Expression of Osterix and SOX9 after Administration of Gourami Fish Scale Collagen in Wistar Rats

Abstract

The current goal of periodontal treatments is to regenerate periodontal tissue by adding bone grafts. Various materials are being explored as bone substitutes, including type 1 collagen. Gourami fish scales are known to be an alternative source of type 1 collagen and have potential as a substitute material for bone grafts. The objective of this study was to determine whether there is an increase in the expression of Osterix (OSX) and SOX9 after the administration of gourami (Osphronemus goramy) fish scale collagen in extracted socket Wistar rats (Rattus norvegicus). The research was conducted on 32 male Wistar rats that were classified into four groups: control groups on day 7 (K7) and day 14 (K14); and treatment groups on day 7 (P7) and day 14 (P14). The expression of OSX and SOX9 was visualized by immunohistochemical staining of bone tissue preparations by anti-OSX and SOX9 monoclonal antibodies. The ANOVA results for OSX expression had a significant difference in OSX expression between that in the control and treatment groups on day 7 and day 14. The ANOVA results for SOX9 expression on day 7 had a significant difference in SOX9 expression between that in the control and treatment groups. ANOVA results for SOX9 expression on day 14 had no significant difference in the expression of SOX9 between the treatment and control groups. The conclusion of this study was administration of Gourami scale collagen can increase the expression of OSX and SOX9 in socket’s Wistar rats. Keywords: Bone grafts, Type 1 collagen, Gourami fish scales, Osterix, SOX9