Expression of peroxiredoxin I regulated by gonadotropins in the rat ovary

Lee, Yu-Il; Kang, Woodae; Kim, Miyoung; Cho, Moon-Kyoung; Chun, Sang-Young

doi:10.5653/cerm.2011.38.1.18

Cited by 5 publications

(11 citation statements)

References 37 publications

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“…Peroxiredoxins 1 and 2 were highly expressed in ovary and testis [47], [52]. In the female, peroredoxin 1 gene expresses in 3-day-old follicles and increases its expression in 21-day-old during folliculogenesis in the rat [53]. In addition to being found in human endometrium and cervix-vagina fluid [54] and in the epithelium and the endometrial stroma of the uterus, peroredoxins 2 has been reported to be present in the mouse ooplasm of primary follicles, secondary follicles or premature follicles [55].…”

Section: Discussionmentioning

confidence: 96%

Proteomic Profiling of Rabbit Embryonic Stem Cells Derived from Parthenotes and Fertilized Embryos

et al. 2013

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Rabbit embryonic stem (rES) cells can be derived from various sources of embryos. However, understanding of the gene expression profile, which distincts embryonic stem (ES) cells from other cell types, is still extremely limited. In this study, we compared the protein profiles of three independent lines of rabbit cells, i.e., fibroblasts, fertilized embryo-derived stem (f-rES) cells, and parthenote-derived ES (p-rES) cells. Proteomic analyses were performed using two-dimensional gel electrophoresis (2-DE) and mass spectrometry. Collectively, the expression levels of 100 out of 284 protein spots differed significantly among these three cell types (p<0.05). Of those differentially expressed spots, 91% were identified in the protein database and represented 63 distinct proteins. Proteins with known identities are mainly localized in the cytoplasmic compartments (48%), nucleus (14%), and cytoskeletal machineries (13%). These proteins were majorly involved in biological functions of energy and metabolic pathways (25%), cell growth and maintenance (25%), signal transduction (14%), and protein metabolisms (10%). When protein expression levels among cell types were compared, six proteins associated with a variety of cellular activities, including structural constituents of the cytoskeleton (tubulins), structural molecule (KRT8), catalytic molecules (α-enolase), receptor complex scaffold (14-3-3 protein sigma), microfilament motor proteins (Myosin-9), and heat shock protein (HSP60), were found highly expressed in p-rES cells. Two proteins related to HSP activity and structural constituent of cytoskeleton in f-rES cells, and one structural molecule activity protein in fibroblasts showed significantly higher expression levels (p<0.05). Marker protein expressions in f-rES and p-rES cells were further confirmed by Western blotting and immunocytochemical staining. This study demonstrated unique proteomic profiles of the three rabbit cell types and revealed some novel proteins differentially expressed between f-rES and p-rES cells. These analyses provide insights into rES cell biology and would invite more in-depth studies toward rES cell applications.

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Section: Discussionmentioning

confidence: 96%

Proteomic Profiling of Rabbit Embryonic Stem Cells Derived from Parthenotes and Fertilized Embryos

et al. 2013

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“…The mechanisms behind ischemic injury involve energy depletion and oxidative stress, which produces reactive oxygen species, such as hydroxyl radicals, superoxide anion, and hydrogen peroxide. This can eventually cause damage to lipids, DNA, enzymes and structural proteins, leading to follicular atresia [ 15 ]. The PRDX1 belongs to a six-member family of peroxidases involved in antioxidant defenses and intracellular signaling, and are present in granulosa cells [ 15 ].…”

Section: Discussionmentioning

confidence: 99%

“…This can eventually cause damage to lipids, DNA, enzymes and structural proteins, leading to follicular atresia [ 15 ]. The PRDX1 belongs to a six-member family of peroxidases involved in antioxidant defenses and intracellular signaling, and are present in granulosa cells [ 15 ]. In the present study the PRDX1 gene was up-regulated in xenografts, probably as a physiological response to reduce hydrogen peroxide and alkyl hydroperoxides.…”

Section: Discussionmentioning

confidence: 99%

“…Up to 5 days after transplantation, Van Eyck et al (2010)[ 13 ] identified a hypoxic period; however, more studies should be performed to investigate the subsequent period. As this time point is critical to graft development, the balance in the expression of anti-apoptotic genes (B-cell lymphoma 2— BCL2 ) and pro-apoptotic genes (BCL2-Associated X Protein– BAX ; [ 14 ]) and oxidative stress markers (peroxiredoxin-1— PRDX1 ; [ 15 ]), as well as the expression pattern of other genes used as markers of follicular function, such as follicle-stimulating hormone receptor ( FSHR) , insulin-like growth factor 1 and 2 receptors ( IGF1R and IGF2R ; [ 16 ]) may also be important to evaluate the graft establishment and development. Thus, the aim of this study was to evaluate, using the bovine graft model and in a short time after xenotransplantation, several functional aspects of the grafts, such as ( i ) folliculogenesis maintenance, ( ii ) graft histological components, ( iii ) blood perfusion, ( iv ) gene expression, ( v ) number of host-derived macrophage and neutrophil, ( vi ) and developmental competence after in vitro fertilization of xenograft-derived oocytes.…”

