Expression of Pancreatic Endocrine Markers by Mesenchymal Stem Cells From Human Adipose Tissue

Abstract

Mesenchymal stem cells (MSCs) from human adipose tissue have a great potential for use in cell therapy due to their ease of isolation, expansion, and differentiation, besides the relative acceptance from the ethical point of view. Our intention was to isolate and promote in vitro expansion and differentiation of MSCs from human adipose tissue into cells with a pancreatic endocrine phenotype. Human adipose tissue obtained from patients undergoing abdominal dermolipectomy was digested with type I collagenase. MSCs isolated by plastic adherence and characterized by cytochemistry and FACS were expanded in vitro. MSC differentiation into an endocrine phenotype was induced over 2 to 4 months with high glucose (25 mmol/L) media containing nicotinamide, exendin-4, and 2-mercaptoethanol. Insulin and glucagon expressions were analyzed by immunofluorescence. Cells isolated from human adipose tissue and expanded in vitro expressed MSC markers as confirmed by FACS and cytochemistry. Insulin but not glucagon production by differentiated cells was demonstrated by immunofluorescence. MSCs isolated from human adipose tissue were induced to differentiate in vitro into an endocrine phenotype that expressed insulin.

Bioactive decellularized extracellular matrix-based hydrogel supports human adipose tissue-derived stem cell maintenance and fibrocartilage phenotype

“…The cells used in this study were harvested from adipose tissue derived from liposuction that would otherwise be discarded, therefore does not present ethical issues or risks for the donor, who provided written informed consent. Cells were obtained according to a previously described protocol ( Silva et al, 2012 ). Briefly, fragments of subcutaneous adipose tissue were washed with phosphate-buffered saline (PBS) and minced into small fragments before being digested with 1 mg/mL type I collagenase (Invitrogen ® , Grand Island, NY, United States).…”

Adipose tissue-derived stem cells show considerable promise for regenerative medicine applications

“…This is a promising direction of investigation toward more effective treatment of diabetes. Other researchers [30] also discovered that ADSCs differentiate into endocrine cells expressing insulin. ADSCs do that under the influence of high glucose levels in the base (25 mmol/l) and nicotinic acid (Nicotinamide), exendin-4, and 2-mercaptoethanol.…”