Expression of PEI-coated gold nanoparticles carrying exogenous gene in periwinkle mesophyll cells and its practice in huanglongbing research

Zhang, Junzhe; Lei, Wenxue; Yang, Meng; Zhou, Chengqian; Zhang, Baihong; Yuan, Jun; Wang, Min; Xu, Dongqing; Meng, Xiangchun; Chen, Wenli

doi:10.1016/j.isci.2022.104479

Cited by 5 publications

(1 citation statement)

References 43 publications

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“…Furthermore, PEI or PEI-coated nanomaterials can cross plant cellular barriers and localize in the cytosol or organelles, as it was demonstrated both in vitro , and in vivo studies. Particularly, PEI has been used as carrier to deliver plasmids to plants for transient gene expression, as well as to deliver small interfering RNA, siRNA, for gene silencing . PEI’s high positive charge condensates negatively charged polynucleotides and, at the same time, protects them from degradation, thus improving transfection efficiency.…”

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confidence: 99%

Chitosan-Modified Polyethyleneimine Nanoparticles for Enhancing the Carboxylation Reaction and Plants’ CO₂Uptake

et al. 2023

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Increasing plants' photosynthetic efficiency is a major challenge that must be addressed in order to cover the food demands of the growing population in the changing climate. Photosynthesis is greatly limited at the initial carboxylation reaction, where CO 2 is converted to the organic acid 3-PGA, catalyzed by the RuBisCO enzyme. RuBisCO has poor affinity for CO 2 , but also the CO 2 concentration at the RuBisCO site is limited by the diffusion of atmospheric CO 2 through the various leaf compartments to the reaction site. Beyond genetic engineering, nanotechnology can offer a materials-based approach for enhancing photosynthesis, and yet, it has mostly been explored for the light-dependent reactions. In this work, we developed polyethyleneimine-based nanoparticles for enhancing the carboxylation reaction. We demonstrate that the nanoparticles can capture CO 2 in the form of bicarbonate and increase the CO 2 that reacts with the RuBisCO enzyme, enhancing the 3-PGA production in in vitro assays by 20%. The nanoparticles can be introduced to the plant via leaf infiltration and, because of the functionalization with chitosan oligomers, they do not induce any toxic effect to the plant. In the leaves, the nanoparticles localize in the apoplastic space but also spontaneously reach the chloroplasts where photosynthetic activity takes place. Their CO 2 loading-dependent fluorescence verifies that, in vivo, they maintain their ability to capture CO 2 and can be therefore reloaded with atmospheric CO 2 while in planta. Our results contribute to the development of a nanomaterials-based CO 2 -concentrating mechanism in plants that can potentially increase photosynthetic efficiency and overall plants' CO 2 storage.

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confidence: 99%

Chitosan-Modified Polyethyleneimine Nanoparticles for Enhancing the Carboxylation Reaction and Plants’ CO₂Uptake

et al. 2023

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Three new discovery effector proteins from Candidatus Liberibacter asiaticus psy62 inhibit plant defense through interaction with AtCAT3 and AtGAPA

Li,

Guo,

Zhou

et al. 2024

Plant Cell Rep

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ATG6 interacting with NPR1 increasesArabidopsis thalianaresistance toPstDC3000/avrRps4by increasing its nuclear accumulation and stability

Zhang,

Huang,

Guo

et al. 2024

Preprint

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Autophagy-related gene 6 (ATG6) plays a crucial role in plant immunity. Nonexpressor of pathogenesis-related genes1 (NPR1) acts as a signaling hub of plant immunity. However, the relationship between ATG6 and NPR1 is unclear. Here, we find that ATG6 directly interacts with NPR1.ATG6overexpression significantly increased nuclear accumulation of NPR1. Furthermore, we demonstrate thatATG6increases NPR1 protein levels and improves its stability. Interestingly, ATG6 promotes the formation of SINCs (SA-induced NPR1 condensates)-like condensates. Additionally, ATG6 and NPR1 synergistically promote the expression ofpathogenesis-relatedgenes. Further results showed that silencingATG6inNPR1-GFPexacerbatesPstDC3000/avrRps4invasion, while double overexpression ofATG6andNPR1synergistically inhibitsPstDC3000/avrRps4invasion. In summary, our findings unveil an interplay of NPR1 with ATG6 and elucidate important molecular mechanisms for enhancing plant immunity.HighlightWe unveil a novel relationship in which ATG6 positively regulates NPR1 in plant immunity.

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Expression of PEI-coated gold nanoparticles carrying exogenous gene in periwinkle mesophyll cells and its practice in huanglongbing research

Cited by 5 publications

References 43 publications

Chitosan-Modified Polyethyleneimine Nanoparticles for Enhancing the Carboxylation Reaction and Plants’ CO₂Uptake

Chitosan-Modified Polyethyleneimine Nanoparticles for Enhancing the Carboxylation Reaction and Plants’ CO₂Uptake

Three new discovery effector proteins from Candidatus Liberibacter asiaticus psy62 inhibit plant defense through interaction with AtCAT3 and AtGAPA

ATG6 interacting with NPR1 increasesArabidopsis thalianaresistance toPstDC3000/avrRps4by increasing its nuclear accumulation and stability

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Expression of PEI-coated gold nanoparticles carrying exogenous gene in periwinkle mesophyll cells and its practice in huanglongbing research

Cited by 5 publications

References 43 publications

Chitosan-Modified Polyethyleneimine Nanoparticles for Enhancing the Carboxylation Reaction and Plants’ CO2Uptake

Chitosan-Modified Polyethyleneimine Nanoparticles for Enhancing the Carboxylation Reaction and Plants’ CO2Uptake

Three new discovery effector proteins from Candidatus Liberibacter asiaticus psy62 inhibit plant defense through interaction with AtCAT3 and AtGAPA

ATG6 interacting with NPR1 increasesArabidopsis thalianaresistance toPstDC3000/avrRps4by increasing its nuclear accumulation and stability

Contact Info

Product

Resources

About

Chitosan-Modified Polyethyleneimine Nanoparticles for Enhancing the Carboxylation Reaction and Plants’ CO₂Uptake

Chitosan-Modified Polyethyleneimine Nanoparticles for Enhancing the Carboxylation Reaction and Plants’ CO₂Uptake