Expression of P-glycoprotein in L1210 cells is linked with rise in sensitivity to Ca2+

Sulová, Zdena; Orlický, J; Fiala, Roderik; Dovinová, Ima; Uhrík, B; Šereš, Mário; Gibalová, Lenka; Breier, Albert

doi:10.1016/j.bbrc.2005.07.144

Cited by 18 publications

(15 citation statements)

References 24 publications

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“…The fact that TNM is able to block protein glycosylation, including P-gp itself, in P-gp-positive cells (Morin and Bernacki 1983;Loo and Clarke 1993;Seres et al 2011) is contradictory to this speculation. Moreover, resistance of L1210/R and L1210/T cells to TNM could not be reversed by verapamil (a known P-gp inhibitor) even if this substance completely blocked P-gp efflux activity measured by calcein retention assay (Orlicky et al 2004;Sulova et al 2005). TNM enhances the sensitivity of not only drug-resistant NIH-3T3-MDR cells but also drugsensitive NIH-3T3 cells to P-gp substrates (such as DOX and VCR) or to cisPt, a P-gp-untransportable drug (Hiss et al 1996).…”

Section: Discussionmentioning

confidence: 99%

The expression of P-gp in leukemia cells is associated with cross-resistance to protein N-glycosylation inhibitor tunicamycin

Pavlíková

Šereš²,

Imrichova³

et al. 2016

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Abstract. In P-gp-positive cell variants obtained from L1210 cells either by selection with vincristine (L1210/R) or by transfection with the human gene encoding P-gp (L1210/T), we have previously described cross-resistance to tunicamycin (TNM), a protein N-glycosylation inhibitor. Here we studied whether this cross-resistance also underlies P-gp-positive variants of human acute myeloid leukemia cells (AML) derived from SKM-1 and MOLM-13 cells (SKM-1/VCR, SKM-1/LEN, MOLM-13/ VCR) by selection with vincristine (VCR) and lenalidomide (LEN). While SKM-1/LEN cells were P-gp positive, no P-gp was detected in MOLM-13/LEN cells. P-gp-positive cells could be repeatedly passaged in medium containing TNM. In contrast, more than 90% of P-gp-negative cells were entering and progressing through cell death mechanisms after the third passage in medium containing TNM. Combined apoptosis/necrosis cell death was detected in L1210 cells after exposure to TNM. Passaging of P-gp-negative AML cells in medium containing TNM induced preferentially apoptosis. Damage to P-gp-negative cells induced with TNM was associated with arrest in the G1 phase of the cell cycle. P-gp-positive leukemia cells differed from P-gp-negative cells in the composition of plasma membrane glycoproteins, which we monitored with the aid of different lectins. The application of TNM to cells induced additional changes in membrane-linked glycosides.

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Section: Discussionmentioning

confidence: 99%

The expression of P-gp in leukemia cells is associated with cross-resistance to protein N-glycosylation inhibitor tunicamycin

Pavlíková

Šereš²,

Imrichova³

et al. 2016

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“…Dose-response curves were fitted according to an exponential decay equation (Eq. 1) by non-linear regression as previously described (Kupsakova et al 2004;Sulova et al 2005):…”

Section: Cells (5 × 10mentioning

confidence: 99%

Vincristine-induced expression of P-glycoprotein in MOLM-13 and SKM-1 acute myeloid leukemia cell lines is associated with coexpression of nestin transcript

Imrichova¹,

Coculova²,

Messingerová³

et al. 2014

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Abstract.Nestin is a class 6 filament protein typically expressed in neural stem/progenitor cells. However, nestin expression has been observed in other tissues during mammalian embryogenesis. In human neural stem/progenitor cells, coexpression of nestin and P-glycoprotein (P-gp, ABCB1 member of the ABC transporter family) was detected. P-gp-mediated drug efflux is the most common molecular cause of multidrug resistance in neoplastic cells. Nestin expression has also been detected in various human solid tumours as well as in the corresponding established cell lines. Interestingly, expression of nestin in different leukemia cells has been recently reported. Here, we show that expression of P-gp is associated with the simultaneous expression of nestin in acute myeloid leukemia cell lines (MOLM-13 and SKM-1) under the selective pressure of vincristine, a substance that may induce P-gp expression in neoplastic cells.

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“…38 Therefore, Ca 2þ signaling is an essential component of the mitogen responses and studies have revealed that the sensitivity to Ca 2þ of multidrug-resistant cells differs from that of drug-sensitive cells. 22 In this study, MCF-7/ADR cells were exposed to 2 mM doxorubicin in the presence of EBB for 24 h, and EBB strongly synergized the doxorubicin-induced G 2 /M arrest during cell-cycle progression in the MCF7/ADR cells (In Fig. 7).…”

Section: Discussionmentioning

confidence: 99%

“…21 Moreover, the sensitivity of multidrug-resistant cells to Ca 2þ differs from that of drug-sensitive cells. 22 We reported that a soluble resistance-related calcium-binding protein was upregulated in cells transformed with the mdr1gene. 23 Therefore, Ca 2þ /CaM is recognized as a potential target for cancer chemotherapy 24 and several calmodulin inhibitors have been shown to reverse MDR by inhibiting P-glycoprotein-mediated drug efflux and interfering with cell-cycle progression.…”

Section: Introductionmentioning

confidence: 99%

A Novel Calmodulin Antagonist O-(4-Ethoxyl-Butyl)-Berbamine Overcomes Multidrug Resistance in Drug-Resistant MCF-7/ADR Breast Carcinoma Cells

Liu

Zhang

Chen

et al. 2010

Journal of Pharmaceutical Sciences

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Expression of P-glycoprotein in L1210 cells is linked with rise in sensitivity to Ca2+

Cited by 18 publications

References 24 publications

The expression of P-gp in leukemia cells is associated with cross-resistance to protein N-glycosylation inhibitor tunicamycin

The expression of P-gp in leukemia cells is associated with cross-resistance to protein N-glycosylation inhibitor tunicamycin

Vincristine-induced expression of P-glycoprotein in MOLM-13 and SKM-1 acute myeloid leukemia cell lines is associated with coexpression of nestin transcript

A Novel Calmodulin Antagonist O-(4-Ethoxyl-Butyl)-Berbamine Overcomes Multidrug Resistance in Drug-Resistant MCF-7/ADR Breast Carcinoma Cells

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Expression of P-glycoprotein in L1210 cells is linked with rise in sensitivity to Ca2+

Cited by 18 publications

References 24 publications

The expression of P-gp in leukemia cells is associated with cross-resistance to protein N-glycosylation inhibitor tunicamycin

The expression of P-gp in leukemia cells is associated with cross-resistance to protein N-glycosylation inhibitor tunicamycin

Vincristine-induced expression of P-glycoprotein in MOLM-13 and SKM-1 acute myeloid leukemia cell lines is associated with coexpression of nestin transcript

A Novel Calmodulin Antagonist O-(4-Ethoxyl-Butyl)-Berbamine Overcomes Multidrug Resistance in Drug-Resistant MCF-7/ADR Breast Carcinoma Cells

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Vincristine-induced expression of P-glycoprotein in MOLM-13 and SKM-1 acute myeloid leukemia cell lines is associated with coexpression of nestin transcript