Expression of novel lhmlt fusion protein using plant viral vector and study of its anticancer effect

Naseri, Zahid; Uliaei, Ebrahim Dorani; Ofoghi, Hamideh; Davarpanah, Seyed Javad

doi:10.1007/s11240-020-01900-8

Cited by 2 publications

(1 citation statement)

References 65 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…In another example, PVX and a P19 suppressor were used to express a fusion protein (lhmlt) of melittin peptide and gonadotropin-releasing hormone receptor (GnRHR) in N. benthamiana. The purified protein was functional and inhibited the cancerous cells in the MTT assay ( Naseri et al., 2020 ). In a recent study, CRISPR/Cas9 was also employed to knockout N. benthamiana dicer-like proteins 2 and 4 (NbDCL2 and NbDCL4).…”

Section: Heterologous Expression Of Recombinant Proteins In Plantsmentioning

confidence: 99%

Plant-based expression platforms to produce high-value metabolites and proteins

Kulshreshtha¹,

Sharma

Padilla³

et al. 2022

Front. Plant Sci.

View full text Add to dashboard Cite

Plant-based heterologous expression systems can be leveraged to produce high-value therapeutics, industrially important proteins, metabolites, and bioproducts. The production can be scaled up, free from pathogen contamination, and offer post-translational modifications to synthesize complex proteins. With advancements in molecular techniques, transgenics, CRISPR/Cas9 system, plant cell, tissue, and organ culture, significant progress has been made to increase the expression of recombinant proteins and important metabolites in plants. Methods are also available to stabilize RNA transcripts, optimize protein translation, engineer proteins for their stability, and target proteins to subcellular locations best suited for their accumulation. This mini-review focuses on recent advancements to enhance the production of high-value metabolites and proteins necessary for therapeutic applications using plants as bio-factories.

show abstract

Section: Heterologous Expression Of Recombinant Proteins In Plantsmentioning

confidence: 99%