Expression of p63 immunostaining in liquid‐based cytology (BD SurePath) of breast fine‐needle aspiration

Osugi, Masumi; Kinoshita, Kosei; Sugita, Atsuro; Kito, Katsumi; Maeda, Toshiharu

doi:10.1002/dc.24041

Cited by 3 publications

(2 citation statements)

References 9 publications

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“…In these circumstances, IHC may have to be performed on cytology smear slides. Many studies have demonstrated that successful IHC staining can be achieved on a variety of cytology specimens including direct smears, 18–20 ThinPrep, 21,22 SurePath, 23,24 cytospin, 25 and slides prepared by cell transfer technique 26,27 . (Table 1) The only exception is Diff Quik stained slides, which are not suitable for IHC staining 26,27 …”

Section: Performing Ihc On Cytology Smears When An Adequate Cb Is Not...mentioning

confidence: 99%

“…All IHC need validation because of the use of methanol-based fixative, which may interfere IHC. Expensive equipment on a variety of cytology specimens including direct smears, [18][19][20] ThinPrep, 21,22 SurePath, 23,24 cytospin, 25 and slides prepared by cell transfer technique. 26,27 (Table 1) The only exception is Diff Quik stained slides, which are not suitable for IHC staining.…”

Section: Self-clottingmentioning

confidence: 99%

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Application of Immunohistochemistry in Cytology

Shi

Yee‐Chang

Shi

2022

Applied Immunohistochemistry &Amp; Molecular Morphology

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Immunohistochemistry (IHC), also referred to as immunocytochemistry in cytology literature, has revolutionized the practice of cytopathology. Because of the complexity of cytology preparation and limited diagnostic material, performing IHC remains a challenge. Formalin-fixed paraffin-embedded (FFPE) cell block (CB) is the optimal choice for IHC. In this review, the approaches for improving CB preparation will be discussed. When CB material is not available, various cytology specimens can also be used for IHC. With the utilization of Antigen Retrieval (AR) technique, these nonformalin-fixed cytology specimens can achieve successful IHC staining, comparable with the results from FFPE tissue sections. In the last part of this review, we will discuss the use of positive controls and the important role of AR in standardization of IHC in cytology.

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Section: Performing Ihc On Cytology Smears When An Adequate Cb Is Not...mentioning

confidence: 99%

Section: Self-clottingmentioning

confidence: 99%

Application of Immunohistochemistry in Cytology

Shi

Yee‐Chang

Shi

2022

Applied Immunohistochemistry &Amp; Molecular Morphology

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Genetic association scan of 32 osteoarthritis susceptibility genes identified TP63 associated with an endemic osteoarthritis, Kashin-Beck disease

Cheng¹,

Liang²,

Yang³

et al. 2021

Bone

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Advances in diagnostic liquid‐based cytology

Abe,

Kawahara,

Akiba

et al. 2024

Cytopathology

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Liquid‐based cytology (LBC) has changed the landscape of gynaecological cytology. A growing demand exists for LBC in diagnostic cytology, particularly for ancillary testing, such as immunocytochemistry and molecular testing. Ancillary testing solely based on conventional preparation (CP) methods remains challenging. Recently, the increased demand for specialist testing and minimally invasive techniques, such as endoscopic ultrasonography fine‐needle aspiration, to obtain cellular samples has led to an increasing demand for ancillary testing on cytology LBC supernatant, slides and cell block (CB). This facilitates the diagnosis and prognosis in cytology samples enabling personalized treatment. An understanding of the history and future prospects of LBC is crucial for its application in routine diagnostics by cytopathologists and cytotechnologists. In this review, we initiated an internet search using the keyword ‘liquid‐based cytology’, and we conducted a literature review to discuss the usefulness of combined diagnosis of LBC and CP, immunocytochemistry and molecular testing and assessed the quality of nucleic acids in diagnostic LBC. High‐quality and cell‐rich diagnostic LBC surpassed the CP method alone in terms of reliability and versatility of ancillary testing in cytological diagnosis. Conclusively, diagnostic LBC lends itself to various new technologies and is expected to continue evolving with innovations in the future.

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Expression of p63 immunostaining in liquid‐based cytology (BD SurePath) of breast fine‐needle aspiration

Abstract: The diagnostic accuracy of breast FNAC was significantly improved by adding LBC-p63 immunostaining.

Cited by 3 publications

References 9 publications

Application of Immunohistochemistry in Cytology

Application of Immunohistochemistry in Cytology

Genetic association scan of 32 osteoarthritis susceptibility genes identified TP63 associated with an endemic osteoarthritis, Kashin-Beck disease

Advances in diagnostic liquid‐based cytology

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