Expression of periostin in the serum of NSCLC and its function on proliferation and migration of human lung adenocarcinoma cell line (A549) in vitro

Abstract

Periostin is over expressed in many epithelial malignant cancers, including lung cancer, breast cancer, ovarian cancer and colon cancer. It is related with the progression and migration of breast and ovarian cancer cells in vitro. The aim of this study was to investigate the serum level of periostin in non-small cell lung cancer (NSCLC) and its relationship with established biological and prognostic factors by enzyme-linked-immunosorbent serologic assay. We also observe the function of periostin on the proliferation and migration of human lung adenocarcinoma cell line (A549) and discuss the mechanism. The mean value for serum periostin (POSTN) was elevated in NSCLC patients (242.84 + or - 5.33 pg/ml) compared to the normal healthy volunteers (215.66 + or - 11.67 pg/ml) (p = 0.030). The serum level of periostin of NSCLC patients had no connection with gender, age, pathological type, TNM stage, lymph node status, tumor size and invasiveness. We constructed a plasmid named pEGFP-N1/POSTN expressing full-length human periostin. Transfecting the plasmid to A549 cells and periostin was efficiently expressed in transfected A549 cells. Our data showed that periostin could promote the proliferation and migration of A549 cells by inducing vimentin and N-cadherin expression and downregulating E-cadherin expression. These results strongly suggest that periostin is a novel molecular which play an important role during the progression and development of NSCLC.

The role of periostin in neoplastic processes

“…In addition, POSTN concentration was evaluated in the serum of and compared to that of a healthy group. Consistent with the results obtained by Sasaki et al [27], in NSCLC patients no significant correlations between the serum concentration of POSTN and patients' gender, age, pathological type, TNM stage, lymph node status, tumor size (G) and invasiveness were found [61]. However, in a more recent report, the same group showed significantly increased expression of POSTN on the protein, but not at the mRNA, level in NSCLC patients compared to the normal lung tissue with high POSTN immunoreactivity in the mesenchymal areas, but not in the cancer cells [62].…”

“…Increased expression of POSTN has been observed in various tumor types, including breast [44,48,[56][57][58], NSCLC [27,49,[59][60][61][62], colorectal [19,29,63], stomach cancers [64][65][66][67][68], pancreatic [32,69], prostate [70][71][72][73][74][75], ovarian cancer [76][77][78][79], and glioblastomas [80][81][82] (Table 1).…”

“…As with other cancers of epithelial origin, non-small--cell lung cancer (NSCLC) was examined for POSTN expression [27,49,59,61,62].…”

“…Using the technique of laser capture microdissection (LCM), the authors established that both tumor epithelia and stromal cells can be a source of POSTN production in NSCLC [60]. It was shown that POSTN may promote proliferation and migration of A549 lung cancer cells by inducing the expression of vimentin and N-cadherin, key mesenchymal cell markers of epithelial-mesenchymal transition (EMT), and downregulating E-cadherin expression [61]. In addition, POSTN concentration was evaluated in the serum of and compared to that of a healthy group.…”

Opposite regulation of epithelial-to-mesenchymal transition and cell invasiveness by periostin between prostate and bladder cancer cells

“…Isolated RNA was used for firststrand cDNA synthesis with Superscript II (Invitrogen) and an oligo-dT primer for 50 min at 42˚C. The cDNA was PCRamplified by Taq DNA polymerase (Takara, Otsu, Japan) with primers specific for E-cadherin Type I, 5'-CGACCCAACCC AAGAATCTA-3' (forward) and 5'-AGGCTGTGCCTTCCT ACAGA-3' (reverse); E-cadherin Type II, 5'-GCCAGGTATGA GATCGTGGT-3' (forward) and 5'-GTGTCTTCAGGCACG ACAAA-3' (reverse) (16). PCR reaction was performed with 30 cycles consisting of denaturation at 94˚C for 30 sec, annealing at 55˚C for 30 sec, and extension at 72˚C for 1 min, followed by a final extension for 7 min.…”