Expression of osteopontin in gentamicin-induced acute tubular necrosis and its recovery process

Xie, Yuansheng; Nishi, Shinichi; Iguchi, Seitaro; Imai, Naofumi; Sakatsume, Minoru; Saito, Akihiko; Ikegame, Mika; Iino, Noriaki; Shimada, Hideaki; Ueno, Mitsuhiro; Kawashima, Hiroyuki; Arakawa, Masaaki; Gejyo, Fumitake

doi:10.1046/j.1523-1755.2001.00579.x

Cited by 25 publications

(26 citation statements)

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“…In situ hybridization with rat MMP-12 mRNA was conducted as previously described (21). For digoxigenin (Dig)-labeled probe synthesis, in vitro transcription was performed using a Dig RNA labeling kit (SP6/T7; Roche, Mannheim, Germany).…”

Section: In Situ Hybridizationmentioning

confidence: 99%

Macrophage Metalloelastase as a Major Factor for Glomerular Injury in Anti-Glomerular Basement Membrane Nephritis

Kaneko

Sakatsume

Xie

et al. 2003

The Journal of Immunology

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Rat anti-glomerular basement membrane (GBM) nephritis is a model of crescentic glomerulonephritis induced by injection of anti-GBM antiserum. To elucidate the mechanism of glomerular injury, we analyzed the gene expression patterns in the kidneys of anti-GBM nephritis rats using DNA arrays, and found that macrophage metalloelastase/matrix metalloproteinase (MMP)-12 was one of the highly expressed genes in the kidneys on days 3 and 7 after the injection of anti-GBM antiserum. Enhancement of MMP-12 mRNA expression was confirmed by Northern blot analysis, and in situ hybridization revealed that MMP-12 mRNA was expressed in ED-1-positive macrophages and multinuclear giant cells in the glomeruli with crescent. Moreover, these cells were positive with anti-rat rMMP-12 Ab on the section of the kidneys of anti-GBM nephritis rats on day 7. To clarify the role of MMP-12, we conducted a neutralization experiment using anti-rat rMMP-12 Ab, which had an ability to inhibit rMMP-12 activity of degrading natural substrate such as bovine elastin or human fibronectin in vitro. Anti-rat rMMP-12 Ab or control Ig was injected in each of six rats on days 0, 2, 4, and 6 after the injection of anti-GBM antiserum. Consequently, crescent formation and macrophage infiltration in the glomeruli were significantly reduced in the rats treated with anti-rat rMMP-12 Ab, and the amount of urine protein was also decreased. These results disclosed that MMP-12 played an important role in glomerular injury in a crescentic glomerulonephritis model, and inhibition of MMP-12 may lead to a new therapeutic strategy for this disease.

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Section: In Situ Hybridizationmentioning

confidence: 99%

Macrophage Metalloelastase as a Major Factor for Glomerular Injury in Anti-Glomerular Basement Membrane Nephritis

Kaneko

Sakatsume

Xie

et al. 2003

The Journal of Immunology

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“…Several reports have shown that SPP1 increases tumor cell angiogenesis in mice (Takahashi et al 2002, Hirama et al 2003 and enhances vascularization in ectopic bone (Asou et al 2001). Xie et al (2001) demonstrated that an increase in SPP1 is associated with proliferation and regeneration of renal tubular epithelial cells following renal toxic or ischemic injury. Silencing of SPP1 markedly decreased the cell motility and invasiveness of SK-Hep-1 tumor cells (Cheng et al 2007) and metastasis of murine colon adenocarcinoma (Wai et al 2005).…”

Section: Introductionmentioning

confidence: 99%

Expression and regulation of secreted phosphoprotein 1 in the bovine corpus luteum and effects on T lymphocyte chemotaxis

