Expression of Oocyte Vitellogenesis Receptor Was Regulated by C/EBPα in Developing Follicle of Wanxi White Goose

Abstract

Yolk precursor was synthesized under regulation of hormone secretion, while the mechanism of its incorporation into follicle is still unknown. The reproductive hormones, oocyte vitellogenesis receptor (OVR) expression at pre-, early-, peak- and ceased-laying period, and localization of Wanxi White goose were determined in this study. The results showed that the concentration of LH was lowest in serum at peak laying period compared to the other periods (p < 0.01). Moreover, the concentration of E2 was highest (p < 0.01) in serum at early laying period than that of other periods. Moreover, the gene expression level of OVR was highest at ceased laying period compared to other periods (p = 0.014) and was higher in developing follicles than other follicles (p < 0.01). The OVR was distributed in the granular cell layer and decreased with the maturation of follicles. Five transcription factors were predicted in the promoter of OVR, then were screened and verified by overexpression in granulosa cells. C/EBPα and MF3 significantly stimulated the expression of OVR. The combined overexpression of C/EBPα and OVR significantly stimulated the transportation of lipid from culture medium to cytoplasm. In conclusion, C/EBPα is the key transcription factor promoting OVR expression in goose follicle granulosa cells.

BMP4/SMAD8 signaling pathway regulated granular cell proliferation to promote follicle development in Wanxi white goose

“…Geese at their peak laying period were used for follicle collection and granular cell isolation, culture and identification was performed according to Du et al (2022) . The isolated granular cells were cultured in 6-well culture plate for plasmid transfection according to Du et al (2022) . The transfection efficiency was quantified using Image J software and presented as % transfected cells/NC cells.…”

FGF12 regulates cell cycle gene expression and promotes follicular granulosa cell proliferation through ERK phosphorylation in geese

“…The granulosa cell layer was removed by gently shaking and the rest was the theca layer. The isolation and culture of follicular granulosa cells of geese were performed according to Du et al (2022) . The isolated granular cells were cultured in 6-well plates for plasmid transfection.…”

“…The isolated granular cells were cultured in 6-well plates for plasmid transfection. Plasmid transfection was performed using the ExFect Transfection Reagent kit (T101-01, Vazyme, China) according to Du et al (2022) . The expression of gene mRNA and protein, as well as cell proliferation and apoptosis were analyzed 36 h after transfection.…”