Expression of p27 is associated with Bax expression and spontaneous apoptosis in oral and oropharyngeal carcinoma

Fujieda, Shigeharu; Inuzuka, Manabu; Tanaka, Nobuyuki; Sunaga, Hiroshi; Fan, Guo-Kang; Ito, Toshihisa; Sugimoto, Chizuru; Tsuzuki, Hideaki; Saitô, Hitoshi

doi:10.1002/(sici)1097-0215(19990621)84:3<315::aid-ijc20>3.3.co;2-l

Cited by 26 publications

(33 citation statements)

References 15 publications

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“…Several studies have analyzed c-myc expression in this type of tumor showing different results, with an average positivity of 41.28% (2.4-75%) between the different studies (6,(26)(27)(28)(29)(30)(31)(32)(33)(34). The results are extremely contradictory, as well as the variation in the quantification methods.…”

Section: Discussionmentioning

confidence: 99%

Quantitative determination of c-myc facilitates the assessment of prognosis of OSCC patients

et al. 2014

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Abstract.Myc genes are a family of proto-oncogenes whose proteins are implicated in the regulation of cell proliferation, differentiation and apoptosis, and in regulating the activity of genes involved in cell division. The aim of the present study was to establish a quantitative description of the expression of c-myc and evaluate its relationship with other clinical and prognostic factors, as well as to establish a multivariate survival prediction model. This is a retrospective study of 68 patients diagnosed with oral squamous cell carcinoma (OSCC). We constructed a tissue microarray for investigating the expression of c-myc by immunohistochemistry. Statistical analyses were carried out, and a multivariate model that predicts survival was established. The average expression of c-myc was 50.32 (SD, 26.05) with a range from 6.60 to 99.

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Section: Discussionmentioning

confidence: 99%

Quantitative determination of c-myc facilitates the assessment of prognosis of OSCC patients

et al. 2014

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“…This gene regulates G 0 -to-S phase transitions by interacting with cyclindependent kinases (CDKs) (Hengst and Reed 1998;Sherr and Roberts 1999), and overexpression of this gene has been shown to trigger apoptosis in the MDA-MB-231 cell line (Katayose et al 1997) with pro-apoptotic properties in other cancers (Fujieda et al 1999;Wu et al 1999;Tenjo et al 2000). Apoptosis has recently been identified as one of the deregulated pathways by the miR-182-5p cluster in medulloblastoma (Weeraratne et al 2012), and suppression of CDKN1B by miR-182-5p, along with other pro-apoptotic genes like BAK and BAX (Supplemental Table 1), suggests that apoptosis triggered by DNA damage could be deregulated by this miRNA.…”

Section: Kip1mentioning

confidence: 99%

MicroRNA-182-5p targets a network of genes involved in DNA repair

Krishnan

Steptoe

Martin

et al. 2012

RNA

113

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MicroRNAs are noncoding regulators of gene expression, which act by repressing protein translation and/or degrading mRNA. Many have been shown to drive tumorigenesis in cancer, but functional studies to understand their mode of action are typically limited to single-target genes. In this study, we use synthetic biotinylated miRNA to pull down endogenous targets of miR-182-5p. We identified more than 1000 genes as potential targets of miR-182-5p, most of which have a known function in pathways underlying tumor biology. Specifically, functional enrichment analysis identified components of both the DNA damage response pathway and cell cycle to be highly represented in this target cohort. Experimental validation confirmed that miR-182-5p-mediated disruption of the homologous recombination (HR) pathway is a consequence of its ability to target multiple components in that pathway. Although there is a strong enrichment for the cell cycle ontology, we do not see primary proliferative defects as a consequence of miR-182-5p overexpression. We highlight targets that could be responsible for miR-182-5p-mediated disruption of other biological processes attributed in the literature so far. Finally, we show that miR-182-5p is highly expressed in a panel of human breast cancer samples, highlighting its role as a potential oncomir in breast cancer.

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“…Immunohistochemical staining was performed to detect Syk in human nasal fibroblasts using the traditional avidin-biotin-peroxidase complex technique (29). After purified fibroblasts were seeded and cultured on chamber slide-glasses, the cells were fixed with 70% ethanol.…”

Section: Immunohistochemical Stainingmentioning

confidence: 99%

Protein-Tyrosine Kinase Syk Expressed in Human Nasal Fibroblasts and Its Effect on RANTES Production

Yamada

Fujieda

Yanagi

et al. 2001

The Journal of Immunology

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Fibroblasts, a rich source of chemokines, interact with eosinophils and play a key role in the pathogenesis of airway disease. RANTES is produced by fibroblasts to attract and activate eosinophils. LPS is known to induce RANTES and cause protein tyrosine phosphorylation. Nonreceptor protein tyrosine kinase Syk is widely expressed and an important role in intracellular signal transduction in hemopoietic cells. In the present study, we examined whether Syk was expressed in a number of primary human nasal polyp tissue-derived fibroblast lines and whether it played some role in cellular function. Syk proteins were expressed in human nasal fibroblasts, but the expression level varied. There were positive correlations between the level of Syk expression and RANTES production induced by LPS. Overexpression of wild-type Syk by gene transfer enhanced RANTES production from nasal fibroblasts stimulated with LPS. The decrease of Syk expression by the administration of Syk antisense inhibited RANTES production. These results suggest that Syk expression affects RANTES production in fibroblasts of nasal polyps.

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Expression of p27 is associated with Bax expression and spontaneous apoptosis in oral and oropharyngeal carcinoma

Cited by 26 publications

References 15 publications

Quantitative determination of c-myc facilitates the assessment of prognosis of OSCC patients

Quantitative determination of c-myc facilitates the assessment of prognosis of OSCC patients

MicroRNA-182-5p targets a network of genes involved in DNA repair

Protein-Tyrosine Kinase Syk Expressed in Human Nasal Fibroblasts and Its Effect on RANTES Production

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