Poole¹,

Ndiaye²,

Pate³

2013

Reproduction

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Secreted phosphoprotein 1 (SPP1) in the bovine corpus luteum (CL) regulates cell function during the transitional periods of luteinization and luteal regression. The objectives were to i) characterize SPP1 expression in the CL throughout the estrous cycle, ii) determine factors that regulate SPP1 expression in luteal cells, and iii) examine the role of SPP1 in lymphocyte chemotaxis, proliferation, and function. SPP1 mRNA was greater in fully functional (d10) CL and late cycle (d18) CL compared with developing (d4) CL. Additionally, SPP1 mRNA increased within 1 h and remained elevated 4 and 8 h following induction of luteolysis with prostaglandin (PG)F 2a . Expression of the SPP1 receptor, b 3 integrin, was not different throughout the estrous cycle but decreased following induction of luteolysis. Expression of CD44 increased during the estrous cycle but did not change during luteal regression. In cultured luteal cells, SPP1 mRNA was upregulated by PGF 2a and/or tumor necrosis factor a. Western blots revealed the presence of both full-length SPP1 and multiple cleavage products in cultured luteal cells and luteal tissue. Depletion of endogenous SPP1 did not hinder luteal cell-induced lymphocyte proliferation or lymphocyte phenotype but did inhibit lymphocyte migration toward luteal cells. Based on these data, it is concluded that SPP1 is initially activated to establish and maintain cellular interactions between steroidogenic and nonsteroidogenic cells during the development of the CL. Upon induction of luteolysis, SPP1 serves as a signaling molecule to recruit or activate immune cells to facilitate luteal regression and tissue degradation.

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“…In mammals, including humans, ototoxicity is a serious, and mostly permanent, side-effect of aminoglycoside therapy, whereas nephrotoxicity is often acute and reversible. Kidney tubule epithelia retain their ability to undergo cellular proliferation following aminoglycoside toxicity, unlike sensory epithelia in the mammalian inner ear (Chen and Segil, 1999;Nonclercq et al, 1992;Xie et al, 2001). …”

Section: Introductionmentioning

confidence: 99%

Uptake of fluorescent gentamicin by vertebrate sensory cells in vivo

et al. 2006

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Aminoglycoside uptake in the inner ear remains poorly understood. We subcutaneously injected a fluorescently-conjugated aminoglycoside, gentamicin-Texas Red (GTTR), to investigate the in vivo uptake of GTTR in the inner ear of several vertebrates, and in various murine sensory cells using confocal microscopy.In bullfrogs, GTTR uptake was prominent in mature hair cells, but not in immature hair cells. Avian hair cells accrued GTTR more rapidly at the base of the basilar papilla. GTTR was associated with the hair bundle; and, in guinea pigs and mice, somatic GTTR fluorescence was initially diffuse before punctate (endosomal) fluorescence could be observed. A baso-apical gradient of intracellular GTTR uptake in guinea pig cochleae could only be detected at early time points (<3 h). In 21−28 day mice, cochlear GTTR uptake was greatly reduced compared to guinea pigs, 6-day-old mice, or mice treated with ethacrynic acid. In mice, GTTR was also rapidly taken up, and retained, in the kidney, dorsal root and trigeminal ganglia. In linguinal and vibrissal tissues rapid GTTR uptake cleared over a period of several days.The preferential uptake of GTTR by mature saccular, and proximal hair cells resembles the pattern of aminoglycoside-induced hair cell death in bullfrogs and chicks. Differences in the degree of GTTR uptake in hair cells of different species suggests variation in serum levels, clearance rates from serum, and/or the developmental and functional integrity of the blood-labyrinth barrier. GTTR uptake by hair cells in vivo suggests that GTTR has potential to elucidate aminoglycoside transport mechanisms into the inner ear, and as a bio-tracer for in vivo pharmacokinetic studies.

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Expression of osteopontin in gentamicin-induced acute tubular necrosis and its recovery process

Cited by 25 publications

References 0 publications

Macrophage Metalloelastase as a Major Factor for Glomerular Injury in Anti-Glomerular Basement Membrane Nephritis

Macrophage Metalloelastase as a Major Factor for Glomerular Injury in Anti-Glomerular Basement Membrane Nephritis

Expression and regulation of secreted phosphoprotein 1 in the bovine corpus luteum and effects on T lymphocyte chemotaxis

Uptake of fluorescent gentamicin by vertebrate sensory cells in vivo

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