Expression of P-selectin (CD62P) on platelets after thrombin and ADP in hypotrophic and healthy, full-term newborns

Wasiluk, Alicja; Kemona, H; Mantur, M; Polewko, Agnieszka; Ozimirski, Andrzej; Milewski, Robert

doi:10.3109/14767058.2013.784251

Cited by 7 publications

(4 citation statements)

References 23 publications

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“…Wasiluk et al 55 reported that the thrombopoietin level in SGA newborns is higher than that in healthy term newborns and is positively correlated with the severity of the SGA, although no association could be found in the SGA group between platelet count and TPO. In another study by Wasiluk et al, 56 4.21% of the platelets of IUGR neonates expressed P-selectin (CD62P), a marker of platelet stimulation, versus 2.88% of the AGA control group. After the addition of platelet activators such as thrombin and ADP, there was a statistically significant increase in platelet activation in IUGR infants compared with AGA.…”

Section: Resultsmentioning

confidence: 95%

“…80 In addition, the incidence of DIC and neonatal thrombosis and stroke is increased in FGR neonates. 7 16 18 20 21 27 Besides incidences of neonatal DIC and thrombosis, which are known causes of increased platelet consumption and destruction, intraplacental platelet consumption and thrombi formation is presumed as another possible mechanism for thrombocytopenia, as supported by Wasiluk et al 55 56 In pregnancies complicated by FGR placental insufficiency is caused by anomalous villous vessels, endothelial dysfunction, leading to placental vasoconstriction, platelet activation, and high UA-PI. As a result, a microangiopathic condition is triggered, causing platelet consumption and eventually a small placenta.…”

Section: Discussionmentioning

confidence: 99%

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Assessment of Hemostatic Profile in Neonates with Intrauterine Growth Restriction: A Systematic Review of Literature

Karapati

Sokou

Iliodromiti

et al. 2023

Semin Thromb Hemost

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Intrauterine growth restriction (IUGR) affects nearly 10 to 15% of pregnancies and is responsible for many short- and long-term adverse consequences, including hemostatic derangement. Both thrombotic and hemorrhagic events are described in the perinatal period in these neonates. The aim of this study was to systematically review the literature on the laboratory studies used to evaluate the hemostatic system of the IUGR small for gestational age neonate. We reviewed the current literature via PubMed and Scopus until September 2022. Following our inclusion/exclusion criteria, we finally included 60 studies in our review. Thrombocytopenia, characterized as hyporegenerative and a kinetic upshot of reduced platelet production due to in utero chronic hypoxia, was the main finding of most studies focusing on growth-restricted neonates, in most cases is mild and usually resolves spontaneously with the first 2 weeks of life. In regard to coagulation, growth-restricted newborns present with prolonged standard coagulation tests. Data regarding coagulation factors, fibrinolytic system, and anticoagulant proteins are scarce and conflicting, mainly due to confounding factors. As thromboelastography/rotational thromboelastometry (TEG/ROTEM) provides a more precise evaluation of the in vivo coagulation process compared with standard coagulation tests, its use in transfusion guidance is fundamental. Only one study regarding TEG/ROTEM was retrieved from this population, where no difference in ROTEM parameters compared with appropriate for gestational age neonates was found. Despite the laboratory aberrations, no correlation could be achieved with clinical manifestations of bleeding or thrombosis in the studies included. More studies are needed to assess hemostasis in IUGR neonates and guide targeted therapeutic interventions.

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Section: Resultsmentioning

confidence: 95%

Section: Discussionmentioning

confidence: 99%

Assessment of Hemostatic Profile in Neonates with Intrauterine Growth Restriction: A Systematic Review of Literature

Karapati

Sokou

Iliodromiti

et al. 2023

Semin Thromb Hemost

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“…The hemostatic profile of IUGR neonates is an area of wide interest, as they experience hemorrhagic, as well as thrombotic complications during the neonatal period, such as pulmonary and gastrointestinal hemorrhage, hemorrhagic neonatal stroke, and disseminated intravascular coagulopathy (DIC) [7][8][9][10][11][12][13][14]. In IUGR pregnancies, placental insufficiency is the result of thrombi formation, defective endothelium, intraplacental platelet activation, and consumption, leading to small placentas and neonatal thrombocytopenia [15][16][17].…”