Section: Introductionmentioning

confidence: 99%

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Ovarian Grafts 10 Days after Xenotransplantation: Folliculogenesis and Recovery of Viable Oocytes

et al. 2016

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Ovarian xenotransplantation is a promising alternative to preserve fertility of oncologic patients. However, several functional aspects of this procedure remained to be addressed. The aim of this study was evaluate the feasibility of xenotransplantation as a strategy to maintain bovine ovarian grafts and produce oocytes. Adult ovarian cortical pieces were xenotransplanted to the dorsal subcutaneous of female NOD-SCID mice (n = 62). Grafts were recovered ten days after xenotransplantation. Host and graft weights; folliculogenesis progression; blood perfusion, relative gene expression and number of macrophage and neutrophil of xenografts; in vitro developmental competence of graft-derived oocytes were evaluated. Folliculogenesis was supported in the grafts, as indicated by the presence of primordial, primary, secondary, antral, and atretic follicles. The xenografts showed a greater volumetric density of atretic follicles and higher hyperemia and number of host-derived macrophage and neutrophil (P<0.05), when compared to non-grafted fragments. There was a higher blood perfusion under the back skin in the transplantation sites of host animals than in control and non-grafted (P<0.01). BAX and PRDX1 genes were up-regulated, while BCL2, FSHR, IGF1R and IGF2R were down-regulated, when compared to the control (P<0.01). Twenty seven oocytes were successfully harvested from grafts, and some of these oocytes were able to give rise to blastocysts after in vitro fertilization. However, cleavage and blastocyst rates of xenograft derived oocytes were lower than in control (P<0.01). Despite showing some functional modifications, the ovarian xenografts were able to support folliculogenesis and produce functional oocytes.

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“…PRDX 1–6 are categorized as typical 2-Cys peroxiredoxins, which are distinct from the atypical 2-Cys (PRDX5) and 1-Cys peroxiredoxins (PRDX 6) [ 8 ]. Among these PRDXs, PRDX1 is localized in the cytosol, where it interacts with various signaling molecules [ 9 , 10 ]. In addition, a relationship between granulosa cell stimulation and PRDX1 expression has been previously detected in the rat ovary [ 10 ].…”

Section: Introductionmentioning

confidence: 99%

Peroxiredoxin I maintains luteal function by regulating unfolded protein response

Park

Lee

Seong

et al. 2018

Reprod Biol Endocrinol

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BackgroundMounting evidence shows that ROS regulation by various antioxidants is essential for the expression of enzymes involved in steroidogenesis and maintenance of progesterone production by the corpus luteum (CL). However, the underlying mechanisms of peroxiredoxin 1 (PRDX1), an antioxidant enzyme, in luteal function for progesterone production in mice have not been reported. The aim of this study was to evaluate the functional link between PRDX1 and progesterone production in the CL of Prdx1 knockout (K/O) mice in the functional stage of CL.MethodsThe expression pattern of the unfolded protein response (UPR) signaling pathways, endoplasmic reticulum (ER) stress-induced apoptosis related genes and peroxiredoxins 1 (PRDX1) were investigated by western blotting analysis in CL tissue of 10 weeks mice during functional stage of CL. The protein levels of these genes after ER-stress inducer tunicamycin (Tm), ER-stress inhibitor tauroursodeoxycholic acid (TUDCA) and ROS scavenger, N-acetylcysteine (NAC) stimulation by intraperitoneal (i.p) injection were also investigated in CL tissue of wild type (WT) mice. Finally, we examined progesterone production and UPR signaling related gene expression in CL tissue of Prdx1 K/O mice.ResultsWe demonstrated that PRDX1 deficiency in the functional stage activates the UPR signaling pathways in response to ER stress-induced apoptosis. Interestingly, CL number, serum progesterone levels, and steroidogenic enzyme expression in Prdx1 K/O mice decreased significantly, compared to those in wild type mice. Levels of UPR signaling pathway markers (GRP78/BIP, P50ATF6, and phosphorylated (p)-eIF2) and ER-stress associated apoptotic factors (CHOP, p-JNK, and cleaved caspase-3) were dramatically increased in the CL tissue of Prdx1 K/O mice. In addition, administration of the NAC, reduced progesterone production and activated ER-stress-induced UPR signaling in the CL tissue obtained from the ovary of Prdx1 K/O mice. Taken together, these results indicated that reduction in serum progesterone levels and activation of ER-stress-induced UPR signaling are restored by NAC injection in the CL of Prdx1 K/O mice.ConclusionThese observations provide the first evidence regarding the basic mechanisms connecting PRDX1 and progesterone production in the functional stage of CL.Electronic supplementary materialThe online version of this article (10.1186/s12958-018-0396-0) contains supplementary material, which is available to authorized users.

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Expression of peroxiredoxin I regulated by gonadotropins in the rat ovary

Cited by 5 publications

References 37 publications

Proteomic Profiling of Rabbit Embryonic Stem Cells Derived from Parthenotes and Fertilized Embryos

Proteomic Profiling of Rabbit Embryonic Stem Cells Derived from Parthenotes and Fertilized Embryos

Ovarian Grafts 10 Days after Xenotransplantation: Folliculogenesis and Recovery of Viable Oocytes

Peroxiredoxin I maintains luteal function by regulating unfolded protein response

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