Expression of NOX5 in human teratozoospermia compared to normozoospermia

Ghani, Esmaeel; Keshtgar, Sara; Habibagahi, Mojtaba; Ghannadi, Alireza; Kazeroni, M.

doi:10.1111/and.12023

Cited by 22 publications

(18 citation statements)

References 24 publications

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“…These are directly involved in the oxidative balance of spermatozoa with the latter being highly interrelated with peroxidative damage to the sperm cell [40,41]. Furthermore, Ghani et al [42] showed a significantly elevated expression of NOX5, a novel NADPH-oxidase and prime candidate for the ROS production in the acrosomal, equatorial, post-acrosomal regions of abnormal spermatozoa. Moreover, a significant positive correlation was observed between the NOX5 activity and the frequency of sperm with abnormal morphology.…”

Section: Discussionmentioning

confidence: 99%

Relationship amongst teratozoospermia, seminal oxidative stress and male infertility

Agarwal

Tvrdá

Sharma

2014

Reprod Biol Endocrinol

143

114

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BackgroundSpermatozoa morphology is an important and complex characteristic of the fertilization capacity of male germ cells. Morphological abnormalities have been observed to be accompanied by reactive oxygen species (ROS) overproduction and further damage to spermatozoa, ultimately leading to infertility. Therefore, this study aimed to examine the relationship between seminal ROS production and sperm morphology in infertile teratozoospermic patients as well as in healthy men of proven and unproven fertility.MethodsSemen samples were collected from 79 patients classified as teratozoospermic and 56 healthy donors (control). Standard semen analysis was performed and spermatozoa morphology was assessed according to the WHO 2010 guidelines. Seminal ROS was measured by chemiluminescence assay. Receiver operating characteristic (ROC) curves were generated, and sensitivity, specificity, cutoff value and area under curve (AUC) were determined.ResultsSperm morphology was significantly poor in the Teratozoospermic Group compared with the 3 Donor Groups (P < 0.05). Significantly higher levels of ROS (RLU/sec/106 sperm) were seen in the Teratozoospermic group (145.4 (41.5; 555.4) compared to the Donor Groups: All Donors (64.8 (21.1; 198.2), Proven Donors (58.8 (14.2; 79.2) and Proven Donors < 2 years (58.8 (14.2; 79.2) (P < 0.05). ROS correlated negatively with sperm concentration in the All Donor group (r = −0.354; P = 0.021) as well as in the Teratozospermic group (r −0.356; P = 0.002). Using ROC analysis, we established the cutoff values for concentration, morphology and ROS.ConclusionsThe incidence of teratozoospermia may be directly related to the overproduction of seminal ROS. Therefore, besides sperm concentration and motility, spermatozoa morphology should receive an equally important consideration in the overall assessment of male fertility.

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Section: Discussionmentioning

confidence: 99%

Relationship amongst teratozoospermia, seminal oxidative stress and male infertility

Agarwal

Tvrdá

Sharma

2014

Reprod Biol Endocrinol

143

114

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“…Leucocytes and morphologically abnormal spermatozoa are the main sources of ROS in semen (Garrido, Meseguer, Simon, Pellicer, & Remohi, ). NADPH oxidase 5 (NOX5) was detected as the source of ROS production in ejaculated human spermatozoa (Musset et al., ), and correlations were observed between abnormal sperm morphology and both the percentage of NOX5‐positive spermatozoa and the magnitude of NOX5 expression (Ghani, Keshtgar, Habibagahi, Ghannadi, & Kazeroni, ). Higher levels of seminal ROS and lower antioxidant potential were reported in men with idiopathic infertility than healthy fertile controls (Pasqualotto et al., ).…”

Section: Introductionmentioning

confidence: 99%

Oxidative stress status and sperm DNA fragmentation in fertile and infertile men

et al. 2017

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Evidence suggests that disturbing the balance between reactive oxygen species levels and antioxidant contents in seminal plasma leads to oxidative stress resulting in male infertility. This study was carried out to identifying clinical significance of seminal oxidative stress and sperm DNA fragmentation in treatment strategies of male infertility in southwest Iran. Sperm parameters, lipid peroxidation and activity of antioxidant enzymes were assessed in fertile (n = 105) and infertile (n = 112) men. Malondialdehyde (MDA) levels in seminal plasma were found to be higher significantly (p < .001) in patients. Superoxide dismutase (SOD) and glutathione peroxidase (GPx) activities in seminal plasma were significantly (p < .001) lower in infertile men. Significant negative correlations were observed between MDA levels and sperm motility and normal morphology. Spermatozoa with fragmented DNA were higher (p < .001) in infertile men and significantly correlated with MDA levels and SOD and GPx activities. MDA of 4.2 nmol/ml, SOD of 4.89 U/ml and GPx of 329.6 mU/ml were optimum cut-off limits to discriminate infertile patients from fertile men. The results show the leading role of oxidative stress in aetiology of male infertility in southwest Iran and indicate that evaluation of seminal antioxidant status and DNA integrity can be helpful in men attending infertility clinics during fertility assessment.

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“…NOX5 was discovered in 2001 in human testis, uterus, spleen, and lymph node (Banfi et al, 2001;Cheng et al, 2001). Some years later, it was found in equine (Sabeur and Ball, 2007), human (Musset et al, 2012;Ghani et al, 2013) and canine spermatozoa (Setyawan et al, 2016).…”

Section: Discussionmentioning

confidence: 99%

NADPH Oxidase 5 and Melatonin: Involvement in Ram Sperm Capacitation

Miguel-Jiménez

Pina-Beltrán

Gimeno-Martos

et al. 2021

Front. Cell Dev. Biol.

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Reactive oxygen species (ROS) play an essential role in mammalian sperm capacitation. NADPH oxidase 5 (NOX5) has been described as the main source of ROS production in some mammalian spermatozoa, such as human and equine. On the other hand, melatonin can decrease cellular ROS levels and regulates NOX activity in somatic cells. Therefore, the objectives of this work were (1) to identify NOX5 in ram spermatozoa and analyze its possible changes during in vitro capacitation and (2) to investigate the effect of melatonin on NOX5 expression and localization and on superoxide levels in capacitated ram spermatozoa. Protein bands associated with NOX5 were detected by Western blot analysis. Likewise, indirect immunofluorescence (IIF) revealed six different immunotypes for NOX5, which varied throughout in vitro capacitation. Superoxide (O2⋅–), evaluated by DHE/Yo-Pro-1, rose after in vitro capacitation and in the presence of the calcium ionophore A23187 but decreased in the presence of the NOX inhibitor GKT136901. GKT also reduced the percentage of capacitated and acrosome-reacted spermatozoa that had increased during incubation in capacitating conditions. The presence of melatonin at micromolar concentrations avoided the increment in O2⋅– and the changes in NOX5 immunotypes provoked by capacitation. In conclusion, NOX5 is present in ram spermatozoa and the changes in its distribution, associated with sperm capacitation, can be prevented by melatonin. To this extent, it could imply that melatonin exerts its antioxidant role, at least in part, by modulating NOX5 activity during ram sperm capacitation.

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Expression of NOX5 in human teratozoospermia compared to normozoospermia

Cited by 22 publications

References 24 publications

Relationship amongst teratozoospermia, seminal oxidative stress and male infertility

Relationship amongst teratozoospermia, seminal oxidative stress and male infertility

Oxidative stress status and sperm DNA fragmentation in fertile and infertile men

NADPH Oxidase 5 and Melatonin: Involvement in Ram Sperm Capacitation

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