Expression of p53 Protein in Pterygium

Onur, C; Orhan, Dıclehan; Orhan, Mehmet; Sak, Serpil Dizbay; Tulunay, Özden; İrkeç, Murat

doi:10.1177/112067219800800307

Cited by 25 publications

(18 citation statements)

References 29 publications

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“…It is not yet clear how the tissue alterations are initiated and kept in progress. Favored but controversial [35,40] factors include the mutation of limbus stem cells [17,18,42], modification of stroma fibroblasts [3,5,6,7,22,27,29,30], and immunological and other processes in the matrix [3,12,23,37,45]. That the extracellular matrix can interact with the cells contacting it is an established biological fact [2,38].…”

Section: Discussionmentioning

confidence: 99%

Topological–histological investigation of the pterygium

Seifert

Eckert

Spitznas

2001

Graefe's Arch Clin Exp Ophthalmol

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Section: Discussionmentioning

confidence: 99%

Topological–histological investigation of the pterygium

Seifert

Eckert

Spitznas

2001

Graefe's Arch Clin Exp Ophthalmol

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“…The fact that different pools of p53 may exist in conjunctival and pterygia specimens explains the discrepant results seen by different groups with respect to p53 in that each group has employed different antibodies 45 28 …”

Section: Discussionmentioning

confidence: 99%

Apoptosis and apoptosis related gene expression in normal conjunctiva and pterygium

Tan

2000

British Journal of Ophthalmology

127

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Background-Pterygium is a relatively common eye disease in the tropics whose aetiology and pathogenesis remain uncertain. As such, interest has focused on understanding the underlying mechanism of pterygia development. Methods-15 specimens of pterygia from 15 eyes were examined, together with normal conjunctival tissue from the same eyes for the pattern of gene expression of genes associated with the induction or repression of apoptosis (p53, bcl-2, and bax). In addition, the samples directly for apoptotic cells were examined by the terminal deoxynucleotide transferase (TdT) mediated nick end labelling (TUNEL) methodology. Results-In pterygia specimens apoptotic cells were found mainly confined to the basal layer of cells of the epithelial layer, situated immediately adjacent to the fibrovascular support layer. These cells were shown to express significant levels of p53 and bax, as well as the apoptosis inhibiting protein bcl-2. In contrast, normal conjunctival specimens displayed no bcl-2 expression and apoptotic cells were seen throughout the entire width of the epithelial layer, coupled with high levels of bax expression. Conclusion-These results support a model whereby pterygia development is a result of disruption of the normal process of apoptosis occurring in the conjunctiva. (Br J Ophthalmol 2000;84:212-216)

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“…They proposed that this might indicate the existence of p53 mutation in the cells as an early event in their development, probably as a result of UV radiation exposure. Onur et al, 10 however, using immunohistochemical staining on 38 pterygia specimens, found that only 7.9% had a few p53 stained cells. In none of these studies was a control group used.…”

Section: Discussionmentioning

confidence: 99%

Overexpression of p53 tumor suppressor gene in pterygia

Weinstein

Rosenthal

Zirkin

et al. 2002

Eye

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Purpose To assess p53 gene expression in pterygia with and without recurrence. The pathogenesis of pterygium has not yet been determined. The most widely recognized etiologic factor is ultraviolet radiation, which leads to degeneration of the conjunctiva. However, pterygium was recently found to have several tumor-like characteristics. The p53 gene is a common marker for neoplasia, and is known to control cell cycle, cell differentiation and apoptosis. In this study we examined the expression of the p53 gene in primary pterygia with and without recurrence, searching for the pathogenesis of this very common lesion and for a prognostic factor for recurrence. Methods Immunohistochemical staining using a monoclonal antibody to human p53 (DO-7) was performed on 13 consecutive patients with primary pterygia, four pterygia without recurrence and nine pterygia which recurred during a 12-month follow-up. As a control we used two specimens of normal conjunctiva. Results Seven of the 13 pterygia specimens (54%) were positive for abnormal p53 expression. There was no difference between the groups with and without recurrence. Two out of four pterygia (50%) without recurrence and five out of nine (55.5%) pterygia with recurrence were positive. No pathological staining was observed in the control specimens. Conclusions In this study, abnormal p53 expression was found in pterygial epithelium, suggesting that pterygium could be a result of uncontrolled cell proliferation, and not as a degenerative lesion. There seems to be no connection between abnormal p53 expression and recurrence.

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Expression of p53 Protein in Pterygium

Abstract: We conclude that defective p53 tumor suppressor gene function seems to have no role in the pathogenesis of pterygium.

Cited by 25 publications

References 29 publications

Topological–histological investigation of the pterygium

Topological–histological investigation of the pterygium

Apoptosis and apoptosis related gene expression in normal conjunctiva and pterygium

Overexpression of p53 tumor suppressor gene in pterygia

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