Expression of orexins and their precursor in the porcine ovary and the influence of orexins on ovarian steroidogenesis in pigs

Nitkiewicz, Anna; Smolińska, Nina; Maleszka, Anna; Chojnowska, Katarzyna; Kamiński, Tadeusz

doi:10.1016/j.anireprosci.2014.03.015

Cited by 28 publications

(37 citation statements)

References 39 publications

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“…Regarding the presence of PPO in the gonads of different species, various results were obtained. The presence of PPO mRNA was not demonstrated in the rat ovary (Silveyra et al 2007) while it was showed in the porcine ovary (J€ ohren et al 2001;Nitkiewicz et al 2014). These conflicting results may suggest that the localization of the PPO in the ovary is species dependent.…”

Section: Discussionmentioning

confidence: 87%

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Orexin A Expression in the Ovary of Dog and Cat

Levanti

Germanà

Abbate

2015

Reprod Domestic Animals

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Orexin A and B, also known as hypocretin A and B, are hypothalamic neuropeptides arising from a precursor to the 130 amino acid, called pre-pro orexin. They are synthesized mainly in lateral and posterior hypothalamus and are involved in different functions such as regulation of food intake and energy balance. Orexins and orexin receptors were previously described also in different tissues and organs outside the brain. The aim of this study was to demonstrate by means of the immunofluorescence technique, the presence of orexin A in the ovary of cat and dog, to support the hypothesis of the role of this substance also at the level of the female genital system. The presence of orexin A in the ovary either in dog or in cat is in agreement with previous data on the presence and role of orexins in the female genital system of other species.

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Section: Discussionmentioning

confidence: 87%

“…2007Nitkiewicz et al 2010). In the porcine ovary, the OX1 and OX2 localization at the level of mRNA and protein varies throughout the oestrous cycle (Nitkiewicz et al 2010(Nitkiewicz et al , 2014. Regarding the presence of PPO in the gonads of different species, various results were obtained.…”

Section: Discussionmentioning

confidence: 99%

Orexin A Expression in the Ovary of Dog and Cat

Levanti

Germanà

Abbate

2015

Reprod Domestic Animals

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“…The involvement of orexins in the control of endometrial epithelial cell proliferation through type 2 receptors has been suggested in only one study (Dehan et al, 2013). Our previous results suggest that orexins influence ovarian steroidogenesis in pigs (Nitkiewicz et al, 2014). Further work is needed to explore the role of orexin peptides in the control of uterine/placental functions.…”

Section: Discussionmentioning

confidence: 86%

“…Multiple lines of evidence have also demonstrated that orexins participate in the control of different endocrine axes, including the hypothalamicpituitary-ovarian axis (Silveyra et al, 2007a and2007b;Kaminski et al, 2010a and2010b;Nitkiewicz et al, 2010;Maleszka et al, 2013;Smolinska et al, 2014b). Local orexin production (Nitkiewicz et al, 2014) and the presence of specific receptors in porcine and rat ovaries (Silveyra et al, 2007a;Nitkiewicz et al, 2010) indicate that orexins could also exert direct endocrine and auto/paracrine effects inside glands. OXA was found in human and feline placenta, OX1R protein was identified in feline placenta (in syncytiotrophoblast and decidual cells derived from the endometrium), whereas the presence of OX2R protein was noted only in cells forming the endometrial glands (Nakabayashi et al, 2003;Dall'Aglio et al, 2012).…”

Section: Introductionmentioning

confidence: 99%

Expression of the orexin system in the porcine uterus, conceptus and trophoblast during early pregnancy