Section: Introductionmentioning

confidence: 99%

Hemostatic Profile of Intrauterine Growth-Restricted Neonates: Assessment with the Use of NATEM Assay in Cord Blood Samples

Karapati,

Valsami,

Sokou

et al. 2024

Diagnostics

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Background: Intrauterine growth restriction (IUGR) is associated with hemorrhagic and thrombotic complications during the perinatal period. Thrombocytopenia, platelet dysfunction, and prolonged standard coagulation tests are observed in this population. The aim of this study is to examine the hemostatic profile of IUGR neonates with the use of a non-activated assay (NATEM) in cord blood samples. Methods: During an 18 month period, a NATEM ROTEM assay was performed on cord blood samples of 101 IUGR neonates. A total of 189 appropriate for gestational age (AGA) neonates were used as a control group. The NATEM variables recorded include the following: clotting time (CT); clot formation time (CFT); clot amplitude at 5, 10, and 20 min (A5, A10, A20); α-angle (a°); maximum clot firmness (MCF); lysis index at 30 and 60 min (LI30, LI60); and maximum clot elasticity (MCE). Results: IUGR neonates demonstrate a hypocoagulable state, with lower A5, A10, A2, MCF, and MCE values when compared to AGA. Using multiple linear regression, we determined IUGR as an independent factor influencing all NATEM parameters (except CT and LI30) exhibiting a hypocoagulable and hypofibrinolytic profile. Platelet count was positively correlated with A5, A10, A20, MCF, alpha angle, and MCE, and negatively correlated with CFT. Conclusion: IUGR neonates appear with lower clot strength and elasticity and prolonged clot kinetics, as illustrated by ROTEM variables.

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“…However, whether the process could result in platelet activation damage is a question that has caused widespread concern and remains unanswered. Laboratory indicators for evaluating the activation of platelets include β-TG, GPIb (CD42), the platelet aggregation test or GPIIb/IIIa (CD41), soluble P-selectin, platelet surface P-selectin (CD62) (Wasiluk et al, 2013), among which CD62 is the best. Thus, we detected and analyzed the platelet P-selectin (CD62P) surface expression of apheresis platelets with different retention periods using flow cytometry before and after leukocyte filtration to evaluate the effects of leukocyte filtration on platelet activation.…”

Section: Introductionmentioning

confidence: 99%

Effect of leukocyte filtration on the P-selectin expression of apheresis platelets

Chen

Zhang

et al. 2015

Genet. Mol. Res.

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ABSTRACT. The aim of this study was to investigate the effect of leukocyte filtration on the P-selectin (CD62P) surface expression of apheresis platelets during the retention period. Ten bags of apheresis platelets stored for 1 day (0-24 h) and 10 bags of apheresis platelets stored for 2 days (24-48 h) were used for leukocyte filtration (experimental group). Ten bags of apheresis platelets with the corresponding retention periods but without filtration were used as a negative control (control group). Thereafter, 100 μL of platelet suspensions from apheresis platelets with or without leukocyte filtration were sampled before and after leukocyte filtration for the detection of CD62P surface expression by flow cytometry. No statistical difference in the CD62P (2015) surface expression of apheresis platelets was observed before and after leukocyte filtration (P > 0.05), neither did the CD62P surface expression exhibit any change among the different retention periods. Leukocyte filtration does not affect the CD62P surface expression of apheresis platelets stored for up to 2 days, which indicates that leukocyte filtration does not damage the activation of apheresis platelets within the retention period.

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Expression of P-selectin (CD62P) on platelets after thrombin and ADP in hypotrophic and healthy, full-term newborns

Abstract: Hypotrophic newborns are capable of greater platelet activation in comparison with healthy term newborns. However, gender does not affect the expression of P-selectin.

Cited by 7 publications

References 23 publications

Assessment of Hemostatic Profile in Neonates with Intrauterine Growth Restriction: A Systematic Review of Literature

Assessment of Hemostatic Profile in Neonates with Intrauterine Growth Restriction: A Systematic Review of Literature

Hemostatic Profile of Intrauterine Growth-Restricted Neonates: Assessment with the Use of NATEM Assay in Cord Blood Samples

Effect of leukocyte filtration on the P-selectin expression of apheresis platelets

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