but is not able to directly inhibit tumor growth ( 2 , 3 ). Therefore, a hepatocellular carcinoma model based on food restriction combined with laxative (FRL) administration was established in the present study in order to investigate the immunological mechanism underlying the potential anti-hepatoma effects of JPJD with TCRVβCDR3 as the suggested target.…”

“…A randomized clinical trial of patients with hepatocellular carcinoma has shown that this formulation can effectively promote quality of life and liver function if combined with intra-arterial chemotherapy ( 1 ). Furthermore, studies have indicated that JPJD can improve survival time, reduce pulmonary metastasis ( 2 ) and maintain the body and visceral weight of nude mice transplanted with human hepatocellular carcinoma tissue ( 3 ). These previous findings indicate that JPJD may be able to improve digestive and absorptive functions and the quality of life in patients with hepatocellular carcinoma.…”

The study on mechanism of the modified Chinese herbal compound, jianpijiedu, on a mouse model of hepatic carcinoma cachexia

“…It has been previously reported that CC may affect >80% of patients with cancer, which directly affects the overall survival and the life quality of the patients, reduces the tolerance to radiotherapy and chemotherapy, and increases the occurrence rate of complications, thus CC is regarded as an crucial cause of death in patients with cancer ( 3 , 4 ). The pathogenesis of CC remains complex, and there is little information regarding the use of therapeutic strategies including anti-inflammatories, appetite stimulation and nutritional support, which may aid in the prevention of skeletal muscle degeneration, however further investigation is required ( 5 , 6 ).…”

The Chinese HerbJianpijieduContributes to the Regulation of OATP1B2 and ABCC2 in a Rat Model of Orthotopic Transplantation Liver Cancer Pretreated with Food Restriction and Diarrhea

“…Our recent studies [ 18 ] revealed that OATP2A1 played a pivotal role in the development of liver cancer. The general status of liver cancer patients was improved by [ 19 ] Jianpijiedu decoction (JPJD). However, further research is needed to determine whether JPJD positively impacts the liver microenvironment during liver cancer by regulating the levels of OATP1B2 or ABCC2.…”