Expression of oxygen-regulated protein 150 (ORP150) in skin wound healing and its application for wound age determination

Abstract

Immunohistochemical study combined with morphometry was carried out to examine the expression of oxygen-regulated protein 150 (ORP150) using 58 human skin wounds of different ages (group I, 0-12 h; II, 1-5 days; III, 7-14 days; and IV, 17-21 days). In human wound specimens aged 4-12 h, neutrophils recruited at the wound showed no positive signals for ORP150. With the increase in wound age of > or =7 days, granulation tissue and angiogenesis were observed, with the migration of macrophages and fibroblasts with ORP150-positive reactions. In semi-quantitative analysis, the average of ORP150-positive ratios in group III was highest. In group III, all samples had an ORP150-positive ratio of >40%, and 17 samples showed >50%. In group IV, three out of ten samples showed a positive ratio of 40-45% and the remaining seven cases less than 40%. Collectively, with regard to the practical applicability with forensic safety, these observations suggest that an ORP150-positive ratio of >50% strongly indicates a wound age of 7-14 days.

Time-dependent appearance of intrathrombus neutrophils and macrophages in a stasis-induced deep vein thrombosis model and its application to thrombus age determination

“…In forensic practice, it is most important to determine the post-infliction intervals of skin wounds or brain injuries, because forensic pathologists are required to judge how wounds or injuries are related to the cause of death [3][4][5][6][7][8][9][10][11][12]. Similarly, in cases of pulmonary thromboembolism, forensic pathologists have to determine the age of a thrombus in order to judge the causal relationship between the venous thrombus and trauma.…”

Detection of fibrocytes in human skin wounds and its application for wound age determination

“…The molecular mechanisms of wound repair are not fully understood but involve growth factors, cytokines, adhesion molecules, and matrix metalloproteases [9][10][11][12][13]. Some of these substances have been used for the determination of wound age and wound vitality [14][15][16][17][18][19][20][21][22][23][24][25][26][27][28].…”

Angiogenesis in brain contusion

“…W cytowanym badaniu ekspresja tego białka była istotnie niższa w porówna-niu z grupą kontrolną i grupą przypadków zgonów w przebiegu wstrząsu hipowolemicznego. Kolejnym kierunkiem badawczym, analizowanym zarówno w modelach zwierzęcych [8], jak i na materiale ludzkim [9,10], jest ocena wieku ran na podstawie immuprocess of vasculogenesis which mainly occurs during embryonic development [2,3].…”

“…In the cited study, the expression of the protein was significantly lower in relation to the control group and the group of deaths from hypovolaemic shock. Another research direction, which is pursued both in animal models [8] and the human material [9,10], is the assessment of wound age on the basis of the immunohistochemical expression of factors involved in the formation of new blood nohistochemicznej ekspresji czynników związanych z tworzeniem nowych naczyń krwionośnych i limfatycznych oraz hipoksją tkankową, takich jak CD31, czynnik von Willebranda (vWF), VEGF, podoplanina, ORP150 i izoformy syntazy tlenku azotu (NOS) [8][9][10][11]. Na uwagę zasługują także próby wykorzystania oceny pourazowej ekspresji cyklooksygenaz COX-1 i COX-2 w mózgu [12].…”

“…: angiostatyna, endostatyna, trombospondyna 1, czynnik płyt-kowy 4 (PF 4) i interferon α (IFN-α). and lymphatic vessels, and tissue hypoxia -including CD31, von Willebrand factor (vWF), VEGF, podoplanin, ORP150 and NOS (nitric oxide synthase) isoforms (NOS) [8][9][10][11]. Also noteworthy are the attempts to use the assessment of post-traumatic expression of cyclooxygenases COX-1 and COX-2 in the brain [12].…”