Expression of p53, <scp>K</scp>i67, <scp>EcPV</scp>2‐ and <scp>EcPV</scp>3 <scp>DNA</scp>, and viral genes in relation to metastasis and outcome in equine penile and preputial squamous cell carcinoma

Top, Janetta; Harkema, Liesbeth; Lange, Christian E.; Ensink, J. M.; Lest, Chris H.A. van de; Barneveld, A.; Weeren, P. R. van; Gröne, Andrea; Martens, Ann

doi:10.1111/evj.12245

Cited by 32 publications

(50 citation statements)

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“…4 In horses, oncogenic E6 transcript from EcPV2 was detected by reverse transcriptase PCR in genital SCCs, which suggests an active pathogenic role of the virus. 13,15 Nonetheless, because the virus replicates in surface epithelium, these techniques by themselves cannot demonstrate causality because they do not provide information on the distribution of expression within the tumor. Several studies have convincingly used oligomer in situ hybridization (ISH) to demonstrate that EcPV2 DNA is present within nuclei of a subset of tumor cells.…”

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confidence: 99%

Equine Genital Squamous Cell Carcinoma

et al. 2015

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Equus caballus papillomavirus 2 (EcPV2) has been proposed as an etiologic agent for genital squamous cell carcinoma (SCC), the most common malignant tumor of the horse penis. EcPV2 is commonly detected by polymerase chain reaction (PCR) on normal horse genitalia; therefore, unraveling the virus' role in oncogenic transformation requires other methods of detection. In this study, a highly sensitive multiple-probe chromogenic in situ hybridization (ISH) technique was designed to recognize the E6/E7 oncogenes of EcPV2. ISH demonstrated abundant virus within 6 of 13 penile and preputial SCCs, whereas evidence of solar damage was found in 6 cases that were negative for EcPV2 by ISH. The ISH technique is valuable for studies of pathogenesis, since it demonstrates for the first time that the vast majority of neoplastic cells contain virus. Moreover, hybridization was present in all metastases examined, implying stability of E6/E7 expression in these clonal populations of neoplastic cells. This study contributes to the accumulating evidence for a causal role of EcPV2 in a subset of genital SCCs in horses.

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confidence: 99%

Equine Genital Squamous Cell Carcinoma

et al. 2015

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“…Penile and preputial SCC mainly affects older horses and most studies report an average age of 17 to 20 years. 17,18 Carcinoma development has been attributed to the presence of bovine papilloma viruses but the relationship between the two conditions has not been definitively established. The virus can be found on normal sin and in particular on areas of inflamed or ulcerated skin in other conditions as well.…”

Section: Squamous Cell Carcinomamentioning

confidence: 99%

Integumentary Disorders Including Cutaneous Neoplasia in Older Horses

Knottenbelt

2016

Veterinary Clinics of North America: Equine Practice

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“…Expression of cell proliferation markers, such as Ki67 and tumour suppressor genes, for example p53, may also be evaluated using immunohistochemical staining of histopathological sections (Van den Top et al . ). Such markers are increasingly used in assessment of numerous types of small animal neoplasms such as canine mast cell tumours (Webster et al .…”

Section: Introductionmentioning

confidence: 97%

“…A positive correlation between high grade SCCs and metastasis has been demonstrated and it has also been shown that tumour grading is an important prognosticator for survival in horses (van den Top et al . , ). This information, in conjunction with a published classification system (Van den Top et al .…”

Section: Introductionmentioning

confidence: 99%