Expression of pheromone biosynthesis activating neuropeptide and its receptor (PBANR) mRNA in adult female Spodoptera exigua (Lepidoptera: Noctuidae)

Abstract

The full-length cDNA of pheromone biosynthesis activating neuropeptide receptor (PBANR) was cloned from the beet armyworm, Spodoptera exigua (Hübner) (Lepidoptera: Noctuidae); it included an open reading frame of 1,053 bp encoding 350 amino acids. The PBANR of S. exigua (SePBANR) was structurally characteristic of G protein-coupled receptor and its amino acid sequence shared 98% identity with the PBANR of Spodoptera littoralis. Both pheromone biosynthesis activating neuropeptide (PBAN) and PBANR mRNA abundance were measured in the brain-subesophageal ganglion complex, pheromone gland, ventral nerve cord, and ovary of S. exigua female moths by real-time RT-PCR. The abundance of PBAN mRNA in brain-subesophageal ganglion complex and PBANR mRNA in pheromone gland was significantly greater compared to other tissues, suggesting that the ligand-receptor relationship of PBAN and PBANR exists quantitatively in S. exigua. Both PBAN and PBANR expression displayed a remarkable diurnal rhythm, for they were low and stable during the photophase (07:00-21:00) and increased markedly during the scotophase (with a maximum abundance at 23:30) in 3-day-old female moths. The abundance of PBAN and PBANR increased steadily from the 1st day to the 5th day of the adult female life. The pattern of both diurnal and daily expression of PBAN and PBANR mRNA were coincident with enhanced capacity of sex pheromone release and mating of S. exigua moths during the same period. We infer from these results that pheromone biosynthesis and release in S. exigua is regulated by PBAN via up-regulating synthesis.

RNA interference of pheromone biosynthesis-activating neuropeptide receptor suppresses mating behavior by inhibiting sex pheromone production in Plutella xylostella (L.)

“…was detected from neuronal tissues such as brain, ventral nerve cord, thoracic ganglion as well as male aedaegus, homologous to female pheromone gland (Rafaeli et al, 2007). PBANr expression from Spodoptera exigua was found in suboesophageal ganglion, ventral nerve cord, pheromone gland, and ovary (Cheng et al, 2010). Relative PBANr expression level of neuronal tissue was much lower than that of pheromone gland in Helicoverpa spp.…”

“…However, other reports suggest that other neuronal tissue also can be the target of PBAN (Rafaeli et al, 2007;Cheng et al, 2010). PBANr transcript from female Helicoverpa spp.…”

“…Relative PBANr expression level of neuronal tissue was much lower than that of pheromone gland in Helicoverpa spp. and S. exigua (Rafaeli et al, 2007;Cheng et al, 2010). Multiple PBAN receptor transcripts (Hev-PBANr-A, -B, and -C) resulting from alternative splicing were identified from H. virescens in larval central nervous system (Kim et al, 2008).…”

Cloning of the heat shock protein 90 and 70 genes from the beet armyworm, Spodoptera exigua, and expression characteristics in relation to thermal stress and development

“…The reference gene of β-actin was used as an endogenous control when quantification of target genes was determined. This gene is an appropriate control in this insect during this wide developmental window as determined in our previous studies (Tian et al 2009;Cheng et al 2010). The average concentration across replicates was obtained from the standard curves and the relative expression ratio of each target gene was calculated according to protocols established by the manufacturer (ABI Prism 7700 Sequence Detection System).…”

Identification of Functionally Important Residues of the Silkmoth Pheromone Biosynthesis-activating Neuropeptide Receptor, an Insect Ortholog of the Vertebrate Neuromedin U Receptor

“…The PBAN receptor (PBANR) was first cloned from H. zea (20) as an ortholog of the vertebrate neuromedin U receptor (NMUR) (21)(22)(23)(24), which recognizes peptides with a C-terminal FRPRN-NH 2 motif such as neuromedin U (NMU) and neuromedin S (25,26). Since then, PBANRs from B. mori (27), Heliothis virescens (28), Manduca sexta (GenBank TM accession numbers FJ240221 to FJ240224), 5 Plutella xylostella (29), Helicoverpa armigera (30), Spodoptera littoralis (31), Spotoptera exigua (32), Pseudaletia separata (33), and Ostrinia nubilalis (34) have been identified. Functional analyses have shown that PBANR activation triggers an influx of extracellular Ca 2ϩ in isolated B. mori pheromone glands (35) as well as insect cells transiently expressing PBANR (20,27).…”