Smolińska

Kieżun

Szeszko

et al. 2015

Animal

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Orexin A and B are hypothalamic peptides derived from the prepro-orexin (PPO) precursor. Orexins stimulate food intake and arousal. Those peptides bind and activate two G protein-coupled receptors: orexin receptor 1 (OX1R) and orexin receptor 2 (OX2R). Numerous authors have suggested that orexins play an important role in the regulation of the reproductive functions. The objective of the present study was to analyse the presence of and changes in the gene and protein expression pattern of the orexin system in the porcine uterus, conceptus and trophoblast (chorioallantois) during early pregnancy. In the endometrium, the highest PPO and OX1R gene expression was detected on days 15 to 16 of gestation. The OX2R mRNA content in the endometrium was higher on days 10 to 11 and 15 to 16 than on days 12 to 13 and 27 to 28. In the trophoblasts, PPO gene expression was higher on days 30 to 32 than on days 27 to 28. The highest PPO protein content in the endometrium was noted on days 12 to 13. The highest OX1R protein content in the endometrium was detected on days 10 to 11, whereas OX2R protein on days 15 to 16. In the trophoblasts, PPO and OX1R protein levels were more pronounced on days 27 to 28 than on days 30 to 32, but OX2R expression was higher on days 30 to 32. The expression of PPO, OX1R and OX2R was different in the conceptuses and trophoblasts during early pregnancy. Local orexin production and the presence of the specific orexin receptors suggest that the orexin system may participate in the control of porcine reproductive functions by exerting endocrine and auto/paracrine effects on the uterus, conceptuses and trophoblasts during early pregnancy. This study provides the first evidence for the presence of orexins and their receptors in the uteri, conceptuses and trophoblasts in pigs during early pregnancy. The local orexin system is dependent on the stage of pregnancy.Keywords: orexin A and B, orexin receptors 1 and 2, uterus, pregnancy, pig ImplicationsThere is a growing body of evidence to implicate the existence of a common endocrine system that controls metabolism and the reproductive system. Orexin A and orexin B, the hormones that control energy homoeostasis, may be a part of the above system. This paper provides the pioneering information about the presence of orexins and orexin receptors in the porcine uterus, conceptus and trophoblast during early pregnancy, the crucial period for reproductive success. The expression levels of orexins and their receptors were found to be correlated with the stages of early pregnancy. A sound knowledge of mechanisms that control energy homoeostasis and reproduction is needed in animal breeding practice.

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“…Expressions of OXA and OX1R have also been demonstrated in rat ovaries, testes and in principal cells of epididymis and in urethra‐prostatic complex and vestibular glands of cattle (Johren et al ., ; Barreiro et al ., , ; Russo et al ., ; Pavone et al ., ; Tafuri et al ., , ; Assisi et al ., ). Further, expressions of OXA and OXB have also been reported in porcine ovary at different stages of oestrous cycle, and these orexins are shown to play a role in ovarian steroidogenesis (Nitkiewicz et al ., ). It is also reported that OXA may modulate steroidogenesis in the porcine uterus during early pregnancy and oestrous cycle (Kiezun et al ., ).…”

Section: Introductionmentioning

confidence: 97%

The neuropeptide orexin A – search for its possible role in regulation of steroidogenesis in adult mice testes

Joshi

Singh

2018

Andrology

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Orexin A, a hypothalamic neuropeptide, regulates food intake and sleep-wake cycle by binding to orexin receptor 1. Besides brain, orexin A and orexin receptor 1 are also present in peripheral organs. In our earlier studies, localization and expression of orexin A and orexin receptor 1 have been shown in adult mouse testis, and further their role in testicular development in neonatal mouse was also demonstrated. In this study, role of orexin A and orexin receptor 1 in the testis of adult mouse by blocking binding of orexin A to orexin receptor 1 using an orexin receptor 1 antagonist, SB-334867, was investigated under in vivo and ex vivo conditions. Mice were given a single bilateral intratesticular injection of the antagonist at doses of 4 and 12 μg/mouse and were sacrificed 24 h post-injection. The antagonist treatment caused degenerative changes in the seminiferous tubules in the testis and also caused alterations in steroidogenesis, with a concomitant decrease in the level of testosterone (T) and an increase in the level of 17β-estradiol (E ) in serum and in testis. Further, expressions of SF1, StAR, P450scc and 17β-HSD were downregulated, while the expressions of 3β-HSD and P450arom were upregulated in antagonist-treated mice compared with controls. Also, the oxidative stress in the testis was increased in treated mice. In ex vivo study, antagonist treatment to the testis caused a marked decrease in the level of T and an increase in the level of E in the media, accompanied by downregulated expression of SF1, StAR, P450scc and 17β-HSD and an upregulation in the expression of 3β-HSD and P450arom, indicating a direct role of orexin A in regulation of testicular steroidogenesis. The results of ex vivo study supported the findings of in vivo study. In conclusion, the results suggest potential involvement of orexin A and orexin receptor 1 in regulation of steroidogenesis and spermatogenesis in the testis of adult Parkes mice.

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Expression of orexins and their precursor in the porcine ovary and the influence of orexins on ovarian steroidogenesis in pigs

Cited by 28 publications

References 39 publications

Orexin A Expression in the Ovary of Dog and Cat

Orexin A Expression in the Ovary of Dog and Cat

Expression of the orexin system in the porcine uterus, conceptus and trophoblast during early pregnancy

The neuropeptide orexin A – search for its possible role in regulation of steroidogenesis in adult mice testes

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